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品种反应性 主要应用 宿主 格式 抗体类型 M, H WB Rb Purified Polyclonal Antibody 描述 产品目录编号 06-494 品牌系列 Upstate
商名 - Upstate
描述 Anti-IκB-α Antibody 背景信息 The NF-kappaB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IkappaB-alpha protein. In response to many different NF-kappaB-inducing agentsincluding T-cell mitogens, proinflammatory cytokines, and viral transactivatorsthe inhibitory IkappaB-alpha is rapidly phosphorylated and degraded. Phosphorylation of IkappaB-alpha at Ser32 and Ser36 has been shown to stimulate conjugation with ubiquitin and subsequent degradation of IkappaB-alpha. Phosphorylation of IkappaB-alpha at Ser32 and Ser36 is critical for activation of NF-kappaB, which then translocates to the nucleus and induces transcription of genes that protect organism. 产品信息 格式 Purified 控制 - Positive Control Included: Non-Stim A431 Cell Lysate (12-301). Other controls: NIH 3T3 cell lysate, HeLa cell lysate
演示 Protein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative. 应用 应用 Detect IκB-α with Anti-IκB-α Antibody (Rabbit Polyclonal Antibody), that has been shown to work in IP & WB. 主要应用 - Western Blotting
生物信息 免疫原品种 Purified GST-fusion protein containing full length human IκB-α/MAD-3 protein 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Recognizes IκB-α. 同种型 IgG 品种反应性 MouseHuman 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_013254
Entrez基因汇总 NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM] 基因符号 - IkappaBalpha
- NFKBIA
- I-kappa-B-alpha
- IKB-alpha
- NFKBI
- IKBA
- MAD3
- MAD-3
纯化方法 Protein A purfied UniProt编号 - P25963
UniProt汇总 FUNCTION: SwissProt: P25963 # Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. May be involved in regulation of transcriptional responses to NF-kappa-B, including cell adhesion, immune and proinflammatory responses, apoptosis, differentiation and growth. Controlled by sequential serine-phosphorylation, ubiquitination and degradation. Tyrosine-phosphorylation could only lead to dissociation from NF-kappa-B.
SIZE: 317 amino acids; 35609 Da
SUBUNIT: Interacts with p65 (RELA). Interacts with NKIRAS1 and NKIRAS2. Interacts with HBV protein X.
SUBCELLULAR LOCATION: Cytoplasm.
PTM: Phosphorylated; disables inhibition of NF-kappa-B DNA-binding activity. & Ubiquitinated; subsequent to stimulus-dependent phosphorylation on serines, polyubiquitination targets the protein for rapid degradation via the ubiquitin system.
DISEASE: SwissProt: P25963 # Defects in NFKBIA are a cause of autosomal dominant anhidrotic ectodermal dysplasia with immunodeficiency (AD-EDA-ID) [MIM:164008]. Ectodermal dysplasias (EDs) constitute a heterogeneous group of developmental disorders affecting tissues of ectodermal origin. EDs are characterized by abnormal development of two or more ectodermal structures such as hair, teeth, nails and sweat glands, with or without any additional clinical sign. Each combination of clinical features represents a different type of ectodermal dysplasia.
SIMILARITY: SwissProt: P25963 ## Belongs to the NF-kappa-B inhibitor family. & Contains 5 ANK repeats.产品使用声明 质量保证 routinely evaluated by immunoblot on RIPA lysates from A431 cells 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 包装信息 数量 200 µg
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