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Replacement Information Replacement Information 07-1436 is a recommended replacement for AB1486P. 重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M, H WB, ELISA Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 07-1436 Replaces AB1486P 描述 Anti-PDGF-AA Antibody Alternate Names - Platelet-derived growth factor alpha polypeptide
- Platelet-derived growth factor alpha
- Platelet-derived growth factor alpha chain
背景信息 Platelet-Derived Growth Factor (PDGF) is a dimer of A (17 kDa) and B (14 kDa) chains (AA, AB, or BB). Its receptors are transmembrane receptor tyrosine kinases, PDGF Receptors type A and B. The type A PDGF receptor binds all three PDGFs with high affinity, whereas the type B PDGF receptor is selective for PDGF-BB. PDGF signals as a growth and survival factor, as it is a potent activator of the PI3 Kinase pathway. PDGF signaling appears to play an essential role in the development of tumors of glial origin. 产品信息 格式 Affinity Purified 控制 - C2C12 cell lysate
演示 Purified rabbit polyclonal IgG lyophilized from PBS, pH 7.2. 应用 应用 Detect PDGF-AA using this Anti-PDGF-AA Antibody validated for use in IH(P), ELISA, WB, NEUT. 主要应用 - Western Blotting
- ELISA
应用说明 Immunohistochemisty (paraffin): PDGF representative staining pattern/morphology in prostate cancer. Tissue was pretreated with citrate pH 6, antigen retrieval. A previous lot of this antibody was diluted to 1:100, using IHC-Select® Detection with HRP-DAB.
ELISA: 0.5 μg/mL dilution of a previous lot allowed detection (in direct ELISA) of 0.2-0.4 ng/well when human PDGF-AA is used to coat the plate.
Neutralization: To yield ½ maiximal inhibition (ND50) of the biological activity of hPDGF-AA (5.0 ng/mL), a concentration of 0.05-0.07 μg/mL of a previous lot of this antibody was required.
Optimal working dilutions must be determined by the end user.
生物信息 免疫原品种 Highly pure (>98%) recombinant human PDGF-AA. 宿主 Rabbit 特异性 Reacts with PDGF-AA 同种型 IgG 品种反应性 MouseHuman Species Reactivity Note Human, Mouse 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_002607.4
Entrez基因汇总 The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer or as a heterodimer with the platelet-derived growth factor beta polypeptide, where the dimers are connected by disulfide bonds. Studies using knockout mice have shown cellular defects in oligodendrocytes, alveolar smooth muscle cells, and Leydig cells in the testis; nockout mice die either as embryos or shortly after birth. Two splice variants have been identified for this gene. [provided by RefSeq]. 基因符号 - PDGF1
- PDGFA
- PDGF-1
- PDGF-A
纯化方法 Affinity Purfied UniProt编号 - P04085
UniProt汇总 FUNCTION: Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. Binding of this growth factor to its affinity receptor elicits a variety of cellular responses. It is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heals the wound.
SUBUNIT: Antiparallel disulfide-linked dimer of non-identical (A and B) chains. Homodimers of A and B chains are implicated in transformation processes. Interacts with CSPG4.
SUBCELLULAR LOCATION: Secreted.
ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by alternative splicing.
Name Long
Isoform ID P04085-1
This is the isoform sequence displayed in this entry.
Name Short
Isoform ID P04085-2
Features which should be applied to build the isoform sequence: VSP_004602, VSP_004603.
DOMAIN: The long form contains a basic insert which acts as a cell retention signal.
MISCELLANEOUS: A-A and B-B, as well as A-B, dimers can bind to the PDGF receptor.
SIMILARITY: Belongs to the PDGF/VEGF growth factor family.
产品使用声明 质量保证 Routinely evaluated by Western Blot on C2C12 lysates.
Western Blot Analysis:
1:500 dilution of this lot detected PDGF-AA on 10 μg of C2C12 lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 The lyophilized antibody is stable at room temperature for at least one month and for up to one year when kept at -20ºC. When reconstituted in sterile water to a concentration of 0.1-1.0 mg/mL, the antibody is stable for at least six weeks at 2-8ºC.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 100 µL
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