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merck millipore,默克密理博,06-942,Anti-acetyl-Histone H3 (Lys9) Antibody
产品名称:Anti-acetyl-Histone H3 (Lys9) Antibody
产品型号:06-942
Anti-acetyl-Histone H3 (Lys9) Antibody is a Rabbit Polyclonal Antibody for detection of Histone H3 acetylated on lysine 9. This purified Ab, also known as Anti-H3K9Ac, is specificity verified by dot b More>>
merck millipore,默克密理博,06-942,Anti-acetyl-Histone H3 (Lys9) Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H, M FC, WB, ChIP Rb Purified Polyclonal Antibody 描述 产品目录编号 06-942 Replaces 04-1003 品牌系列 Upstate
商名 - Upstate
描述 Anti-acetyl-Histone H3 (Lys9) Antibody Alternate Names - histone cluster 3, H3
- H3K9Ac
- Histone H3 (acetyl K9)
- H3 histone family, member T
- histone 3, H3
- histone 3
- H3
背景信息 Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. 产品信息 格式 Purified 演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide with 30% glycerol. 应用 应用 Anti-acetyl-Histone H3 (Lys9) Antibody is a Rabbit Polyclonal Antibody for detection of Histone H3 acetylated on lysine 9. This purified Ab, also known as Anti-H3K9Ac, is specificity verified by dot blot (DB), published in peer reviewed journals, and validated in WB, ChIP, ChIP-seq, FC. 主要应用 - Flow Cytometry
- Western Blotting
- Chromatin Immunoprecipitation (ChIP)
应用说明 Chromatin Immunoprecipitation/ChIP-seq Analysis: A representative lot immunoprecipitated acetyl-Histone H3 (Lys9) in 5 µg of HeLa cell lysate.
Dot Blot Analysis: A 1:1,000 dilution from a representative lot detected acetyl-Histone H3 (Lys9) in an Absurance™ Histone H3 Antibody Specificity Array (Cat. No. 16-667) and an Absurance™ Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).
Flow Cytometry Analysis: 0.25 µg of this antibody from a representative lot detected acetyl-Histone H3 (Lys9) in 1X10E6 HEK293 cells.生物信息 免疫原品种 KLH-conjugated synthetic peptide (ARTKQTARK(Ac)STG-C) corresponding to amino acids 1-12 of Histone H3 with acetylated Lys9. 表位 Surrounding H3K9ac 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Histone H3 acetylated at position 9. 同种型 IgG 品种反应性 RatHumanMouse Species Reactivity Note Human, Mouse, Rat, although this peptide sequence is identical in a wide range of animal and plant species. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_003520
基因符号 - MGC126886
- HIST1H3A
- H3FA
- HIST1H3B
- H3FL
- HIST1H3C
- H3FC
- HIST1H3D
- H3FB
- HIST1H3E
- H3FD
- HIST1H3F
- H3FI
- HIST1H3G
- H3FH
- HIST1H3H
- H3FK
- HIST1H3I
- H3FF
- HIST1H3J
- H3FJ
- HIST3H3
- H3FT
- H3T
修改 - Acetylation
纯化方法 Protein A purfied UniProt编号 - Q16695
产品使用声明 质量保证 Evaluated by Western Blotting in sodium butyrate treated HeLa cell lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected acetyl-Histone H3 (Lys9) in 10 µg of sodium butyrate treated HeLa cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.包装信息 数量 200 µg
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