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重要规格表
检测方法 Colorimetric 描述 概述 A spectrophotometric assay kit used for the measurement of reduced to oxidized glutathione ratio (GSH/GSSG) in biological samples. 产品目录编号 371757 品牌系列 Calbiochem®
特点及优点 • Highly Sensitive and Specific: Lower limit of detection in sample mixture is 9 nM GSSG. Over 13 different compounds were tested to assess any interference and exhibited no interference.
• Easy to Use: A simplified colorimetric assay. the GSH/GSSG assay uses the thiol-scavenging reagent that rapidly scavenges GSH, but does not interfere with glutathione reductase assay. Each kit can be used for up to 100 tests for determining total glutathione/GSSG ratios or 200 tests for either of the two.
Assay time: About 1.5 h
• Versatile: Can be used with whole blood, cell lysates, and tissue extracts.应用数据 
A four point calibration curve A412/min VS. µ GSH used to determine the concentration of GSSG.
Stability of GSSG, from a rested subject, in the intact red blood cell at 4°C. (GSH-159).
Stability of GSSG in the red blood cell lysate at 4°C. (GSH-161)参考 参考 Anderson, M. 1996. Glutathione in Free Radicals, A Practical Approach, ed. N.A. Punchard and F.J. Kelly Oxford University Press, New York, p 213.
Richie, J.P. Jr., et al. 1996. Clinical Chemistry 42, 64.
Griffith, O.W. 1980. Analytical Biochemistry 106, 207.
Tietze, F. 1969. Analytical Chemistry 27, 502.
Guntherberg, H., and Rost, J. 1966. Analytical Biochemistry 15, 205.产品信息 检测方法 Colorimetric 形式 200 Tests 格式 Cuvette 试剂盒包含 Chromogen DTNB, Glutathione Reductase Enzyme, NADPH, Scavenger, Assay Buffer, GSSG Buffer, Standard, and a user protocol. Each kit can be used to perform 100 tests representing the ratio of total Glutathione to the oxidized form or 200 tests of either. 生物信息 化验时间 1.5 h 样品类型 cell lysates, tissue extracts, blood 理化信息 灵敏度 9 nM GSSG 储存和货运信息 货运代码 Blue Ice Only
毒性 Standard Handling
储存 +2°C to +8°C
存储条件 When not in use, place the bottles at 4°C. Do not allow the capped reagent bottles to sit at room temperature for long periods of time. 避光 Protect from light
无需冷冻 No
补充信息 试剂盒包含 Chromogen DTNB, Glutathione Reductase Enzyme, NADPH, Scavenger, Assay Buffer, GSSG Buffer, Standard, and a user protocol. Each kit can be used to perform 100 tests representing the ratio of total Glutathione to the oxidized form or 200 tests of either.
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