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merck millipore,默克密理博,07-1533,Anti-JMJD3 (NT) Antibody
产品名称：Anti-JMJD3 (NT) Antibody
产品型号：07-1533
Use Anti-JMJD3 (N-terminus) Antibody (Rabbit Polyclonal Antibody) validated in IHC, ICC, ELISA, WB to detect JMJD3 (N-terminus) also known as JmjC domain-containing protein 3.
merck millipore,默克密理博,07-1533,Anti-JMJD3 (NT) Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M, H WB, IHC, ICC, ELISA Rb Purified Polyclonal Antibody 描述 产品目录编号 07-1533 描述 Anti-JMJD3 (NT) Antibody Alternate Names - jumonji domain containing 3, histone lysine demethylase
- JMJD
- JmjC domain-containing protein 3
- Jumonji domain-containing protein 3
- Lysine demethylase 6B
- jumonji domain containing 3
- jumonji domain containing 3
背景信息 JMJD3 (Jumonji domain-containing protein 3) is part of the family of novel JmjC domain-containing histone demethylation (JHDM) enzymes. Histone demethylation by JHDM proteins requires cofactors Fe(II) and alpha-ketoglutarate. JMJD3 specifically demethylates Lys27 on histone H3, demethylating both the trimethylated and dimethylated form. JMJD3 plays a role in the regulation of posterior development by regulating HOX gene expression. It is also involved in the inflammatory response by regulating gene expression and macrophage differentiation 产品信息 格式 Purified 控制 - HeLa cells
演示 Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. 应用 应用 Use Anti-JMJD3 (N-terminus) Antibody (Rabbit Polyclonal Antibody) validated in IHC, ICC, ELISA, WB to detect JMJD3 (N-terminus) also known as JmjC domain-containing protein 3. 主要应用 - Western Blotting
- Immunohistochemistry
- Immunocytochemistry
- ELISA
应用说明 ELISA:
This antibody is reported by an
outside laboratory to be suitable for ELISA.
Immunohistochemistry:
This antibody is reported by an outside laboratory to be suitable for immuno-histochemistry.
Immunocytochemistry Analysis:
Confocal immunofluorescence analysis of HeLa and NIH/3T3 using 1:100 dilution of anti-JMJD3 (N-Terminus) (Red). Actin filaments have been labeled with Alexa Fluor 488 -Phalloidin (Green).
Western Blot Analysis:
The antibody recognizes JMJD3 in HeLa cell lysates.
生物信息 免疫原品种 This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human JMJD3. 表位 N-terminus 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Recognizes the N-terminal region of human JMJD3. Additional lower MW bands are detected in some lysates that may be degradation products. 品种反应性 MouseHuman Species Reactivity Note Human and mouse. Not tested in other species. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_001073893
基因符号 - KIAA0346
- EC 1.14.11
- KDM6B
纯化方法 Protein G chromatography UniProt编号 - O15054
UniProt汇总 FUNCTION: Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code. Demethylates trimethylated and dimethylated H3 'Lys-27'. Plays a central role in regulation of posterior development, by regulating HOX gene expression. Involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation. Ref.5 Ref.6
Cofactor Ascorbate By similarity.
Fe(2+). Ref.5
SUBUNIT STRUCTURE: Interacts with TLE1 By similarity. Component of the MLL4 complex, at least composed of MLL4, ASH2L, RBBP5, WDR5, and JMJD3.
SUBCELLULAR lOCATION: NucleusProbable.
INDUCTION: By 12-O-tetradecanoylphorbol-13-acetate (TPA) in myeloid leukemia cells. Ref.4
SIMILARITY: Belongs to the UTX family.
Contains 1 JmjC domain.
产品使用声明 质量保证 Immunocytochemisty:
Recognizes JMJD3 in HeLa, NIH 3T3, and A431 cells by cytochemistry (1:50 – 1:100 dilution).
使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial to pellet the solution. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
包装信息 数量 400 µL
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