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merck millipore,默克密理博,S7815,CpG WIZ ERα - Methylation specific PCR assay
产品名称：CpG WIZ ERα - Methylation specific PCR assay
产品型号：S7815
The components of the CpG WIZ ERalpha Amplification Kit include those required for PCR amplification after bisulfite modification of DNA samples. Sufficient reagents are provided to analyze 25 samples More>>

merck millipore,默克密理博,S7815,CpG WIZ ERα - Methylation specific PCR assay

产品介绍

merck millipore,默克密理博,S7815,CpG WIZ ERα - Methylation specific PCR assay

描述
产品目录编号	S7815
品牌系列	Chemicon®
商名	Chemicon CpG Wiz
描述	CpG WIZ® ERα - Methylation specific PCR assay
概述	Methylation of cytosines located 5' to guanosine is known to have a profound effect on the expression of several eukaryotic genes (Bird, 1992 ). In normal cells, methylation occurs predominantly in CG-poor regions, while CG-rich areas, called CpG islands, remain unmethylated. Aberrant methylation of normally unmethylated CpG islands has been documented as a relatively frequent event in immortalized and transformed cells (Antequera, 1990) and has been associated with transcriptional inactivation of defined tumor suppressor genes in human cancers (Merlo, 1995). The Estrogen Receptor Alpha (ERalpha) gene is expressed at reduced levels in certain tumor types as a result of hypermethylation of its promoter region (Kim SJ, 2004; Woo IS, 2004). Methylation-specific PCR (MSP) is a new technology for sensitive detection of abnormal gene methylation utilizing small amounts of DNA (Herman, 1996 ). This process employs an initial bisulfite reaction to modify the DNA, followed by PCR amplification with specific primers designed to distinguish methylated from unmethylated DNA.

产品信息
部件	Universal 10X PCR Buffer / 265 μL (blue cap) / 90396 / -15°C to -25°C The components of the CpG WIZ® ERalpha Amplification Kit include those required for PCR amplification after bisulfite modification of DNA samples. Sufficient reagents are provided to analyze 25 samples with appropriate controls. U Primer Set7.5 μM each primer (25X) / 35 μL (clear cap) / 90633 / -15°C to -25°C M Primer Set7.5 μM each primer (25X) / 35 μL (red cap) / 90634 / -15°C to -25°C W Primer Set7.5 μM each primer (25X) / 35 μL (green cap) / 90635 / -15°C to -25°C U control DNA0.1 μg/μL / 50 μL (clear cap) / 90393 / -15°C to -25°C M control DNA0.1 μg/μL / 50 μL (red cap) / 90394 / -15°C to -25°C W control DNA0.05 μg/μL / 50 μL (green cap) / 90395 / -15°C to -25°C

产品信息

部件

Universal 10X PCR Buffer / 265 μL (blue cap) / 90396 / -15°C to -25°C
The components of the CpG WIZ® ERalpha Amplification Kit include those required for PCR amplification after bisulfite modification of DNA samples. Sufficient reagents are provided to analyze 25 samples with appropriate controls.
U Primer Set7.5 μM each primer (25X) / 35 μL (clear cap) / 90633 / -15°C to -25°C
M Primer Set7.5 μM each primer (25X) / 35 μL (red cap) / 90634 / -15°C to -25°C
W Primer Set7.5 μM each primer (25X) / 35 μL (green cap) / 90635 / -15°C to -25°C
U control DNA0.1 μg/μL / 50 μL (clear cap) / 90393 / -15°C to -25°C
M control DNA0.1 μg/μL / 50 μL (red cap) / 90394 / -15°C to -25°C
W control DNA0.05 μg/μL / 50 μL (green cap) / 90395 / -15°C to -25°C

应用
应用	The components of the CpG WIZ ERalpha Amplification Kit include those required for PCR amplification after bisulfite modification of DNA samples. Sufficient reagents are provided to analyze 25 samples.
主要应用	PCR
应用说明	Principles of the Technique Use of either the CpGenome™ DNA Modification Kit (Cat. No. S7820) or the CpGENOME™ Fast DNA Modification Kit (Cat. No. S7824) facilitates the initial bisulfite reactions, while the CpG WIZ® ERalpha Amplification Kit contains the reagents required for the gene-specific PCR amplification reactions.

生物信息
品种反应性	Human
Entrez基因编号	NM_000125.2
Entrez基因汇总	This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5' UTRs and use different promoters.
基因符号	ER-alpha ESR1 ESR Era ER NR3A1 ESRA DKFZp686N23123
UniProt编号	P03372
UniProt汇总	FUNCTION: SwissProt: P03372 # Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. SIZE: 595 amino acids; 66216 Da SUBUNIT: Binds DNA as a homodimer. Can form a heterodimer with ESR2. Interacts with NCOA3, NCOA5 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Interacts with NCOA7 in a ligand-inducible manner. Interacts with PHB2, PELP1 and UBE1C. Interacts with AKAP13. Interacts with CUEDC2. Interacts with JARID1A. Interacts with SMARD1. Interacts with SLC30A9 (By similarity). Interacts with HEXIM1 and MAP1S. Interacts with PBXIP1. SUBCELLULAR LOCATION: Nucleus. DOMAIN: SwissProt: P03372 Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal steroid-binding domain. PTM: Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity. SIMILARITY: Belongs to the nuclear hormone receptor family. NR3 subfamily. & Contains 1 nuclear receptor DNA-binding domain. MISCELLANEOUS: In the absence of ligand, steroid hormone receptors are thought to be weakly associated with nuclear components; hormone binding greatly increases receptor affinity. The hormone- receptor complex appears to recognize discrete DNA sequences upstream of transcriptional start sites.

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.