merck millipore,默克密理博,07-702,Anti-FOXO3a/FKHRL1 Antibody

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merck millipore,默克密理博,07-702,Anti-FOXO3a/FKHRL1 Antibody
产品名称：Anti-FOXO3a/FKHRL1 Antibody
产品型号：07-702
Use Anti-FOXO3a/FKHRL1 Antibody (Rabbit Polyclonal Antibody) validated in WB to detect FOXO3a/FKHRL1 also known as AF6q21 protein, Forkhead in rhabdomyosarcoma-like 1, forkhead box O3A.

merck millipore,默克密理博,07-702,Anti-FOXO3a/FKHRL1 Antibody

产品介绍

merck millipore,默克密理博,07-702,Anti-FOXO3a/FKHRL1 Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
M, H	WB	Rb	Purified	Polyclonal Antibody

描述
产品目录编号	07-702
Replaces	04-1007
品牌系列	Upstate
商名	Upstate
描述	Anti-FOXO3a/FKHRL1 Antibody
Alternate Names	forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1 AF6q21 protein Forkhead in rhabdomyosarcoma-like 1 forkhead box O3 forkhead box O3A forkhead homolog (rhabdomyosarcoma) like 1 forkhead Drosophila homolog of
背景信息	The forkhead family of transcription factors is a large group of proteins that share a common conserved 100 amino acid DNA binding domain, which is termed a winged-helix or forkhead domain after the founding member of this group, the forkhead gene in Drosophila. In addition to their roles during normal development, recent studies have demonstrated that members of the forkhead family may participate in several kinds of neoplasia. In particular, 3 genes of the human forkhead family, FKHR, FKHRL1, and AFX, which are orthologues of DAF-16 in Caenorhabditis elegans, were identified at the chromosomal breakpoints in human neoplasms. These three genes have been assigned to the FOXO (named for “Forkhead Box, group O”) subfamily of forkhead transcription factors. The forkhead factors, including FKHRL1, regulate their activation through phosphorylation by proto-oncogene Akt (protein kinase B) and/or SGK (serum- and glucocorticoid- induced kinases) in mammalian cells and the phosphorylated form localized at the cytoplasm is recognized to be an inactivated form, whereas nuclear-imported dephosphorylated FKHRL1 is functional as an active transcriptional factor.

产品信息
格式	Purified
控制	3T3/A31 Cell Lysate.
演示	Antigen affinity purified rabbit polyclonal, followed by anti-GST depletion, in 0.1M Tris-Glycine (pH7.4), 150mM NaCl with 0.05% sodium azide.

应用
应用	Use Anti-FOXO3a/FKHRL1 Antibody (Rabbit Polyclonal Antibody) validated in WB to detect FOXO3a/FKHRL1 also known as AF6q21 protein, Forkhead in rhabdomyosarcoma-like 1, forkhead box O3A.
主要应用	Western Blotting

生物信息
免疫原品种	GST fusion protein corresponding to residues 330-673 of human FKHRL1/FOXO3A.
表位	a.a. 330-673
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
特异性	Recognizes FKHRL1/FOXO3A, Mr 90-95 kDa.
同种型	IgG
品种反应性	MouseHuman
Species Reactivity Note	Proven to react with human and mouse. Not tested on other species.
抗体类型	Polyclonal Antibody
Entrez基因编号	NM_001455
Entrez基因汇总	This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed.
基因符号	DKFZp781A0677 FOXO3 FKHRL1 FOXO2 MGC12739 FKHRL1P2 AF6q21 MGC31925 FOXO3A
纯化方法	Affinity Purfied
UniProt编号	O43524
UniProt汇总	FUNCTION: SwissProt: O43524 # Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. SIZE: 673 amino acids; 71277 Da SUBUNIT: Interacts with YWHAB/14-3-3-beta and YWHAZ/14-3-3-zeta, which are required for cytosolic sequestration. Upon oxidative stress, interacts with STK4, which disrupts interaction with YWHAB/14-3-3-beta and leads to nuclear translocation. SUBCELLULAR LOCATION: Cytoplasm, cytosol. Note=Translocates to the nucleus upon oxidative stress and in the absence of survival factors. TISSUE SPECIFICITY: Ubiquitous. PTM: In the presence of survival factors such as IGF-1, phosphorylated on Thr-32 and Ser-253 by AKT1/PKB. This phosphorylated form then interacts with 14-3-3 proteins and is retained in the cytoplasm. Survival factor withdrawal induces dephosphorylation and promotes translocation to the nucleus where the dephosphorylated protein induces transcription of target genes and triggers apoptosis. Although AKT1/PKB doesn't appear to phosphorylate Ser-315 directly, it may activate other kinases that trigger phosphorylation at this residue. Phosphorylated by STK4 on Ser-209 upon oxidative stress, which leads to dissociation from YWHAB/14-3-3-beta and nuclear translocation.DISEASE:SwissProt: O43524 # A chromosomal aberration involving FOXO3 is found in secondary acute leukemias. Translocation t(6;11)(q21;q23) with MLL/HRX. SIMILARITY: SwissProt: O43524 ## Contains 1 fork-head DNA-binding domain.

产品使用声明
质量保证	Routinely evaluated by Western Blot on 3T3/A31 cell lysates. Western Blot Analysis: A 0.5 g/mL dilution of this antibody was used to detect FOXO3a/FKHRL1 in 3T3/A31 cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at 2-8ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.

包装信息
数量	100 µg

merck millipore,默克密理博,07-702,Anti-FOXO3a/FKHRL1 Antibody

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