merck millipore,默克密理博,70839,Origami™ B(DE3)pLysS Competent Cells

搜索"Merck Millipore 密理愽"产品

Merck Millipore 默克密理愽

merck millipore,默克密理博,70839,Origami™ B(DE3)pLysS Competent Cells - Novagen

描述
概述	Origami™ B host strains carry the same mutations in trxB and gor as the original Origami strains, except that they are derived from a lacZY mutant of BL21 to enable precise control of expression levels by adjusting the concentration of IPTG. Thus the Origami B strains combine the desirable characteristics of BL21, Tuner™, and Origami strains in one strain background. The mutations in trxB and gor are selectable on kanamycin and tetracycline, respectively; therefore, these strains cannot be used with plasmids that can only selected with kanamycin or tetracycline. These strains also include the lon and ompT deficiences of BL21, which increase protein stability. DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG. pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.
概述	Genotype: F^- ompT hsdS_B(r_B^- m_B^-) gal dcm lacY1 ahpC (DE3) gor522:: Tn10 trxB pLysS (Cam^R, Kan^R, Tet^R)
产品目录编号	70839
品牌系列	Novagen®

产品信息
部件
保证高效	Guaranteed efficiency: > 2 x 10⁶ cfu/μg

储存和货运信息
货运代码	Dry Ice Only
毒性	Multiple Toxicity Values, refer to MSDS
储存	≤ -70°C
无需冷冻	No
布鲁克黑文许可证	This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.

merck millipore,默克密理博,70839,Origami™ B(DE3)pLysS Competent Cells - Novagen

whatman,沃特曼,nalgene,耐洁,merck millipore,默克密理愽,sartorius,赛多利斯,pall,颇尔
thermo fisher,赛默飞世尔,GE,MN,macherey-nagel,advantec,wheaton,brand,普兰德,welch,airtech