merck millipore,默克密理博,04-1127,Anti-ErbB2/Her2 Antibody, clone EP1045Y, rabbit monoclonal

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merck millipore,默克密理博,04-1127,Anti-ErbB2/Her2 Antibody, clone EP1045Y, rabbit monoclonal
产品名称：Anti-ErbB2/Her2 Antibody, clone EP1045Y, rabbit monoclonal
产品型号：04-1127
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. This Anti-ErbB2/Her2 Antibody, clone EP1045Y, Rabbit More>>

merck millipore,默克密理博,04-1127,Anti-ErbB2/Her2 Antibody, clone EP1045Y, rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-1127,Anti-ErbB2/Her2 Antibody, clone EP1045Y, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
H	FC, WB	Rb	Unpurified	Monoclonal Antibody

描述
产品目录编号	04-1127
描述	Anti-ErbB2/Her2 Antibody, clone EP1045Y, rabbit monoclonal
Alternate Names	v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) CD340 antigen MLN 19 NEU proto-oncogene Tyrosine kinase-type cell surface receptor HER2 c-erb B2/neu protein neuroblastoma/glioblastoma derived oncogene homolog v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog)
背景信息	ErbB2/Her2 is a member of the ErbB receptor family of tyrosine kinases. ErbB2 can be activated without a ligand if it is overexpressed and can be detected in approximately 40% of human breast cancers. ErbB2 is a major target that is being explored In the treatment of breast cancer and other carcinomas.

产品信息
格式	Unpurified
控制	SKBR-3 cell lysate
演示	Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. This Anti-ErbB2/Her2 Antibody, clone EP1045Y, Rabbit is validated for use in WB, FC, IH(P), IP for the detection of ErbB2/Her2.
主要应用	Flow Cytometry Western Blotting
应用说明	Immunohistochemistry Analysis: A 1:100-250 dilution from a previous lot detected ErbB2/Her2 in human breast carcinoma tissue. Flow Cytometry: A 1:30 dilution of a previous lot was used in flow cytometry. Immunoprecipitation Analysis: A 1:50 dilution from a previous lot was used in IP.

生物信息
免疫原品种	Synthetic peptide corresponding to residues near the C-terminus of human Her2.
表位	C-terminus
克隆	EP1045Y
宿主	Rabbit
特异性	This antibody detects Her2 phosphorylated at Tyr1248 as well as unphosphorlyated Her2.
同种型	IgG
品种反应性	Human
Species Reactivity Note	Human
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_001005862.1
基因符号	p185erbB2 C-erbB-2 CD340 EC 2.7.10.1 HER-2 HER-2/neu HER2 NEU NGL TKR1 erbB-2 herstatin
纯化方法	Unpurified
UniProt编号	P04626
UniProt汇总	FUNCTION: Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Not activated by EGF, TGF-alpha and amphiregulin. CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. SUBUNIT STRUCTURE: Heterodimer with each of the other ERBB receptors Potential. Interacts with PRKCABP and PLXNB1. Part of a complex with EGFR and either PIK3C2A or PIK3C2B. May interact with PIK3C2B when phosphorylated on Tyr-1196. Interacts with MEMO when phosphorylated on Tyr-1248. Interacts with MUC1. Stimulation by heregulin (HRG) in breast cancer cell lines induces binding of MUC1 with gamma-catenin. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. PTM: Ligand-binding increases phosphorylation on tyrosine residues By similarity. Polymorphism There are fours alleles due to the variations in positions 654 and 655. Allele B1 (Ile-654/Ile-655) has a frequency of 0.782; allele B2 (Ile-654/Val-655) has a frequency of 0.206; allele B3 (Val-654/Val-655) has a frequency of 0.012. Involvement in disease Defects in ERBB2 are associated with gastric cancer [MIM:137215]; also known as hereditary familial diffuse gastric cancer (HDGC). Defects in ERBB2 are associated with familial glioma of brain [MIM:137800]; also called glioblastoma multiforme. Gliomas are central nervous system neoplasms derived from glial cells and comprise astrocytomas, glioblastoma multiforme, oligodendrogliomas, and ependymomas. Defects in ERBB2 are associated with ovarian cancer [MIM:167000]. Ovarian cancer is the leading cause of death from gynecologic malignancy. It is characterized by advanced presentation with loco-regional dissemination in the peritoneal cavity and the rare incidence of visceral metastases. These typical features relate to the biology of the disease, which is a principal determinant of outcome. Defects in ERBB2 are associated with lung cancer [MIM:211980]; also called adenocarcinoma of lung. SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. Contains 1 protein kinase domain.

产品使用声明
质量保证	Evaluated by Western Blot on SKBR-3 cell lysates. Western Blot Analysis: A 1:50,000-100,000 dilution of this antibody was used to detect ErbB2/Her2 in SKBR-3 cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.