merck millipore,默克密理博,APT115,Apo-BrdU™ Detection Kit

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merck millipore,默克密理博,APT115,Apo-BrdU™ Detection Kit
产品名称：Apo-BrdU™ Detection Kit
产品型号：APT115
The kit measures apoptosis in cells.

merck millipore,默克密理博,APT115,Apo-BrdU™ Detection Kit

产品介绍

merck millipore,默克密理博,APT115,Apo-BrdU™ Detection Kit

重要规格表

主要应用	检测方法
FC	Fluorescent

描述
产品目录编号	APT115
品牌系列	Chemicon®
商名	Chemicon Apo-BrdU
描述	Apo-BrdU™ Detection Kit
概述	The CHEMICON APO-BRDU™ Kit is a two color staining method for labeling DNA breaks and total cellular DNA to detect apoptotic cells by flow cytometry (Li et al. 1995). The kit contains the instructions and reagents required for measuring apoptosis in cells including; positive and negative control cells for assessing reagent performance; washing, reaction, and rinsing buffers for processing individual steps in the assay; terminal deoxynucleotidyl tranferase enzyme (TdT), bromodeoxyuridine triphosphate (Br-dUTP), and fluorescein labeled anti-BrdU antibody for labeling DNA breaks and Propidium Iodide/RNase A solution for counter staining the total DNA.

产品信息
部件	PI/RNase Staining Buffer, Amber bottle The APO-BRDU™ Kit is shipped in one container and consists of two packages. One package is shipped at ambient temperature and should be stored at 2-8°C upon arrival. The other package is styrofoam containing frozen ice packs and the reagent contents should be stored at -20°C upon arrival. Note: Upon arrival store the reagents at the appropriate temperatures. Reagent bottles have color coded caps to aid in their identification. Sufficient reagents are provided to process 60 cell suspensions including 5 mL positive and 5 mL negative control cell suspensions of approximately 1 x 106 cells per mL in 70% (v/v) ethanol. The control cells are derived from a human lymphoma cell line and have been fixed as described on page 6. Positive Control Cells, Brown cap Negative Control Cells, White cap Wash Buffer, Blue cap Reaction Buffer, Green cap TdT Enzyme, Yellow cap Br-dUTP, Violet cap Rinsing Buffer, Red cap Fluorescein~PRB-1 mAb, Orange cap
检测方法	Fluorescent

产品信息

部件

PI/RNase Staining Buffer, Amber bottle
The APO-BRDU™ Kit is shipped in one container and consists of two packages. One package is shipped at ambient temperature and should be stored at 2-8°C upon arrival. The other package is styrofoam containing frozen ice packs and the reagent contents should be stored at -20°C upon arrival. Note: Upon arrival store the reagents at the appropriate temperatures.
Reagent bottles have color coded caps to aid in their identification. Sufficient reagents are provided to process 60 cell suspensions including 5 mL positive and 5 mL negative control cell suspensions of approximately 1 x 106 cells per mL in 70% (v/v) ethanol. The control cells are derived from a human lymphoma cell line and have been fixed as described on page 6.
Positive Control Cells, Brown cap
Negative Control Cells, White cap
Wash Buffer, Blue cap
Reaction Buffer, Green cap
TdT Enzyme, Yellow cap
Br-dUTP, Violet cap
Rinsing Buffer, Red cap
Fluorescein~PRB-1 mAb, Orange cap

检测方法

Fluorescent

应用
应用	The kit measures apoptosis in cells.
主要应用	Flow Cytometry

生物信息
品种反应性	All

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Precautions and Warnings 1. The components of this kit are for Research Use only and are not intended for diagnostic procedures. 2. Positive and negative control cells contain 70% (v/v) ethanol as a preservative; wash and reaction buffer contain sodium cacodylate (dimethylarsinic) as a buffer; rinsing and PI/RNase staining buffer contain 0.05% (w/v) sodium azide as a preservative. These materials are harmful if swallowed; avoid skin contact, wash immediately with water. See Material Safety Data Sheets. 3. TdT Enzyme will not freeze at -20oC, because it is in 50% (v/v) glycerol solution. Upon warming the TdT enzyme solution, centrifuge the tube for 30 seconds to force all the liquid to the bottom of the tube.

储存和货运信息

存储条件

Precautions and Warnings

1. The components of this kit are for Research Use only and are not intended for diagnostic procedures.

2. Positive and negative control cells contain 70% (v/v) ethanol as a preservative; wash and reaction buffer contain sodium cacodylate (dimethylarsinic) as a buffer; rinsing and PI/RNase staining buffer contain 0.05% (w/v) sodium azide as a preservative. These materials are harmful if swallowed; avoid skin contact, wash immediately with water. See Material Safety Data Sheets.

3. TdT Enzyme will not freeze at -20oC, because it is in 50% (v/v) glycerol solution. Upon warming the TdT enzyme solution, centrifuge the tube for 30 seconds to force all the liquid to the bottom of the tube.