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品种反应性 主要应用 宿主 格式 抗体类型 H WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 07-672-I Replaces 04-386 描述 Anti-MYPT1 Antibody Alternate Names - Protein phosphatase myosin-binding subunit
- Protein phosphatase 1 regulatory subunit 12A
- 130 kDa myosin-binding subunit of smooth muscle myosin phosphatase
- Myosin phosphatase-targeting subunit 1
- Myosin phosphatase target subunit 1
- PP1M subunit M110
背景信息 Myosin phosphatase target subunit 1 (MYPT1), also called Myosin-binding subunit of Myosin phosphatase, is one of the subunits and an integral component of the Myosin phosphatase. Myosin phosphatase regulates the interaction of Actin and Myosin downstream of the guanosine triphosphatase Rho, which inhibits Myosin phosphatase through the action of Rho-kinase. It is also involved in smooth muscle contraction. Inhibition of Myosin light chain phosphatase results in Ca2+ sensitization of smooth muscle contraction. This inhibition is modulated through phosphorylation of MYPT1 by a ZIP-like kinase, which associates with MYPTI and phosphorylates the inhibitory site in smooth muscle. The phosphorylation of MYPT1 by protein kinase C results in altered dephosphoryation of Myosin by attenuating the binding of protein phosphatase 1 catalytic subunit (PP1c) and the phosphorylated 20 kDa Myosin light chain to MYPT1. PP1c interacts with at least four binding sites on the amino-terminus of MYPT1. MYPT1 is localized on stress fibers, and is distributed close to the cell membrane and at cell-cell contacts to regulate Myosin phosphatase activity. 产品信息 格式 Affinity Purified 演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Detect MYPT1 using this rabbit polyclonal antibody, Anti-MYPT1 Antibody validated for use in western blotting & IP. 主要应用 - Western Blotting
应用说明 Immunoprecipitation Analysis: 0.5µg from a representative lot immunoprecipitated MYPT1 from 0.5mg of HeLa cell lysate. 生物信息 免疫原品种 GST-tagged recombinant protein corresponding to the C-terminus of chicken MYPT1. 表位 C-terminus 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 品种反应性 Human Species Reactivity Note Human. Predicted to react with Chicken based on immunogen design. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_990454
基因符号 - MYPT1
- PPP1R12A
- MBS
纯化方法 Affinity purified UniProt编号 - Q90623
产品使用声明 质量保证 Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected MYPT1 in 10 µg of HeLa cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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下一件merck millipore产品：merck millipore,默克密理博,04-587,Anti-Cytokeratin 5 Antibody
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