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品种反应性 主要应用 宿主 格式 抗体类型 H WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 07-883 品牌系列 Upstate
商名 - Upstate
描述 Anti-phospho-PKCθ (Ser676) Antibody Alternate Names - KPCT
- PRKCQ
- PKCq
- protein kinase C, theta
- PRKCT
- PRKCT
产品信息 格式 Affinity Purified 演示 100 μL of affinity purified rabbit polyclonal IgG in 50% storage buffer (PBS (without Mg2+ and Ca2+), pH 7.3 containing 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide). 应用 应用 Detect phospho-PKCθ (Ser676) using this Anti-phospho-PKCθ (Ser676) Antibody validated for use in WB. 主要应用 - Western Blotting
应用说明 Immunoblot Analysis: A 0.50 μg/ml dilutions of this lot detected phospho-PKCθ (Ser676) in RIPA lysate of PMA treated Jurkat cells. 生物信息 免疫原品种 Peptide corresponding to amino acid region encompassing the human phospho-PKCθ (Ser676). 宿主 Rabbit 特异性 Recognizes phosphorylated PKCθ (Ser676). Does not cross-react with other PKC isoforms. 同种型 IgG 品种反应性 Human Species Reactivity Note Predicted to cross-react with mouse based on sequence homology, but have not been tested. 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_006257.2
Entrez基因汇总 Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors. 基因符号 - nPKC-theta
- PRKCQ
- OTTHUMP00000043364
- OTTHUMP00000043365
- PRKCT
- MGC126514
- MGC141919
修改 - Phosphorylation
纯化方法 Affinity Purfied UniProt编号 - Q04759
UniProt汇总 FUNCTION: SwissProt: Q04759 # PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
COFACTOR: Magnesium.
SIZE: 706 amino acids; 81865 Da
SUBUNIT: Interacts with TXNL2/PICOT.
TISSUE SPECIFICITY: Skeletal muscle, megakaryoblastic cells and platelets.
DOMAIN: SwissProt: Q04759 The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
PTM: Autophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation.
SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 C2 domain. & Contains 2 phorbol-ester/DAG-type zinc fingers. & Contains 1 protein kinase domain.产品使用声明 质量保证 Routinely evaluated by immunoblot. 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 2 years at -80°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -80°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. 包装信息 数量 100 µL
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