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品种反应性 主要应用 宿主 格式 抗体类型 Eq, M, R, H, B, Ch WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 07-1643 描述 Anti-phospho-Akt (Tyr450) Antibody Alternate Names - v-akt murine thymoma viral oncogene homolog 1
- AKT1 kinase
- Protein kinase B
- RAC-alpha serine/threonine-protein kinase
- rac protein kinase alpha
背景信息 Akt (protein kinase B), a serine/threonine kinase, has emerged as a critical enzyme in signal transduction pathways involved in cell proliferation, apoptosis, angiogenesis, and diabetes. In mammals three isoforms of Akt (α, β, γ or Akt 1, 2, 3) are reported that exhibit a high degree of homology, but differ slightly in the localization of their regulatory phosphorylation sites. Akt α is the predominant isoform in most tissues, whereas the highest expression of Akt β is observed in the insulin-responsive tissues, and Akt γ is abundant in brain tissue. Each Akt isoform is composed of three functionally distinct regions: an N-terminal pleckstrin homology (PH) domain that provides a lipid-binding module to direct Akt to PIP2 and PIP3, a central catalytic domain, and a C-terminal hydrophobic motif. 产品信息 格式 Affinity Purified 控制 - Lambda phosphatase treated and untreated NIH/3T3 with Anisomycin, RAT2 with SRC, and MCF7 with EGF
演示 Affinity purified rabbit serum in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. 应用 应用 Anti-phospho-Akt (Tyr450) Antibody is an antibody against phospho-Akt (Tyr450) for use in WB, EA. 主要应用 - Western Blotting
应用说明 Western Blot (SNAP ID) Analysis: 1:1,000 dilution from a previous lot detected AKT phosphorylated at Tyr450 in NIH/3T3 cell lysate. 生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to human phosphorylated AKT at and around Tyr450. 表位 Phosphorylated Tyr450 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes phosphorylated Akt at Tyr450. 品种反应性 HorseMouseRatHumanBovineChicken Species Reactivity Note Reacts with human, mouse, and rat. Predicted to react with cow, chicken, and horse based on sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_005154
Entrez基因汇总 The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq] 基因符号 - AKT
- AKT1
- RAC
- PRKBA
- RAC-ALPHA
- RAC-PK-alpha
- C-AKT
- PKB
修改 - Phosphorylation
纯化方法 Affinity Purfied UniProt编号 - P31749
UniProt汇总 FUNCTION: General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis, partly by playing a role in both insulin-induced phosphorylation of 4E-BP1 and in insulin-induced activation of p70 S6 kinase. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.
SIZE: 480 amino acids; 55686 Da
SUBUNIT: Interacts with CENTG1 isoform 2 (PIKE-A) in the presence of guanine nucleotides. The C-terminus interacts with CCDC88A/GRDN. Interacts with AKTIP.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Nucleus after activation by integrin-linked protein kinase 1 (ILK1).
TISSUE SPECIFICITY: In all human cell types so far analyzed.
DOMAIN: Binding of the PH domain to the phosphatidylinositol 3- kinase alpha (PI(3)K) results in its targeting to the plasma membrane.
PTM: Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Ser-473 phosphorylation by the Rictor-mTor complex favors Thr-308 phosphorylation by PDPK1. Ser-473 phosphorylation is enhanced by interaction with CENTG1 isoform 2 (PIKE-A).
SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 PH domain. & Contains 1 protein kinase domain.产品使用声明 质量保证 Evaluated by Western Blot in NIH/3T3, RAT2, and MCF7 cell lysates.
Western Blot Analysis: 1:250-1,000 dilution of this antibody detected AKT phosphorylated at Tyr450 in NIH/3T3, RAT2, and MCF7 cell lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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