merck millipore,默克密理博,AB902,Anti-Adrenocorticotropic Hormone Antibody

搜索"Merck Millipore 密理愽"产品

Merck Millipore 默克密理愽

当前位置：首页 > >Merck Millipore > 生命科学研究
生命科学研究

merck millipore,默克密理博,AB902,Anti-Adrenocorticotropic Hormone Antibody
产品名称：Anti-Adrenocorticotropic Hormone Antibody
产品型号：AB902
Anti-Adrenocorticotropic Hormone Antibody detects level of Adrenocorticotropic Hormone & has been published & validated for use in IH(P).

merck millipore,默克密理博,AB902,Anti-Adrenocorticotropic Hormone Antibody

产品介绍

merck millipore,默克密理博,AB902,Anti-Adrenocorticotropic Hormone Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
Fe, B, Ca, H, R, Sh	IH(P)	Rb	Serum	Polyclonal Antibody

描述
产品目录编号	AB902
品牌系列	Chemicon®
商名	Chemicon
描述	Anti-Adrenocorticotropic Hormone Antibody
Alternate Names	ACTH
背景信息	ANTIGEN: Synthetic ACTH (1-24) bound to keyhole limpet hemocyanin (KLH) with carbodiimide.

产品信息
格式	Serum
演示	Liquid. Contains 0.1% sodium azide.

应用
应用	Anti-Adrenocorticotropic Hormone Antibody detects level of Adrenocorticotropic Hormone & has been published & validated for use in IH(P).
主要应用	Immunohistochemistry (Paraffin)
应用说明	TISSUE EVALUATION: Formalin-fixed and paraffin-embedded sections (6 microns thick) of anterior pituitary glands were prepared from a number of species including man, dog, rat, cat, ox and sheep. Cryostat sections of the above tissued can also be used in the staining procedures. IMMUNOPEROXIDASE STAINING: 1. The sections to be stained are hydrated to phosphate buffered saline (PBS, pH 7.4). 2. The antiserum diluted 1:1,000 - 1:2,000 (in PBS) is allowed to react with the tissue at 37° C for 30 minutes. 3.Using PBS, the tissue is washed for 15 min at room temperature and treated sequentially with sheep anti-rabbit gamma globulin (diluted 1:20 in PBS) and peroxidase anti-peroxidase complex (diluted 1:100 in PBS) for 37°C. After each of these two steps excess reagent is washed from the section as described above. 4. The final step in the staining involves treating the section with a mixture of 3,3'- diaminbenzidine HCl (0.02%) in hydrogen peroxide (0.004%). For preservation, the sections are dehydrated, cleared and mounted. IMMUNOFLUORESCENCE STAINING: 1. Same as Step 1 above. 2. The tissue is reacted with the antiserum, diluted 1:20-1:40 in (PBS) for 30 minutes at 37°C. 3. After washing off the excess reagents with PBS, the section is treated with fluoresceinlabeled sheep anti-rabbit gamma globulin (diluted 1:10 in PBS) for 30 min at 37° C. The tissue is washed with PBS and after mounting under glycerine-saline is viewed with a fluorescence microscope. COMMENTS: The antiserum dilutions described have been established as giving a positive staining on the corticotrophs using the above protocol. It is recommended, however, that the customer establishes his own optimal dilutions to give positive staining. In general, staining can occur on further dilution of the primary antiserum if this is accompanied by an increase in its incubation time e.g. 24 hr.

生物信息
宿主	Rabbit
特异性	The antiserum provided has been evaluated for its ability to give a specific and positive immunostaining of ACTH-containing cells using both the immunoperoxidase and immunofluoresence procedures.
品种反应性	FelineBovineCanineHumanRatSheep
抗体类型	Polyclonal Antibody
Entrez基因编号	NM_001035256.1 NM_000939.2
Entrez基因汇总	This gene encodes a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the polypeptide precursor and, depending on tissue type and the available convertases, processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used; adrenocorticotrophin, essential for normal steroidogenesis and the maintenance of normal adrenal weight, and lipotropin beta are the major end products. In other tissues, including the hypothalamus, placenta, and epithelium, all cleavage sites may be used, giving rise to peptides with roles in pain and energy homeostasis, melanocyte stimulation, and immune modulation. These include several distinct melanotropins, lipotropins, and endorphins that are contained within the adrenocorticotrophin and beta-lipotropin peptides. Mutations in this gene have been associated with early onset obesity, adrenal insufficiency, and red hair pigmentation. Alternatively spliced transcript variants encoding the same protein have been described.
基因符号	gamma-MSH POMC pro-ACTH-endorphin adrenocorticotropin beta-LPH POC gamma-LPH LPH ACTH corticotropin beta-endorphin beta-MSH alpha-MSH pro-opiomelanocortin proopiomelanocortin Pro-opiomelanocortin corticotropin-lipotrophin NPP MSH met-enkephalin CLIP
UniProt编号	P01189
UniProt汇总	FUNCTION: SwissProt: P01189 # Beta-endorphin and Met-enkephalin are endogenous opiates. SIZE: 267 amino acids; 29424 Da TISSUE SPECIFICITY: ACTH and MSH are produced by the pituitary gland. PTM: Specific enzymatic cleavages at paired basic residues yield the different active peptides. & O-glycosylated; reducing sugar is probably N- acetylgalactosamine. DISEASE: SwissProt: P01189 # Defects in POMC may be associated with susceptibility to obesity [MIM:601665]. & Defects in POMC are the cause of pro-opiomelanocortinin deficiency [MIM:609734]. Affected individuals present early-onset obesity, adrenal insufficiency and red hair. SIMILARITY: SwissProt: P01189 ## Belongs to the POMC family.

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Store at -20°C in undiluted aliquots for up to 12 months. Do not thaw and refreeze material.

包装信息
数量	500 µL

merck millipore,默克密理博,AB902,Anti-Adrenocorticotropic Hormone Antibody

上一件merck millipore产品：merck millipore,默克密理博,ABD51,Anti-BMPR-1A Antibody
下一件merck millipore产品：merck millipore,默克密理博,07-631,Anti-cdk2 Antibody

whatman,沃特曼,nalgene,耐洁,merck millipore,默克密理愽,sartorius,赛多利斯,pall,颇尔
thermo fisher,赛默飞世尔,GE,MN,macherey-nagel,advantec,wheaton,brand,普兰德,welch,airtech