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merck millipore,默克密理博,MABT38,Anti-Laminin subunit alpha-4 Antibody, clone 3H2
产品名称：Anti-Laminin subunit alpha-4 Antibody, clone 3H2
产品型号：MABT38
Anti-Laminin subunit alpha-4 Antibody, clone 3H2 detects level of Laminin subunit alpha-4 & has been published & validated for use in WB, IH, IP, ELISA.
merck millipore,默克密理博,MABT38,Anti-Laminin subunit alpha-4 Antibody, clone 3H2
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H ELISA, WB, IHC M Purified Monoclonal Antibody 描述 产品目录编号 MABT38 描述 Anti-Laminin subunit alpha-4 Antibody, clone 3H2 Alternate Names - Laminin-14 subunit alpha
- laminin, alpha 4
- laminin subunit alpha-4
- Laminin-8 subunit alpha
- Laminin-9 subunit alpha
- laminin alpha 4 chain
背景信息 Laminins are basement membrane, extracellular matrix glycoproteins that are composed of alpha, beta and gamma heterotrimeric chains. Different combinations of five alpha chains, three beta chains, and two gamma chains form at least 12 laminin isoforms. Laminin subunit alpha-4 contains the C-terminal G domain, and although implicated in developmental regulation due to expression profiles, the biological function of laminin subunit alpha-4 is yet to be fully understood. Laminis as a whole play an important role in cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. High expression has been observed in adult heart, lung, ovary, small and large intestines, placenta, and liver, with lower levels observed in skeletal muscle, kidney, pancreas, testis, prostate, and rain. 产品信息 格式 Purified 控制 - Human serum tissue lysate (non-reduced)
演示 Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-Laminin subunit alpha-4 Antibody, clone 3H2 detects level of Laminin subunit alpha-4 & has been published & validated for use in WB, IH, IP, ELISA. 主要应用 - ELISA
- Western Blotting
- Immunohistochemistry
应用说明 Immunohistochemistry Analysis: A 1:500 dilution from a previous lot detected Laminin subunit alpha-4 in basal cell carcinoma tissue.
ELISA Analysis: A previous lot was used by an independent laboratory in ELISA. (Wondimu, Z., et al. (2004). Blood. 104(6):1859-1866.)
Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in IP. (Wondimu, Z., et al. (2004). Blood. 104(6):1859-1866.)生物信息 免疫原品种 Recombinant protein corresponding to human Laminin subunit alpha-4. 克隆 3H2 宿主 Mouse 同种型 IgG1κ 品种反应性 Human Species Reactivity Note Demonstrated to react with human. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_001098676
Entrez基因汇总 Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the alpha chain isoform laminin, alpha 4. The domain structure of alpha 4 is similar to that of alpha 3, both of which resemble truncated versions of alpha 1 and alpha 2, in that approximately 1,200 residues at the N-terminus (domains IV, V and VI) have been lost. Laminin, alpha 4 contains the C-terminal G domain which distinguishes all alpha chains from the beta and gamma chains. The RNA analysis from adult and fetal tissues revealed developmental regulation of expression, however, the exact function of laminin, alpha 4 is not known. Tissue-specific utilization of alternative polyA-signal has been described in literature. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq]. 基因符号 - LAMA4
- LAMA3
纯化方法 Protein G Purified UniProt编号 - Q16363
UniProt汇总 FUUNCTION: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
SUBUNIT STRUCTURE: Laminin is a complex glycoprotein, consisting of three different polypeptide chains (alpha, beta, gamma), which are bound to each other by disulfide bonds into a cross-shaped molecule comprising one long and three short arms with globules at each end. Alpha-4 is a subunit of laminin-8 (laminin-411), laminin-9 (laminin-421) and laminin-14 (laminin-423).
SUBCELLLULAR LOCATION: Secreted › extracellular space › extracellular matrix › basement membrane. Note: Major component.
TISSUE SPECIFICTY: In adult, strong expression in heart, lung, ovary small and large intestines, placenta, liver; weak or no expression in skeletal muscle, kidney, pancreas, testis, prostate, brain. High expression in fetal lung and kidney. Expression in fetal and newborn tissues is observed in certain mesenchymal cells in tissues such as smooth muscle and dermis.
DOMAIN: The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
Domain G is globular.
SEQUENCE SIMILARITIES: Contains 4 laminin EGF-like domains.
Contains 5 laminin G-like domains.
CAUTION: Gene LAMA4 was formerly called LAMA3.
SEQUENCE CAUTION: The sequence BAE06109.1 differs from that shown. Reason: Erroneous initiation.产品使用声明 质量保证 Evaluated by Western Blot in human serum tissue lysate under non-reduced conditions.
Western Blot Analysis: A 1:50,000 dilution of this antibody detected Laminin subunit alpha-4 in 10 µg of human serum tissue lysate (non-reduced conditions).使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µL
上一件merck millipore产品：merck millipore,默克密理博,324518,eIF4E/eIF4G Interaction Inhibitor II, 4E1RCat - Calbiochem
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