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品种反应性 主要应用 宿主 格式 抗体类型 Xn, B, H, M, Po, R WB, ICC, IHC Rb Purified Polyclonal Antibody 描述 产品目录编号 06-661 Replaces MABT145 品牌系列 Upstate
商名 - Upstate
描述 Anti-Calreticulin Antibody 产品信息 格式 Purified 演示 0.1M Tris-glycine, pH 7.0, 0.15M NaCl and 0.05% sodium azide. 应用 应用 Anti-Calreticulin Antibody is an antibody against Calreticulin for use in IC, IH & WB. 主要应用 - Western Blotting
- Immunocytochemistry
- Immunohistochemistry
生物信息 免疫原品种 peptide (QAKDEL) corresponding to the C-terminus (amino acids 412-417) of human calreticulin 宿主 Rabbit 特异性 calreticulin; does not cross react with other endoplasmic reticulum proteins 同种型 IgG 品种反应性 XenopusBovineHumanMousePigRat 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_004343
Entrez基因汇总 Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, calreticulin can act as an important modulator of the regulation of gene transcription by nuclear hormone receptors. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin but calreticulin is not a Ro/SS-A antigen. Earlier papers referred to calreticulin as an Ro/SS-A antigen but this was later disproven. Increased autoantibody titer against human calreticulin is found in infants with complete congenital heart block of both the IgG and IgM classes. 基因符号 - SSA
- CALR
- calreticulin
- cC1qR
- CRTC
- HACBP
- CRP55
- grp60
- FLJ26680
- Calregulin
- ERp60
- RO
纯化方法 Protein A chromatography UniProt编号 - P27797
UniProt汇总 FUNCTION: SwissProt: P27797 # Molecular calcium binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export.
SIZE: 417 amino acids; 48142 Da
SUBUNIT: Monomer. Component of an EIF2 complex at least composed of CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Interacts with PDIA3/ERp57 and with NR3C1 (By similarity).
SUBCELLULAR LOCATION: Endoplasmic reticulum lumen. Cytoplasm, cytosol. Secreted, extracellular space, extracellular matrix. Cell surface. Note=Also found in cell surface (T cells), cytosol and extracellular matrix. Associated with the lytic granules in the cytolytic T-lymphocytes.
DOMAIN: SwissProt: P27797 Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity. & The interaction with glycans occurs through a binding site in the globular lectin domain. & The zinc binding sites are localized to the N-domain. & Associates with PDIA3 through the tip of the extended arm formed by the P-domain.
SIMILARITY: Belongs to the calreticulin family.产品使用声明 质量保证 routinely evaluated by immunoblot on RIPA lysates from murine 3T3/NIH fibroblasts 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 200 µg
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