merck millipore,默克密理博,07-2273,Anti-phospho-SPAK Antibody (Ser373) / phospho-OSR1 Antibody (Ser325)

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Merck Millipore 默克密理愽

品种反应性	主要应用	宿主	格式	抗体类型
Ca, H, M, R	WB	Rb	Affinity Purified	Polyclonal Antibody

描述
产品目录编号	07-2273
描述	Anti-phospho-SPAK Antibody (Ser373) / phospho-OSR1 Antibody (Ser325)
Alternate Names	Serine/threonine-protein kinase 39 DCHT
背景信息	As members of the (sterile 20)-like (STE20) kinase family, the oxidative-stress-responsive kinase 1 (OSR1) and the SPS1-related proline/alanine-rich kinase (SPAK) serve as key enzymes in a signaling cascade, which mediate the activity of Na+/K+/2Cl- cotransporters (NKCCs) in response to osmotic stress. Both kinases have a conserved carboxy-terminal (CCT) domain, which are 79% identical to each other at the sequence level, and are also highly conserved in orthologues of these enzymes in Caenorhabditis elegans, Drosophila, and Xenopus. The CCT domain reflects no similarity to sequences found in other proteins. This domain is known to recognize and interact with a unique peptide (Arg-Phe-Xaa-Val) motif, which is present in OSRT1- and SPAK-activating kinases (with-no-lysine-kinase 1 (WNK1 and WNK4) and also in STE20 kinase family substrates (NKCC1 and NKCC2). It is reported that phosphorylation of a Ser/Thr residue preceding the (Arg-Phe-Xaa-Val) motif results in steric clash, and subsequent dissociation from the CCT domain. Furthermore, patients suffering from Gordon syndrome carry mutated WNK1 and WNK4 genes.

产品信息
格式	Affinity Purified
控制	Hypotonic-stimulated HEK293 cells untreated and lambda phosphatase-treated.
演示	Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

应用
应用	This phospho-SPAK antibody is validated for use in WB for the detection of the phospho-SPAK protein.
主要应用	Western Blotting

生物信息
免疫原品种	KLH-conjugated linear peptide corresponding to human SPAK and OSR1 phosphorylated at Ser375 and Ser325, respectively.
表位	SPAK phosphorlyated at Ser373 and OSR1 phosphorlyated at Ser325.
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
特异性	This antibody recognizes SPAK phosphorlyated at Ser373 and OSR1 phosphorlyated at Ser325.
品种反应性	CanineHumanMouseRat
Species Reactivity Note	Demonstrated to react with Human and Mouse. Predicted to react with Rat and Canine based on 100% sequence homology.
抗体类型	Polyclonal Antibody
Entrez基因编号	NP_037365
Entrez基因汇总	This gene encodes a serine/threonine kinase that is thought to function in the cellular stress response pathway. The kinase is activated in response to hypotonic stress, leading to phosphorylation of several cation-chloride-coupled cotransporters. The catalytically active kinase specifically activates the p38 MAP kinase pathway, and its interaction with p38 decreases upon cellular stress, suggesting that this kinase may serve as an intermediate in the response to cellular stress.
基因符号	SPAK STK39
修改	Phosphorylation
纯化方法	Affinity Purified
UniProt编号	Q9UEW8
UniProt汇总	FUNCTION: May act as a mediator of stress-activated signals. SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note: Nucleus when caspase-cleaved.

产品使用声明
质量保证	Evaluated by Western Blot in hypotonic-stimulated HEK293 cells untreated and lambda phosphatase-treated. Western Blot Anlaysis: 0.5 µg/mL of this antibody detected SPAK / OSR1 in 10 µg hypotonic-stimulated HEK293 cells untreated and lambda phosphatase-treated.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at 2-8°C from date of receipt.

包装信息
数量	100 µg

merck millipore,默克密理博,07-2273,Anti-phospho-SPAK Antibody (Ser373) / phospho-OSR1 Antibody (Ser325)

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