merck millipore,默克密理博,AB10022,Anti-Histone H2A.X Antibody

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Merck Millipore 默克密理愽

品种反应性	主要应用	宿主	格式	抗体类型
B, H, M, R	FC, WB	Rb	Affinity Purified	Polyclonal Antibody

描述
产品目录编号	AB10022
品牌系列	Upstate
商名	Upstate
描述	Anti-Histone H2A.X Antibody
Alternate Names	H2AFX Histone H2A.X Histone H2AX H2a/x
背景信息	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. H2A.X, also known as H2AFX, is a variant of Histone H2A that is found in a subset of nucleosomes. H2A.X has a unique C-terminal tail that becomes phosphorylated following double-stranded DNA breaks following irradiation or apoptosis. H2AX is involved in DNA repair and the maintenance of genomic stability. It is been implicated both in homologous recombination and nonhomologous end joining DNA repair pathways. H2A.X is thought to have a critical function in the recruitment of DNA repair factors and DNA damage-signaling proteins. H2A.X is ubiquitinated by RING1 and RNF2/RING2 complex providing a specific mark for transcriptional repression. In addition, this protein can be ubiquitinated by the E2 ligase UBE2N and the E3 ligases RNF8 and RNF168 as result of double-stranded DNA breaks to recruit repair proteins at a damaged site.

产品信息
格式	Affinity Purified
演示	Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

应用
应用	Use Anti-Histone H2A.X Antibody, Human (Rabbit Polyclonal Antibody) validated in WB to detect Histone H2A.X also known as H2AFX.
主要应用	Flow Cytometry Western Blotting
应用说明	Western Blotting Analysis: A 1:10,000 dilution of this antibody detected 500 ng of recombinant Histone H2A.X. Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Histone H2A.X in 10 µg of Rat2 acid extract, BALB 3T3/A31 acid extract, and calf thymus histone extract tissue lysate. Flow Cytometry Analysis: 1.0 µg of this antibody from a representative lot detected Histone H2A.X in 1X10E6 HeLa cells.

生物信息
免疫原品种	KLH-conjugated linear peptide corresponding to the C-terminus of human Histone H2A.X.
表位	a.a. 121-139
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
品种反应性	BovineHumanMouseRat
Species Reactivity Note	Human, Mouse, Rat, Bovine.
抗体类型	Polyclonal Antibody
Entrez基因编号	NP_002096
基因符号	H2AX H2AFX
纯化方法	Peptide affinity chromatography
UniProt编号	P16104

产品使用声明
质量保证	Evaluated by Western Blotting in HeLa acid extract. Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Histone H2A.X in 10 µg of HeLa acid extract.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at 2-8°C from date of receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

包装信息
数量	100 µL

merck millipore,默克密理博,AB10022,Anti-Histone H2A.X Antibody

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