merck millipore,默克密理博,MAB1596,Anti-Post Synaptic Density Protein 95 Antibody, clone 6G6-1C9

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merck millipore,默克密理博,MAB1596,Anti-Post Synaptic Density Protein 95 Antibody, clone 6G6-1C9
产品名称：Anti-Post Synaptic Density Protein 95 Antibody, clone 6G6-1C9
产品型号：MAB1596
Anti-Post Synaptic Density Protein 95 Antibody, clone 6G6-1C9 detects level of Post Synaptic Density Protein 95 & has been published & validated for use in IC, IH & WB.

merck millipore,默克密理博,MAB1596,Anti-Post Synaptic Density Protein 95 Antibody, clone 6G6-1C9

产品介绍

merck millipore,默克密理博,MAB1596,Anti-Post Synaptic Density Protein 95 Antibody, clone 6G6-1C9

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, B, M	WB, ICC, IHC	M	Purified	Monoclonal Antibody

描述
产品目录编号	MAB1596
Replaces	04-1066
品牌系列	Chemicon®
商名	Chemicon
描述	Anti-Post Synaptic Density Protein 95 Antibody, clone 6G6-1C9
Alternate Names	PSD-95
背景信息	PSD-95 is part of a multiprotein complex restricted to the post-synaptic membrane. It is believed to initiate NMDA receptor clustering on the post synaptic membrane. Its restricted expression makes it an excellent marker for post-synaptic membrane regions on neurons.

产品信息
格式	Purified
控制	Mouse brain, rat brain tissue.
演示	Purified immunoglobulin. Liquid in PBS, pH 7.2 with 50% glycerol and 0.09% sodium azide.

应用
应用	Anti-Post Synaptic Density Protein 95 Antibody, clone 6G6-1C9 detects level of Post Synaptic Density Protein 95 & has been published & validated for use in IC, IH & WB.
主要应用	Western Blotting Immunocytochemistry Immunohistochemistry
应用说明	Immunocytochemistry: Representative image from a previous lot. Confocal fluorescent analysis of PC12 using MAb1596 Mouse mAB (Red). Actin filaments have been labeled with Alexa Fluor 488 -Phalloidin (Green). Nuclear is stained with DAPI (Blue). Membrane staining positive. Immunocytochemistry: 12.5 µg/mL Western Blot: 1-5 μg/mL (ECL) on rat brain lysate (20 μg). Optimal working dilutions must be determined by the end user.

生物信息
免疫原品种	Recombinant rat PSD-95.
克隆	6G6-1C9
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Mouse
特异性	Reacts with a protein of ~100 kDa, corresponding to the apparent molecular weight of PSD-95, on SDS-PAGE immunoblots of samples from rat, mouse and bovine. A slightly higher molecular weight band is also detected. In rat brain and mouse brain tissue extracts a band of ~75 kDa is also present.
同种型	IgG2a
品种反应性	RatBovineMouse
Species Reactivity Note	Rat, mouse and bovine. Other species have not been tested.
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_001128827.1
Entrez基因汇总	This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family. It heteromultimerizes with another MAGUK protein, DLG2, and is recruited into NMDA receptor and potassium channel clusters. These two MAGUK proteins may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors, ion channels, and associated signaling proteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
基因符号	PSD-95
纯化方法	See datasheet for purification method.
UniProt编号	P78352
UniProt汇总	FUNCTION: SwissProt: P78352 # Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ACCN3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B (By similarity). SIZE: 724 amino acids; 80495 Da SUBUNIT: Interacts through its first two PDZ domains with GRIN2A, GRIN2B, GRIN2C, GRIN2D, ACCN3, certain splice forms of GRIN1, KCND2, CXADR and SYNGAP1. Interacts through its second PDZ domain with the PDZ domain of NOS1 or the C-terminus of CAPON. May interact with HTR2A. Interacts through its guanylate kinase-like domain with DLGAP1/GKAP, DLGAP2, DLGAP3, DLGAP4, MAP1A, and BEGAIN (By similarity). Interacts through its first two PDZ domains with KCNA1, KCNA2, KCNA3, KCNA4 and ERBB4. Interacts through its first PDZ domain with GRIK2, KCNA4 and CRIPT. Interacts through its third PDZ domain with NLGN1, and probably with NLGN2 and NLGN3. Interacts through its guanylate kinase-like domain with KIF13B. Isoform 2 interacts through an L27 domain with HGS/HRS and the first L27 domain of CASK. SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell junction, synapse. Note=High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells. TISSUE SPECIFICITY: Brain. DOMAIN: SwissProt: P78352 The PDZ domain 3 mediates interaction with ADR1B. & The L27 domain near the N-terminus of isoform 2 is required for HGS/HRS-dependent targeting to post-synaptic density. PTM: Palmitoylation of isoform 1 is required for targeting to postsynaptic density (By similarity). SIMILARITY: Belongs to the MAGUK family. & Contains 1 guanylate kinase-like domain. & Contains 3 PDZ (DHR) domains. & Contains 1 SH3 domain.

产品使用声明
质量保证	Routinely evaluated in Immunocytochemistry.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 6 months at -20°C in undiluted aliquots from date of receipt. Do not store below -20°C. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 6 months at -20°C in undiluted aliquots from date of receipt. Do not store below -20°C.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.