merck millipore,默克密理博,06-866,Anti-acetyl-Histone H4 Antibody

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merck millipore,默克密理博,06-866,Anti-acetyl-Histone H4 Antibody
产品名称：Anti-acetyl-Histone H4 Antibody
产品型号：06-866
Use Anti-acetyl-Histone H4 Antibody (Rabbit Polyclonal Antibody) validated in ChIP, WB, ICC to detect acetyl-Histone H4 also known as H4 histone family member A, histone 1-H4a.

merck millipore,默克密理博,06-866,Anti-acetyl-Histone H4 Antibody

产品介绍

merck millipore,默克密理博,06-866,Anti-acetyl-Histone H4 Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
Eu, H, T	ChIP, WB, ICC	Rb	Serum	Polyclonal Antibody

描述
产品目录编号	06-866
品牌系列	Upstate
商名	Upstate
描述	Anti-acetyl-Histone H4 Antibody
Alternate Names	histone cluster 1, H4a H4 histone family, member A histone 1, H4a histone 1 H4a
背景信息	Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).

产品信息
格式	Serum
控制	TSA-treated Jurkat cells. For a negative control, perform a no-antibody immunoprecipitation by incubating the supernatant fraction with 60μl of Salmon Sperm DNA/Protein A Agarose- 50% Slurry. Transcriptionally unactivated DNA samples should be prepared as controls for PCR.
演示	Whole antiserum containing 0.05% sodium azide.

应用
应用	Use Anti-acetyl-Histone H4 Antibody (Rabbit Polyclonal Antibody) validated in ChIP, WB, ICC to detect acetyl-Histone H4 also known as H4 histone family member A, histone 1-H4a.
主要应用	Chromatin Immunoprecipitation (ChIP) Western Blotting Immunocytochemistry
应用说明	Chromatin Immunoprecipitation: 5-10 μL of this lot immunoprecipitated transcriptionally active chromatin containing acetylated histone H4 from 2 X 106 serum stimulated HeLa cells.

生物信息
免疫原品种	KLH-conjugated peptide corresponding to a.a. 2-19 of Tetrahymena histone H4 [AGGAcKGGAcKGMGAcKVGAAcKRHS-C], acetylated on lysines 5, 8, 12 and 16.
表位	a.a. 2-19
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
特异性	Acetylated histone H4, acetylated histone H2B from Tetrahymena and weakly cross-reacts with acetylated histone H2B from HeLa cells, may cross-react with other acetylated proteins.
同种型	IgG
品种反应性	EukaryoteHumanTetrahymena
Species Reactivity Note	Human and Tetrahymena. Other species not tested, but expected to cross-react since Histone H4 is well conserved.
抗体类型	Polyclonal Antibody
Entrez基因编号	NM_175054
Entrez基因汇总	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
基因符号	H4FE H4/M H4/a IST1H4E HIST4H4 HIST2H4A H4FC H4FI H4/H HIST1H4C H4/G HIST1H4K 4FJ H4FD H4/I H4/B H4/D H4/C HIST1H4J HIST1H4D H4/E HIST1H4A H4/J HIST1H4F HIST1H4L H4FN H4FA H4FH HIST1H4H H4F2 H4FM H4/A H4FK H4FG HIST2H4 H4FB H4/K HIST1H4I H4/N HIST1H4B
修改	Acetylation
纯化方法	Unpurified
UniProt编号	P62805
UniProt汇总	FUNCTION: SwissProt: P62805 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. SIZE: 103 amino acids; 11367 Da SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. SUBCELLULAR LOCATION: Nucleus. PTM: Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage (By similarity). & Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. SIMILARITY: SwissProt: P62805 ## Belongs to the histone H4 family.

产品使用声明
质量保证	Routinely evaluated by western blot on Tetrahymena macronuclei or acid extracted proteins from HeLa cells treated with 5 mM sodium butyrate. Western Blot Analysis: 1:2000 dilution of this lot detected acetylated histone H4 in acid extracted proteins from HeLa cells treated with 5 mM sodium butyrate. Sodium butyrate, an inhibitor of deacetylases, was used to enhance detection of acetylated histone H4.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品使用声明

质量保证

Routinely evaluated by western blot on Tetrahymena macronuclei or acid extracted proteins from HeLa cells treated with 5 mM sodium butyrate.

Western Blot Analysis:
1:2000 dilution of this lot detected acetylated histone H4 in acid extracted proteins from HeLa cells treated with 5 mM sodium butyrate. Sodium butyrate, an inhibitor of deacetylases, was used to enhance detection of acetylated histone H4.

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.