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品种反应性 主要应用 宿主 格式 抗体类型 Xn, M, H, B, Av, Ch WB Rb Purified Polyclonal Antibody 描述 产品目录编号 07-108 品牌系列 Upstate
商名 - Upstate
描述 Anti-Histone H4 Antibody Alternate Names - histone cluster 1, H4a
- H4 histone family, member A
- histone 1, H4a
- histone 1
- H4a
背景信息 Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the 'beads on a string' structure. 产品信息 格式 Purified 控制 - HeLa cell extract.
演示 Purified rabbit polyclonal IgG in buffer containing 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% Sodium Azide. 应用 应用 Use Anti-Histone H4 Antibody (Rabbit Polyclonal Antibody) validated in WB to detect Histone H4 also known as Histone H4. 主要应用 - Western Blotting
生物信息 免疫原品种 Calf thymus histone H4. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Recognizes Histone H4, Mr 10 kDa. 同种型 IgG 品种反应性 XenopusMouseHumanBovineAvianChicken Species Reactivity Note Human, mouse, bovine, chicken and Xenopus. Broad species cross-reactivity expected. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_778224
Entrez基因汇总 Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. 基因符号 - H4/E
- HIST1H4A
- H4/J
- HIST1H4F
- HIST1H4L
- H4FN
- H4FA
- H4FH
- HIST1H4H
- H4F2
- H4FM
- H4/A
- H4FK
- H4FG
- HIST2H4
- H4FB
- H4/K
- HIST1H4I
- H4/N
- HIST1H4B
- H4FE
- H4/M
- H4/a
- HIST1H4E
- HIST4H4
- HIST2H4A
- H4FC
- H4FI
- H4/H
- HIST1H4C
- H4/G
- HIST1H4K
- H4FJ
- H4FD
- H4/I
- H4/B
- H4/D
- H4/C
- HIST1H4J
- HIST1H4D
纯化方法 Protein A chromatography UniProt编号 - P62805
UniProt汇总 FUNCTION: SwissProt: P62805 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
SIZE: 103 amino acids; 11367 Da
SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
SUBCELLULAR LOCATION: Nucleus.
PTM: Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage (By similarity). & Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
SIMILARITY: SwissProt: P62805 ## Belongs to the histone H4 family.产品使用声明 质量保证 Routinely evaluated by western blot on acid extracts of HeLa cells treated with sodium butyrate.
Western Blot Analysis:
0.1-0.5 μg/mL of this lot detected Histone H4 in acid extracts of HeLa cells treated with or without 5 mM sodium butyrate. This lot did not cross react with Histone H2A, Histone H2B or Histone H3.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt. 包装信息 数量 200 µL
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