- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB6010 Replaces AB19052 描述 Anti-MMP-12 Antibody, NT (active) Alternate Names - matrix metallopeptidase 12
- Macrophage elastase
- Matrix metalloproteinase-12
- macrophage metalloelastase
背景信息 Matrix metallopeptidase 12 (MMP-12) is able to degrade elastin, entactin, laminin-1, fibronectin, type IV collagen as well as insulin B-chain and casein. MMP-12 is structurally similar to the classical MMPs (MMP-1, MMP-3); it contains a propeptide with autoinhibitory cysteine switch site, a well conserved zinc site, hinge region and hemopexin domain. MMP-12 lacks a transmembrane domain and furin cleavage site. The zymogen for MMP-12 is about 54 kDa, and is quickly activated to the 45 kDa form, and this breaks down to cascade of active forms, ending with the common 22 kDa form.Stimulated macrophages produce MMP-12; it has also been found in osteosarcoma cells, synovial fibroblasts and lung fibroblasts. MMP-12 levels in quiescent cells and tissues are minimal (with the exception of macrophages), and mitogen stimulation or protein concentration is often needed to visualize the bands. In addition, cell types differ greatly in the quantity of MMP-12 produced. 产品信息 格式 Affinity Purified 控制 - Concentrated conditioned cell culture media collected from a proprietary human fibroblast cell line.
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. 应用 应用 Anti-MMP-12 Antibody, N-term (active) is an antibody against MMP-12 for use in WB. 主要应用 - Western Blotting
生物信息 免疫原品种 KLH-conjugated synthetic peptide corresponding to human MMP-12 near the N-terminus. 表位 N-terminus 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes MMP-12 near the N-terminus of the active form. 品种反应性 Human Species Reactivity Note Human. Sequence homology to mouse is 84%. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_002417.2
Entrez基因汇总 Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. It is thought that the protein encoded by this gene is cleaved at both ends to yield the active enzyme, but this processing has not been fully described. The enzyme degrades soluble and insoluble elastin. It may play a role in aneurysm formation and studies in mice suggest a role in the development of emphysema. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. 基因符号 - HME
- MMP12
- MMP-12
- MME
- ME
纯化方法 Affinity Purfied UniProt编号 - P39900
UniProt汇总 FUNCTION: May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.
COFACTOR: Binds 4 calcium ions per subunit. & Binds 2 zinc ions per subunit.
SIZE: 470 amino acids; 54002 Da
TISSUE SPECIFICITY: Found in alveolar macrophages but not in peripheral blood monocytes.
DOMAIN: The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
SIMILARITY: Belongs to the peptidase M10A family. & Contains 4 hemopexin-like domains.产品使用声明 质量保证 Evaluated by Western Blot using a concentrated conditioned cell culture media collected from a proprietary human fibroblast cell line (Cat. No. AG902) and probing with 1 µg/mL of Anti-MMP-12.
Western Blot Analysis: 1 µg/ml of this antibody detected MMP-12 on 10 µg of lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
上一件merck millipore产品：merck millipore,默克密理博,MAB5276,Anti-Endorphin β Antibody, clone 3-E7
下一件merck millipore产品：merck millipore,默克密理博,71364,pRSF-2 Ek/LIC Vector Kit - Novagen
相关产品
merck millipore,默克密理博,SF-1591,CYBB; Human, Cyanine 3 RNA Detection Probe,SmartFlare
merck millipore,默克密理博,14-651M,MARK1 Protein, active, 250 µg
merck millipore,默克密理博,PS1014,PhosphoDetect™ Anti-JAK2 (pTyr1007/1008) Rabbit pAb
merck millipore,默克密理博,09-241,Anti-phospho JAK-2 (Tyr570) Antibody
merck millipore,默克密理博,MAB5276,Anti-Endorphin β Antibody, clone 3-E7
merck millipore,默克密理博,71364,pRSF-2 Ek/LIC Vector Kit - Novagen
![merck millipore,默克密理博,108602,N-[叁(羟甲)甲基]甘胺酸](/e/upload/m_pic/MM_watermark%5BMM_watermark-ALL%5D.jpg "merck millipore,默克密理博,108602,N-[叁(羟甲)甲基]甘胺酸")
merck millipore,默克密理博,108602,N-[叁(羟甲)甲基]甘胺酸
%5BABN208_IHC(P)-ALL%5D.jpg "merck millipore,默克密理博,ABN208,Anti-ARLIP6 Antibody")
merck millipore,默克密理博,ABN208,Anti-ARLIP6 Antibody
