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merck millipore,默克密理博,07-1586,Anti-HIF-1-alpha Antibody, hydroxyproline (Pro564)
产品名称：Anti-HIF-1-alpha Antibody, hydroxyproline (Pro564)
产品型号：07-1586
Anti-HIF-1-alpha Antibody, hydroxyproline (Pro564) is a rabbit polyclonal antibody for detection of HIF-1-alpha also known as Hypoxia-inducible factor 1-alpha, HIF1-alpha & has been validated in WB, P More>>
merck millipore,默克密理博,07-1586,Anti-HIF-1-alpha Antibody, hydroxyproline (Pro564)
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 07-1586 描述 Anti-HIF-1-alpha Antibody, hydroxyproline (Pro564) Alternate Names - PAS domain-containing protein 8
- Hypoxia-inducible factor 1-alpha
- HIF-1-alpha
- HIF1-alpha
- ARNT-interacting protein
- Basic-helix-loop-helix-PAS protein
- MOP1
- Class E basic helix-loop-helix protein 78
- bHLHe78
- Member of PAS protein 1
背景信息 Cell growth and viability is compromised by oxygen deprivation (hypoxia). Hypoxia-inducible factors, including HIF-1α, HIF-1β (also designated Arnt 1), EPAS-1 (also designated HIF-2α) and HIF-3α, induce glycolysis, erythropoiesis and angiogenesis in order to restore oxygen homeostasis. Hypoxia-inducible factors are members of the Per-Arnt-Sim (PAS) domain transcription factor family. In response to hypoxia, HIF-1α is upregulated and forms a heterodimer with HIF-1β to form the HIF-1 complex. The HIF-1 complex recognizes and binds to the hypoxia responsive element (HRE) of hypoxia-inducible genes, thereby activating transcription. Hypoxia-inducible expression of some genes such as Glut-1, p53, p21 or Bcl-2, is HIF-1α dependent, whereas expression of others, such as p27, GADD 153 or HO-1, is HIF-1α independent. EPAS-1 and HIF-3α have also been shown to form heterodimeric complexes with Arnt 1 in response to hypoxia. 产品信息 格式 Affinity Purified 控制 - In vitro transcribed and translated HIF-1-alpha protein untreated and treated with FeCl2
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-HIF-1-alpha Antibody, hydroxyproline (Pro564) is a rabbit polyclonal antibody for detection of HIF-1-alpha also known as Hypoxia-inducible factor 1-alpha, HIF1-alpha & has been validated in WB, PIA. 主要应用 - Western Blotting
应用说明 Peptide Inhibition Analysis: A representative lot of this antibody was used for peptide blocking using an in vitro transcribed and translated HIF-1-alpha protein. 生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to the C-terminal VHL recognition site of human HIF-1-alpha, hydroxyproline (Pro564). 表位 C-terminal VHL recognition site 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes the C-terminal VHL recognition site of HIF-1-alpha hydroxylated at Pro564. 品种反应性 Human Species Reactivity Note Demonstrated to react with Human. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_001521
Entrez基因汇总 This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. 基因符号 - PASD8
- HIF1A
- BHLHE78
- MOP1
修改 - Hydroxylation
纯化方法 Affinity purified UniProt编号 - Q16665
UniProt汇总 FUNCTION: Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions activates the transcription of over 40 genes, including, erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP.
SUBUNIT STRUCTURE: Interacts with the HIF1A beta/ARNT subunit; heterodimerization is required for DNA binding. Interacts with COPS5; the interaction increases the transcriptional activity of HIF1A through increased stability. Interacts with EP300 (via TAZ-type 1 domains); the interaction is stimulated in response to hypoxia and inhibited by CITED2. Interacts with CREBBP (via TAZ-type 1 domains). Interacts with NCOA1, NCOA2, APEX and HSP90. Interacts (hydroxylated within the ODD domain) with VHLL (via beta domain); the interaction, leads to polyubiquitination and subsequent HIF1A proteasomal degradation. During hypoxia, sumoylated HIF1A also binds VHL; the interaction promotes the ubiquitination of HIF1A. Interacts with SENP1; the interaction desumoylates HIF1A resulting in stabilization and activation of transcription. Interacts (Via the ODD domain) with ARD1A; the interaction appears not to acetylate HIF1A nor have any affect on protein stability, during hypoxia. Interacts with RWDD3; the interaction enhances HIF1A sumoylation. Interacts with TSGA10 By similarity. Interacts with RORA (via the DNA binding domain); the interaction enhances HIF1A transcription under hypoxia through increasing protein stability. Interaction with PSMA7 inhibits the transactivation activity of HIF1A under both normoxic and hypoxia-mimicking conditions. Interacts with USP20. Interacts with GNB2L1/RACK1; promotes HIF1A ubiquitination and proteasome-mediated degradation.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note: Cytoplasmic in normoxia, nuclear translocation in response to hypoxia. Colocalizes with SUMO1 in the nucleus, under hypoxia.
TISSUE SPECIFICITY: Expressed in most tissues with highest levels in kidney and heart. Overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors.产品使用声明 质量保证 Evaluated by Western Blot using in vitro transcribed and translated HIF-1-alpha protein untreated and treated with FeCl2.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected in vitro transcribed and translated HIF-1-alpha protein treated with FeCl2.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µL
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