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merck millipore,默克密理博,04-1530,Anti-MDM2 Antibody, clone 3G9
产品名称：Anti-MDM2 Antibody, clone 3G9
产品型号：04-1530
Anti-MDM2 Antibody, clone 3G9 is a Mouse Monoclonal Antibody for detection of MDM2 also known as Double minute 2 protein or Mdm2 p53 binding protein homolog & has been validated in WB, IP, ICC & IHC.
merck millipore,默克密理博,04-1530,Anti-MDM2 Antibody, clone 3G9
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M, H IHC, WB, ICC M Purified Monoclonal Antibody 描述 产品目录编号 04-1530 描述 Anti-MDM2 Antibody, clone 3G9 Alternate Names - ubiquitin-protein ligase E3 Mdm2
- Double minute 2 protein
- Mdm2 p53 binding protein homolog (mouse)
- Mdm2, transformed 3T3 cell double minute 2, p53 binding protein
- Mdm2, transformed 3T3 cell double minute 2, p53 binding protein (mouse)
- Oncoprotein Mdm2
- double minute 2, human homolog of
- p53-binding protein
- mouse double minute 2 homolog
- mouse double minute 2, human homolog of
- p53-binding protein Mdm2
背景信息 MDM2 is over-expressed in many tumors. Its principal function is the ubiquitination and degradation of p53 tumor suppressor protein. This monoclonal antibody also recognizes a peptide epitope around Thr-216 of murine MDM2 when Thr-216 is unphosphorylated. SMP14 also cross reacts with some cytokeratins (6, 14 & 16). This is only a problem when working with certain epithelial cells and not fibroblasts. 产品信息 格式 Purified 控制 - MCF-7 cell lysate
演示 Purified mouse monoclonal IgGκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. 应用 应用 Anti-MDM2 Antibody, clone 3G9 is a Mouse Monoclonal Antibody for detection of MDM2 also known as Double minute 2 protein or Mdm2 p53 binding protein homolog & has been validated in WB, IP, ICC & IHC. 主要应用 - Immunohistochemistry
- Western Blotting
- Immunocytochemistry
应用说明 Immunoprecipitation Analysis: A representative lot was used by an independent laboratory for this application. (Changgong, L., et al. (2002). Molecular and Cellular Biology. 22(21):7562-7571.) 生物信息 免疫原品种 His-tagged recombinant protein corresponding to human MDM2. 表位 Unknown 克隆 3G9 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 This antibody recognizes MDM2. 品种反应性 MouseHuman Species Reactivity Note Demonstrated to react with human. Predicted to react with mouse based on 100% sequence homology. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_002383.2
Entrez基因汇总 This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues. [provided by RefSeq]. 基因符号 - HDM2
- MDM2
- hdm2
- HDMX
- Hdm2
纯化方法 Protein G purfied UniProt编号 - Q00987
UniProt汇总 FUNCTION: Inhibits TP53/p53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Functions as a ubiquitin ligase E3, in the presence of E1 and E2, toward p53 and itself. Permits the nuclear export of p53 and targets it for proteasome-mediated proteolysis.
SIZE: 491 amino acids; 55233 Da
SUBUNIT: Binds p53, p73, ARF(P14), ribosomal protein L5 and specifically to RNA. Can interact also with retinoblastoma protein (RB), E1A-associated protein EP300 and the E2F1 transcription factor. Forms a ternary complex with p53 and WWOX. Interacts with USP7 and TBRG1. Isoform Mdm2-F does not interact with TP53/p53. Interacts with, and ubiquitinates HIV-1 Tat.
SUBCELLULAR LOCATION: Nucleus, nucleoplasm. Cytoplasm. Nucleus, nucleolus. Note=Expressed predominantly in the nucleoplasm. Interaction with ARF(P14) results in the localization of both proteins to the nucleolus. The nucleolar localization signals in both ARF(P14) and MDM2 may be necessary to allow efficient nucleolar localization of both proteins.
TISSUE SPECIFICITY: Ubiquitous. Isoform Mdm2-A, isoform Mdm2-B, isoform Mdm2-C, isoform Mdm2-D, isoform Mdm2-E, isoform Mdm2-F and isoform Mdm2-G are observed in a range of cancers but absent in normal tissues.
DOMAIN: Region I is sufficient for binding p53 and inhibiting its G1 arrest and apoptosis functions. It also binds p73 and E2F1. Region II contains most of a central acidic region required for interaction with ribosomal protein L5 and a putative C4-type zinc finger. The RING finger domain which coordinates two molecules of zinc interacts specifically with RNA whether or not zinc is present and mediates the hetero-oligomerization with MDM4. It is also essential for its ubiquitin ligase E3 activity toward p53 and itself.
PTM: Phosphorylated in response to ionizing radiation in an ATM- dependent manner.
DISEASE: Seems to be amplified in certain tumors (including soft tissue sarcomas, osteosarcomas and gliomas). A higher frequency of splice variants lacking p53 binding domain sequences was found in late-stage and high-grade ovarian and bladder carcinomas. Four of the splice variants show loss of p53 binding.
SIMILARITY: Belongs to the MDM2/MDM4 family. & Contains 1 RanBP2-type zinc finger. & Contains 1 RING-type zinc finger. & Contains 1 SWIB domain.
MISCELLANEOUS: MDM2 RING finger mutations that failed to ubiquitinate p53 in vitro did not target p53 for degradation when expressed in cells.产品使用声明 质量保证 Evaluated by Western Blot in MCF-7 cell lysate.
Western Blot Analysis: 0.5 µg/ml of this antibody detected MDM2 on 10 µg of MCF-7 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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