merck millipore,默克密理博,09-018,Anti-phospho Histone Macro H2A (Ser137) Antibody

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merck millipore,默克密理博,09-018,Anti-phospho Histone Macro H2A (Ser137) Antibody
产品名称：Anti-phospho Histone Macro H2A (Ser137) Antibody
产品型号：09-018
Use Anti-phospho Histone Macro H2A (Ser137) Antibody (rabbit polyclonal antibody) validated in WB, ICC, Mplex to detect phospho Histone Macro H2A (Ser137) also known as Histone macroH2A1, Medulloblast More>>

merck millipore,默克密理博,09-018,Anti-phospho Histone Macro H2A (Ser137) Antibody

产品介绍

merck millipore,默克密理博,09-018,Anti-phospho Histone Macro H2A (Ser137) Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	ICC, WB	Rb	Affinity Purified	Polyclonal Antibody

描述
产品目录编号	09-018
描述	Anti-phospho Histone Macro H2A (Ser137) Antibody
Alternate Names	histone macroH2A1.2 H2A histone family, member Y Histone macroH2A1 Medulloblastoma antigen MU-MB-50.205 histone macroH2A1.1 histone macroH2A1.1
背景信息	Histone MacroH2A (mH2A), also known as H2AFY, is a vertebrate-specific histone variant that is associated with condensed, inactive heterochromatin. mH2A is enriched in chromatin of the female inactive X chromosome and in highly methylated CpG loci. mH2A has been shown to repress RNA polymerase II-driven transcription at the level of transcriptional initiation. There are two phosphorylation sites within the basic hinge region of mH2A: Thr128 and Ser137. Phospho-Ser137 is enriched in mitosis, implicating mH2A in the regulation of post-translational modifications throughout the cell cycle.

产品信息
格式	Affinity Purified
控制	Nocodazole treated and untreated HeLa acid extract
演示	Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.150 mM NaCl and 0.05% sodium azide.

应用
应用	Use Anti-phospho Histone Macro H2A (Ser137) Antibody (rabbit polyclonal antibody) validated in WB, ICC, Mplex to detect phospho Histone Macro H2A (Ser137) also known as Histone macroH2A1, Medulloblastoma antigen MU-MB-50.205.
主要应用	Immunocytochemistry Western Blotting
应用说明	Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Histone Macro H2A in NIH/3T3, HeLa, and A431 cells.

生物信息
免疫原品种	Linear peptide corresponding to human Histone Macro H2A at Ser137.
表位	Ser137
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
特异性	This antibody recognizes Histone Macro H2A phosphorylated at Ser137.
品种反应性	RatHumanMouse
Species Reactivity Note	Demonstrated to react with human and mouse. Predicted to react with all mammals based on 100% sequence homology.
抗体类型	Polyclonal Antibody
Entrez基因编号	NP_004884.1
Entrez基因汇总	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a member of the histone H2A family. It replaces conventional H2A histones in a subset of nucleosomes where it represses transcription and participates in stable X chromosome inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms. Transcript Variant: This variant (2) has a unique 5' UTR and uses an alternate splice site in its coding region, compared to variant 3. This results in a protein (isoform 2) that is a single amino acid shorter than isoform 3. Variants 2 and 4 encode the same isoform (2).
基因符号	macroH2A1.2 H2A.y H2A/y H2AF12M H2AFJ MACROH2A1 MACROH2A1.1 mH2A1
修改	Phosphorylation
纯化方法	Affinity Purfied
UniProt编号	O75367
UniProt汇总	FUNCTION: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation. Inhibits the binding of transcription factors and interferes with the activity of remodeling SWI/SNF complexes. Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces an hypoacetylated state of chromatin. In addition, isoform 1, but not isoform 2, binds ADP-ribose and O-acetyl-ADP-ribose, and may be involved in ADP-ribose-mediated chromatin modulation. SUBUNIT STRUCTURE: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. Interacts with SPOP. Part of a complex consisting of H2AFY, CUL3 and SPOP. Interacts with HDAC1 and HDAC2 By similarity. SUBCELLULAR LOCATION: Nucleus. Note= Enriched in inactive X chromosome chromatin and in senescence-associated heterochromatin. TISSUE SPECIFICITY: Ubiquitous. PTM: Monoubiquitinated at either Lys-116 or Lys-117. May also be polyubiquitinated. Ubiquitination is mediated by the CUL3/SPOP E3 complex and does not promote proteasomal degradation. Instead, it is required for enrichment in inactive X chromosome chromatin. MISCELLANEOUS: The number of individuals with antibodies against this antigen is 2-fold higher in pediatric patients with cancer compared to healthy controls. SIMILARITY: Contains 1 histone H2A domain. Contains 1 Macro domain.

产品使用声明
质量保证	Evaluated by Western Blot in Nocodazole treated and untreated HeLa acid extract. Western Blot Analysis: 0.1 µg/ml of this antibody detected Histone Macro H2A on 10 µg of Nocodazole treated and untreated HeLa acid extract.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Maintain refrigerated at 2-8°C for up to 6 months.

包装信息
数量	100 µL

merck millipore,默克密理博,09-018,Anti-phospho Histone Macro H2A (Ser137) Antibody

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