merck millipore,默克密理愽
  • merck millipore,默克密理博,FCSC100107,FlowCellect™ Human iPS Cell Characterization Kit

  • merck millipore,默克密理博,FCSC100107,FlowCellect™ Human iPS Cell Characterization Kit

    产品名称：FlowCellect™ Human iPS Cell Characterization Kit
    产品型号：FCSC100107
    This FlowCellect Human iPS Cell Characterization Kit for flow cytometry provides a quantitative solution for clearly identifying human iPSC cultures using flow cytometry.

    merck millipore,默克密理博,FCSC100107,FlowCellect™ Human iPS Cell Characterization Kit

  • 产品介绍
  • merck millipore,默克密理博,FCSC100107,FlowCellect™ Human iPS Cell Characterization Kit

    描述
    产品目录编号 FCSC100107
    商名
    • FlowCellect
    描述 FlowCellect™ Human iPS Cell Characterization Kit
    概述 Millipore’s FlowCellect™ Human iPS Cell Characterization Kit provides a quantitative solution for clearly identifying human iPSC cultures using flow cytometry. The kit contains four directly conjugated antibodies to phenotypically characterize human iPSCs, with three positive expressing markers (Tra-1-60, SSEA4, and human Oct4), as well as one negative marker, SSEA1. These antibodies have been carefully evaluated and optimized on human induced pluripotent stem cells and human ES derived oligodendrocyte progenitor cells (negative control), ensuring that the kit can clearly monitor and characterize human iPSC cultures.

    All kit antibodies are optimized for guava® bench top flow cytometers and provide a complete solution for human induced pluripotent stem cell analysis. However, this kit can be used on any flow cytometer following the same protocol, offering researchers a reliable, quantitative, and fully validated solution to study their iPSC cultures. All four directly conjugated antibodies provided in the kit have been carefully evaluated to ensure optimal performance for flow cytometry, alleviating the need for any additional validation of the kit reagents. Also, plate-based stability testing and quality control was performed to confirm the stability of the antibodies over time. After 120 minutes (2 hours) post sample preparation, kit antibodies did not lose activity (< 5-10% of signal strength). So many tests can be performed in a single experiment without the risk of losing antibody activity or performance over time.
    Alternate Names
    • Flow Cellect
    背景信息 Human induced pluripotent stem cells (iPS cells) are somatic cells that have been genetically reprogrammed to an embryonic stem cell-like state by being forced to express genes and factors important for maintaining the defining properties of embryonic stem cells. Because of their ability to mimic human embryonic stem cells (hESCs) with pluripotent capabilities and the ability to differentiate into any cell types, it is generally believed that iPS cells have the potential for alleviating incurable diseases and aiding organ transplantation [1]. Human iPS cells are useful tools for modeling diseases and for drug development, and by having the ability to develop “custom made” adult cells derived from iPS cells this may ultimately allow the treatment of patients with debilitating degenerative disorders [2]. In fact, some iPSC-based therapies are already being examined in animal models and showing signs of therapeutic utility [2,3].

    In 2012, the Nobel Prize for Medicine was awarded to Sir John B. Gurden and Shinya Yamanaka for their work on iPS cells. In Takahashi and Yamanaka (2006), it examined if genetic reprogramming using specific transcription factors could induce pluripotency in somatic cells. And based on their findings, they were able to demonstrate that pluripotent stem cells can be directly generated from fibroblast cultures by the addition of only a few defined factors, a technique which has revolutionized the stem cell market [4]. The reprogramming of human cells allow scientists new opportunities to study diseases and develop methods for diagnosis without the need of embryonic stem cells. The use of embryonic stem cells (ESCs) have been controversial due to its origin (e.g. blastocytes) since it requires the destruction of the fertilized human embryo. This in itself has raised many ethical issues, hence, providing the need to find an alternative source of pluripotent stem cells with the same range of functions as ES cells [1,4]. Like human embryonic stem cells, iPS cells have the capacity to generate cells from each of the three germ layers (endoderm, mesoderm, and ectoderm), but also can be propagated in culture indefinitely [4,5,9].
    产品信息
    部件
    • Permeabilizaton Buffer: (Part No. CS202125) One bottle containing 13 mL
    • 20X Anti-Tra-1-60/FITC, clone TRA-1-60: (Part No. CS211422). One vial containing 500 mL
    • 20X Anti-SSEA4/PE, clone MC-813-70: (Part No. CS211423). One vial containing 500 mL
    • 20X Anti-SSEA1/PECy5, clone MC-480: (Part No. CS210082). One vial containing 500 mL
    • 20X Anti-human Oct4/Alexa Fluor®647, clone 10H11.2: (Part No. CS211425). One vial containing 500 mL
    • 5X Assay Buffer: (Part No. CS202124) One bottle containing 55 mL
    • Fixation Buffer: (Part No. CS202122) One bottle containing 13 mL
    检测方法 Fluorescence
    应用
    应用 This FlowCellect Human iPS Cell Characterization Kit for flow cytometry provides a quantitative solution for clearly identifying human iPSC cultures using flow cytometry.
    主要应用
    • Flow Cytometry
    生物信息
    宿主 Human
    品种反应性 Human
    基因符号
    • OCT4
    • Tra-1-60
    • SSEA4
    • FUT4
    产品使用声明
    质量保证 Lot to lot in-process testing on all kit components
    使用声明
    • Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 4-8°C; 4 Months
    包装信息
    数量 100 Tests / Kit

    merck millipore,默克密理博,FCSC100107,FlowCellect™ Human iPS Cell Characterization Kit

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