merck millipore,默克密理博,05-465,Anti-GRK 2/3 (βARK 1/2) Antibody, clone C5/1.1

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merck millipore,默克密理博,05-465,Anti-GRK 2/3 (βARK 1/2) Antibody, clone C5/1.1
产品名称：Anti-GRK 2/3 (βARK 1/2) Antibody, clone C5/1.1
产品型号：05-465
Detect GRK 2/3 (βARK 1/2) using this Anti-GRK 2/3 (βARK 1/2) Antibody, clone C5/1.1 validated for use in IP & WB.

merck millipore,默克密理博,05-465,Anti-GRK 2/3 (βARK 1/2) Antibody, clone C5/1.1

产品介绍

merck millipore,默克密理博,05-465,Anti-GRK 2/3 (βARK 1/2) Antibody, clone C5/1.1

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
Rb, H, R, M, B, Ch, Po	WB	M	Purified	Monoclonal Antibody

描述
产品目录编号	05-465
品牌系列	Upstate
商名	Upstate
描述	Anti-GRK 2/3 (βARK 1/2) Antibody, clone C5/1.1
Alternate Names	beta adrenergic receptor kinase 1 G-protein coupled receptor kinase 2 adrenergic, beta, receptor kinase 1 beta receptor kinase 1
背景信息	The beta-adrenergic receptor kinase specifically phosphorylates the agonist-occupied form of the beta-adrenergic and related G protein-coupled receptors. Overall, the ADRBK2 enzyme, also known as GRK3, has 85% amino acid similarity with ADRBK1, with the protein kinase catalytic domain having 95% similarity. The ADRBK2 mRNA is approximately 8 kilobases with a distribution similar to that of ADRBK1. These data suggest the existence of a family of receptor kinases which may serve broadly to regulate receptor function. GRK2 phosphorylates the beta2 adrenergic receptor. It is a ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors.

产品信息
格式	Purified
控制	Human breast carcinoma tissue, mouse heart tissue, SK OV 3 cell lysates and NIH 3T3 cell lysates. Included Positive Antigen Control: Catalog # 12-305, 3T3/A31 cell lysate. Add 2.5 µL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced lysate per lane for minigels.
演示	Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl containing 0.05% sodium azide. Frozen solution.

应用
应用	Detect GRK 2/3 (βARK 1/2) using this Anti-GRK 2/3 (βARK 1/2) Antibody, clone C5/1.1 validated for use in IP & WB.
主要应用	Western Blotting
应用说明	Immunoprecipitation: A previous lot of this antibody was used to immunoprecipitate GRK2/3 from transfected cells, as shown by an independent laboratory.

生物信息
免疫原品种	Partial GST fusion protein corresponding to the GRK3 (βARK2) carboxyl terminus (a.a. 467-688). This immunogen shares homology with GRK2 (βARK1). The minimal epitope recognized by the antibody is a.a. 483-485. Clone C5/1.1.
表位	a.a. 483-485
克隆	C5/1.1
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Mouse
特异性	This antibody recognizes GRK2/3, Mr 80 kDa. Additional bands at 85 and 50 kDa are also detected when using higher concentrations of the antibody.
同种型	IgG2aκ
品种反应性	RabbitHumanRatMouseBovineChickenPig
Species Reactivity Note	Rat, human, mouse, chicken, bovine, porcine, and rabbit.
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_001619, NM_005160
Entrez基因汇总	The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart.
基因符号	BARK1 ADRBK1 Beta-ARK-1 GRK2 BARK FLJ16718 BETA-ARK1
纯化方法	Protein G purfied
UniProt编号	P25098
UniProt汇总	FUNCTION: SwissProt: P25098 # Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. SIZE: 689 amino acids; 79574 Da SUBUNIT: Interacts with GIT1 (By similarity). SIMILARITY: SwissProt: P25098 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 PH domain. & Contains 1 protein kinase domain. & Contains 1 RGS domain. ... hide » see more »

产品使用声明
质量保证	Routinely evaluated by immunoblot on RIPA lysates from mouse 3T3/A31 fibroblasts, bovine brain cytosol preparation, rat L6 myoblasts, human A431 carcinoma or human HeLa cell lysates. Western Blot Analysis: 0.1-1 µg/mL of this lot detected GRK2/3 (βARK 1/2) in RIPA lysates from mouse 3T3/A31 fibroblasts. Previous lots of this antibody detected GRK2/3 in bovine brain cytosol preparation, rat L6 myoblasts, human A431 carcinoma and human HeLa cell RIPA lysates.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品使用声明

质量保证

Routinely evaluated by immunoblot on RIPA lysates from mouse 3T3/A31 fibroblasts, bovine brain cytosol preparation, rat L6 myoblasts, human A431 carcinoma or human HeLa cell lysates.

Western Blot Analysis:
0.1-1 µg/mL of this lot detected GRK2/3 (βARK 1/2) in RIPA lysates from mouse 3T3/A31 fibroblasts. Previous lots of this antibody detected GRK2/3 in bovine brain cytosol preparation, rat L6 myoblasts, human A431 carcinoma and human HeLa cell RIPA lysates.

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.