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品种反应性 主要应用 宿主 格式 抗体类型 M, H WB, ICC Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 06-1125 描述 Anti-Ipaf (NLRC4) Antibody Alternate Names - CARD, LRR, and NACHT-containing protein
- NLR family, CARD domain containing 4
- NOD-like receptor C4
- caspase recruitment domain family, member 12
- ICE-protease activating factor
- nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 4
- NLR family CARD domain-containing protein 4
- Caspase recruitment domain-containing protein 12
- Ice protease-activating factor
- Clan protein
背景信息 Ipaf, also known as NLRC4, is a human CED-4 homologue and is thought to be an activator of pro-caspase-1 and might also be associated with the activation of caspase-1 as a response to pro-inflammatory and apoptotic stimulus. It has been shown to be induced by p53 and contributes to p53-dependent apoptosis. Ipaf is thought to play a role in the activation of the innate immune system. 产品信息 格式 Affinity Purified 控制 - Human lung tissue lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 This Anti-Ipaf (NLRC4) Antibody is validated for use in IC, WB for the detection of Ipaf (NLRC4). 主要应用 - Western Blotting
- Immunocytochemistry
应用说明 Western Blot Analysis: 1 µg/mL of this antibody detected Ipaf in 10 µg of jurkat cell lysate. For this particular lysate, the antibody detected isoform 2 splice variant.
Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected Ipaf in NIH/3T3 cells.生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to human Ipaf (NLRC4) at the LRR4 domain. 表位 LRR4 domain 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes Ipaf at the LRR4 domain. 品种反应性 MouseHuman Species Reactivity Note Demonstrated to react with human and mouse. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_067032
Entrez基因汇总 Summary: In C. elegans, Ced4 binds and activates Ced3, an apoptotic initiator caspase, via caspase-associated recruitment domains (CARDs). Human Ced4 homologs include APAF1 (MIM 602233), NOD1/CARD4 (MIM 605980), and NOD2/CARD15 (MIM 605956). These proteins have at least 1 N-terminal CARD domain followed by a centrally located nucleotide-binding domain (NBD or NACHT) and a C-terminal regulatory domain, found only in mammals, that contains either WD40 repeats or leucine-rich repeats (LRRs). CARD12 is a member of the Ced4 family and can induce apoptosis.[supplied by OMIM]. 基因符号 - Ipaf
- IPAF
- CLAN
- CARD12
- CLAN1
- ipaf
- CLR2.1
- CLAND
- CLANA
- CLANB
- CLANC
纯化方法 Affinity Purified UniProt编号 - Q9NPP4
UniProt汇总 FUNCTION: Plays a role in the promotion of apoptosis.
SUBUNIT STRUCTURE: Self-associates and binds to ASC, pro-caspase-1, NOD2, BCL10 and NALP1 (NAC) by CARD-CARD interaction.
SUBCELLULAR LOCATION: Cytoplasm. Note: Cytoplasmic filaments.
TISSUE SPECIFICITY: Isoform 2 is expressed ubiquitously, although highly expressed in lung and spleen. Isoform 1 is highly expressed in lung, followed by leukocytes especially monocytes, lymph node, colon, brain, prostate, placenta, spleen, bone marrow and fetal liver. Isoform 4 is only detected in brain.
SEQUENCE SIMILARITIES: Contains 1 CARD domain.
Contains 14 LRR (leucine-rich) repeats.
Contains 1 NACHT domain.产品使用声明 质量保证 Evaluated by Western Blot in human lung tissue lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected Ipaf in 10 µg of human lung tissue lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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