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Replacement Information Replacement Information 07-1496 is a recommended replacement for AB3111. 重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Rb, H, R, M, B, Ht, Gp, Ch WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 07-1496 Replaces AB3111 描述 Anti-CaM Kinase II Antibody 背景信息 The Ca2+/Calmodulin-Dependent Kinase family includes CaM Kinases I through IV, Phosphorylase Kinase, and MLCK. The CaM Kinases are inactive in the absence of stimuli; binding to Ca2+/Calmodulin induces conformational changes and activation. CaM Kinases have broad substrate specificity, with many cellular functions. 产品信息 格式 Affinity Purified 控制 - HEK293 cell lysate
演示 Purified rabbit polyclonal IgG in buffer containing borate buffered saline and phosphate buffered saline containing 0.05% sodium azide. 应用 应用 Anti-CaM Kinase II Antibody is an antibody against CaM Kinase II for use in WB. 主要应用 - Western Blotting
应用说明 Optimal working dilutions must be determined by end user.
生物信息 免疫原品种 Synthetic peptide corresponding to the N-terminus of Rat CaMK II 表位 N-terminus 宿主 Rabbit 特异性 Recognizes proteins of 50-72 kDa, corresponding to apparent molecular mass of CaMKII isoforms on SDS-PAGE immunoblots. Recombinant rat calmodulin-dependent protein kinase II α subunits expressed in sf9 insect cells are also detected. As the sequences of the rat α, δ, and γ isoforms are conserved over this amino terminus region, this antibody is expected to recognize the δ, γ, and α isoforms. Antibody is not expected to cross-react with the β isoform. Proteins of unknown identity may be detected on immunoblot analysis with some lysates (40, 45 and 90 kDa). 同种型 IgG 品种反应性 RabbitHumanRatMouseBovineHamsterGuinea PigChicken Species Reactivity Note Human, rat, mouse, bovine, hamster, guinea pig, chicken, and rabbit. 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_015981.2
Entrez基因汇总 The product of this gene belongs to the Serine/Threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This enzyme is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Two transcript variants encoding distinct isoforms have been identified for this gene 基因符号 - EC 2.7.11.17
- CAMK2A
- KIAA0968
- CAMKA
纯化方法 Affinity Purfied UniProt编号 - Q9UQM7
UniProt汇总 FUNCTION: SwissProt: Q9UQM7 # CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity (By similarity).
SIZE: 478 amino acids; 54030 Da
SUBUNIT: CAMK2 is composed of four different chains: alpha, beta, gamma, and delta. The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. Interacts with BAALC, MPDZ, SYN1 and SYNGAP1 (By similarity).
SUBCELLULAR LOCATION: Cell junction, synapse, presynaptic cell membrane (By similarity). Cell junction, synapse (By similarity). Note=Postsynaptic lipid rafts (By similarity).
SIMILARITY: SwissProt: Q9UQM7 ## Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. & Contains 1 protein kinase domain.产品使用声明 质量保证 Routinely evaluated by Western Blot on HEK293 lysates.
Western Blot Analysis:
1:500 to 1:1,000 dilution of this lot detected CaM Kinase II on 10 μg of HEK293 lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C in undiluted aliquots from date of receipt. For optimal storage, aliquot and store at -20°C or -70°C.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 100 µL
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