merck millipore,默克密理愽
  • merck millipore,默克密理博,07-2184,Anti-acetyl-GAPDH (Lys160) Antibody

  • merck millipore,默克密理博,07-2184,Anti-acetyl-GAPDH (Lys160) Antibody

    产品名称：Anti-acetyl-GAPDH (Lys160) Antibody
    产品型号：07-2184
    This Anti-acetyl-GAPDH (Lys160) antibody is validated for use in western blotting, peptide inhibition assay & IP for the detection of acetyl-GAPDH (Lys160).

    merck millipore,默克密理博,07-2184,Anti-acetyl-GAPDH (Lys160) Antibody

  • 产品介绍
  • merck millipore,默克密理博,07-2184,Anti-acetyl-GAPDH (Lys160) Antibody

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    HWBRbAffinity PurifiedPolyclonal Antibody
    描述
    产品目录编号 07-2184
    描述 Anti-acetyl-GAPDH (Lys160) Antibody
    Alternate Names
    • Peptidyl-cysteine S-nitrosylase GAPDH
    • GAPDH
    • Glyceraldehyde-3-phosphate dehydrogenase
    背景信息 Acetyl-GAPDH (Lys160), also known as Glyceraldehyde-3-phosphate dehydrogenase (GAPDH(lys160), or Peptidyl-cysteine S-nitrosylase GAPDH, and encoded by the gene GAPDH/GAPD, is an interesting multifunctional metabolic enzyme that plays a critical role in glycolysis but also many other important nuclear and cell functions such as transcription, RNA transport, DNA replication and even apoptosis and cell death. In glycolysis, GAPDH is a key enzyme that catalyzes the first step in the pathway by converting G3P into 3-phospho-D glyceroyl phosphate. An example of GAPDH’s regulation of gene transcription as a component of a GAIT complex, which selectively inhibits the activation of various gamma interferon induced inflammation responses. The protein-protein interactions of GAPDH are at least partially controlled by acetylation. For instance, transport of GAPDH into the nucleus is dependent upon acetylation at lysines 117, 227, and 251, and acetylation at lysine 183 governs co-factor interactions. Acetylation of GAPDH at lysine 160 ultimately is involved in p53 mediated cell death.
    产品信息
    格式 Affinity Purified
    演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
    应用
    应用 This Anti-acetyl-GAPDH (Lys160) antibody is validated for use in western blotting, peptide inhibition assay & IP for the detection of acetyl-GAPDH (Lys160).
    主要应用
    • Western Blotting
    应用说明 Peptide Inhibition Analysis: 1 µg/mL from a representative lot detected acetyl GAPDH (Lys60) in LPS/IFNgamma in RAW264.7 nuclear extract.

    Immunoprecipitation Analyis: A representative lot immunoprecipitated acetyl GAPDH (Lys60) from LPS/IFNgamma treated RAW264.7 cell lysate (Prof. Akira Sawa, Johns Hopkins University, Baltimore.).
    生物信息
    免疫原品种 KLH-conjugated linear peptide corresponding to the human GAPDH acetylated at Lys160.
    表位 0
    浓缩 0.5 mg/mL
    宿主 Rabbit
    品种反应性 Human
    Species Reactivity Note Human. Predicted to react with Mouse, Rat, Non-Human Primate, Bovine, Horse, Feline, Sheep, Porcine, and Canine based on 100% sequence homology.
    抗体类型 Polyclonal Antibody
    Entrez基因编号
    • NP_002037
    基因符号
    • GAPD
    • GAPDH
    修改
    • Acetylation
    纯化方法 Affinity purified
    UniProt编号
    • P04406
    产品使用声明
    质量保证 Evaluated by Western Blotting in untreated and LPS/IFNgamma treated RAW264.7 cell lysate.

    Western Blotting Analysis: 1 µg/mL of this antibody detected acetyl GAPDH (Lys60) in LPS/IFNgamma treated RAW264.7 cell lysate and not in untreated RAW264.7 cell lysate.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 1 year at 2-8°C from date of receipt.
    包装信息
    数量 100 µg

    merck millipore,默克密理博,07-2184,Anti-acetyl-GAPDH (Lys160) Antibody

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