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merck millipore,默克密理博,07-1014,Anti-Upf1 Antibody
产品名称：Anti-Upf1 Antibody
产品型号：07-1014
Anti-Upf1 Antibody is a rabbit polyclonal antibody for detection of Upf1 also known as ATP-dependent helicase RENT1 ,Nonsense mRNA reducing factor 1, Up-frameshift suppressor 1 homolog & has been vali More>>
merck millipore,默克密理博,07-1014,Anti-Upf1 Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Ca, B, H, M, R WB Rb Purified Polyclonal Antibody 描述 产品目录编号 07-1014 描述 Anti-Upf1 Antibody Alternate Names - yeast Upf1p homolog
- ATP-dependent helicase RENT1
- Nonsense mRNA reducing factor 1
- UP Frameshift 1
- UPF1 regulator of nonsense transcripts homolog (yeast)
- Up-frameshift suppressor 1 homolog
- delta helicase
- regulator of nonsense transcripts 1
- up-frameshift mutation 1 homolog
背景信息 Upf1 is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. Upf1 is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. 产品信息 格式 Purified 控制 - U2OS cell lysate
演示 Purified rabbit polyclonal in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide. 应用 应用 Anti-Upf1 Antibody is a rabbit polyclonal antibody for detection of Upf1 also known as ATP-dependent helicase RENT1 ,Nonsense mRNA reducing factor 1, Up-frameshift suppressor 1 homolog & has been validated in WB. 主要应用 - Western Blotting
生物信息 免疫原品种 KLH-conjugated synthetic linear peptide corresponding to Upf1. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody detects Upf1. 品种反应性 CanineBovineHumanMouseRat Species Reactivity Note Tested on human. Predicted to react with mouse, rat, bovine and canine based on sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_002902.2
Entrez基因汇总 This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene. [provided by RefSeq].
基因符号 - pNORF1
- EC 3.6.1.-
- FLJ43809
- FLJ46894
- HUPF1
- KIAA0221
- NORF1
- RENT1
- hUpf1
纯化方法 Affinity Purfied UniProt编号 - Q92900
UniProt汇总 FUNCTION: Part of a post-splicing multiprotein complex. Involved in nonsense-mediated decay (NMD) as part of the SMG1C complex, a mRNA surveillance complex that recognizes and degrades mRNAs containing premature translation termination codons (PTCs). The complex probably acts by associating with ribosomes during tranlation termination on mRNPs. If an exon junction complex (EJC) is located 50-55 or more nucleotides downstream from the termination codon, RENT1 is phosphorylated by SMG1, triggering nonsense-mediated decay (NMD). Essential for embryonic viability.
SUBUNIT STRUCTURE: Found in a complex with RENT1, RENT2, RENT3A and RENT3B. Found in a complex with PARN. Found in a post-splicing complex with SMG1, NXF1, RBM8A, RENT1, RENT2, RENT3A, RENT3B and RNPS1. Found in a mRNA decay complex with EXOSC2, EXOSC4, EXOSC10, PARN, XRN1, DCP2, RENT1, RENT2 and RENT3B. Interacts with EST1A and RENT2. Component of the SMG1C complex, at least composed of SMG1, SMG8 and SMG9. The SMG1C complex is then recruited on premature translation termination codons (PTCs) to form the ribosome:SURF complex, at least composed of ERF1, ERF3 (ERF3A or ERF3B), EEF2, UPF1/RENT1, SMG1, SMG8 and SMG9. Interacts (when hyperphosphorylated) with PNRC2.
SUBCELLULAR LOCATION: Cytoplasm. Cytoplasm › P-body. Note: Hyperphosphorylated form is targeted to the P-body, while unphosphorylated protein is distributed throughout the cytoplasm.
TISSUE SPECIFICITY: Ubiquitous.
DOMAIN: The [ST]-Q motif constitutes a recognition sequence for kinases from the PI3/PI4-kinase family.
PTM: Phosphorylated by SMG1; required for formation of mRNA surveillance complexes. Phosphorylated upon DNA damage, probably by ATM or ATR.
SEQUENCE SIMILARITIES: Belongs to the DNA2/NAM7 helicase family.
Contains 1 C2H2-type zinc finger.
产品使用声明 质量保证 Evaluated by western blot on U2OS cell lysate.
Western Blot Analysis: 0.5-2 µg/mL of this antibody detected Upf1 in U2OS cell lysate.
使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8ºC from date of receipt. 包装信息 数量 100 µL
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