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merck millipore,默克密理博,07-1596,Anti-SIRT3 (CT) Antibody
产品名称：Anti-SIRT3 (CT) Antibody
产品型号：07-1596
Anti-SIRT3 (C-terminus) Antibody is a Rabbit Polyclonal Antibody for detection of SIRT3 (C-terminus) also known sirtuin 3 & has been validated in WB, ELISA & IHC.
merck millipore,默克密理博,07-1596,Anti-SIRT3 (CT) Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H, M IHC, WB, ELISA Rb Purified Polyclonal Antibody 描述 产品目录编号 07-1596 描述 Anti-SIRT3 (CT) Antibody Alternate Names - sirtuin type 3
- SIR2-like protein 3
- mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase
- silent mating type information regulation 2, S.cerevisiae, homolog 3
- sir2-like 3
- sirtuin (silent mating type information regulation 2<br />homolog) 3 (S. cerevisiae)
- sirtuin (silent mating type information regulation 2,<br />S.cerevisiae, homolog) 3
- sirtuin 3
背景信息 SIRT3 is a member of the class I sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. SIRT3 is located in the mitochondria and has been implicated in regulating metabolic processes. Endogenous SIRT3 is a soluble protein located in the mitochondrial matrix. Overexpression of Sirt3 in cultured cells increases respiration and decreases the production of reactive oxygen species. Fasting increases Sirt3 expression in white and brown adipose tissue. A longer form of SIRT3 is present in the nucleus where it exhibits histone deacetylase activity. SIRT3 protects cardiomyocytes from stress mediated cell death due to deacetylation of a nuclear factor, Ku-70. 产品信息 格式 Purified 控制 - C2C12 cell lysate
演示 Purified rabbit polyclonal in buffer containing 0.09% NaN3. 应用 应用 Anti-SIRT3 (C-terminus) Antibody is a Rabbit Polyclonal Antibody for detection of SIRT3 (C-terminus) also known sirtuin 3 & has been validated in WB, ELISA & IHC. 主要应用 - Immunohistochemistry
- Western Blotting
- ELISA
应用说明 ELISA:
This antibody has been shown by an outside laboratory to be suitable for ELISA (1:1000).
Immunohistochemistry:
This antibody has been shown by an outside laboratory to be suitable for histochemistry using human hepatocarcinoma tissue (1:50 – 1:100).
生物信息 免疫原品种 The immunogen was a peptide corresponding the C-terminal region of human SIRT3. 表位 C-terminus 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes SIRT3. Endogenous levels of this protein vary among cell lines; the protein was easily detected in RAW264.7 and C2C12 cells but only weakly detected in HeLa cells, and not detected in Jurkat, HEK293 or PC3 cells. 品种反应性 HumanMouse Species Reactivity Note Human, Mouse. Not tested in other species. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_036371
Entrez基因汇总 This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two alternatively spliced transcript variants that encode different proteins have been described for this gene. [provided by RefSeq].
基因符号 - EC 3.5.1
- SIR2L3
- hSIRT3
纯化方法 Purified UniProt编号 - Q9NTG7
UniProt汇总 FUNCTION:NAD-dependent deacetylase. Despite some ability to deacetylate histones in vitro, it is unlikely in vivo.
Catalytic activity NAD+ + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein. Ref.4 Ref.5
Cofactor Binds 1 zinc ion per subunit By similarity.
SUBCELLULAR LOCATION:Mitochondrion matrix. Ref.4 Ref.5
TISSUE SPECIFICITY: Widely expressed. Ref.5 Ref.1
PTM: Processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product. Such processing is probably essential for its enzymatic activity.
SEQUENCE SIMILARITY: Belongs to the sirtuin family.
Contains 1 deacetylase sirtuin-type domain.
产品使用声明 质量保证 Western Blot Analysis:
1:250 dilution of this antibody detected SIRT3 on 10 µg of C2C12 lysate.
使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 6 months at 2-8°C from date of receipt. For long term storage, store at -20ºC. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. 包装信息 数量 100 µg
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