merck millipore,默克密理博,04-581,Anti-Paxillin (NT) Antibody, rabbit monoclonal, clone Y113

搜索"Merck Millipore 密理愽"产品

Merck Millipore 默克密理愽

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	ICC, WB	Rb	Purified	Monoclonal Antibody

描述
产品目录编号	04-581
品牌系列	Upstate
商名	Upstate
描述	Anti-Paxillin (NT) Antibody, rabbit monoclonal, clone Y113
背景信息	Paxillin is a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion) (1). Paxillin plays a significant role in integrin signaling and the tyrosine phosphorylation of paxillin is required for integrin-mediated cytoskeletal reorganization (2). Paxillin is phosphorylated by focal adhesion kinase (FAK) at Tyr118 (3). It has been found that phosphorylation of paxillin at both Tyr118 and Tyr31 is essential for MM1 cancer cell migration (4).

产品信息
格式	Purified
演示	Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. This Anti-Paxillin (N-Term) Antibody, Rabbit, clone Y113 is validated for use in IP, WB, IC, IH(P) for the detection of Paxillin (N-Term).
主要应用	Immunocytochemistry Western Blotting
应用说明	WB: 1:5,000-10,000 IHC: 1:100 (Paraffin-embedded breast carcinoma) ICC: 1:250 (Hela cells) IP: 1:200

生物信息
免疫原品种	A synthetic peptide corresponding to residues in N-terminus of human Paxillin. This antibody detects alpha, beta and gamma isoforms of Paxillin.
克隆	Y113
宿主	Rabbit
特异性	Recognizes Human, Mouse, Rat Paxillin (N-Term).
品种反应性	RatHumanMouse
Species Reactivity Note	Positive control: Hela cell lysate
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_002859.1
基因符号	FLJ16691 PXN Paxillin. paxillin
UniProt编号	P49023
UniProt汇总	FUNCTION: SwissProt: P49023 # Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). SIZE: 591 amino acids; 64533 Da SUBUNIT: Binds in vitro to vinculin as well as to the SH3 domain of c-SRC and, when tyrosine phosphorylated, to the SH2 domain of V-CRK. Isoform beta binds to focal adhesion kinase but weakly to vinculin. Isoform gamma binds to vinculin but only weakly to focal adhesion kinase. Interacts with GIT1, NUDT16L1/SDOS, PARVA and TGFB1I1. Component of cytoplasmic complexes, which also contain GIT1, ARHGEF6 and PAK1 (By similarity). Binds DDEF2. Interacts with unphosphorylated ITGA4. Interacts with RNF5. PTM: Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens. SIMILARITY: SwissProt: P49023 ## Belongs to the paxillin family. & Contains 4 LIM zinc-binding domains.

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Store at -20 °C. Stable for 12 months from date of receipt.

包装信息
数量	100 µL

merck millipore,默克密理博,04-581,Anti-Paxillin (NT) Antibody, rabbit monoclonal, clone Y113

whatman,沃特曼,nalgene,耐洁,merck millipore,默克密理愽,sartorius,赛多利斯,pall,颇尔
thermo fisher,赛默飞世尔,GE,MN,macherey-nagel,advantec,wheaton,brand,普兰德,welch,airtech