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品种反应性 主要应用 宿主 格式 抗体类型 R, H, M ICC, WB, IHC Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 06-1063 描述 Anti-Frizzled-7 Antibody Alternate Names - frizzled-7
- frizzled homolog 7
- Frizzled, homolog of, 7
背景信息 Frizzled 7 is a multi-pass membrane protein belonging to the G-protein coupled receptor Fz/Smo family. It is a receptor for Wnt signaling, predominantly Wnt11, a crucial factor in the regulation of cellular migration and polarization during vertebrate gastrulation. Highest reported expression is in fetal kidney tissue and adult heart tissue. Lower levels have been observed in brain, placenta, adult heart, fetal lung, and squamous cell esophageal carcinomas. 产品信息 格式 Affinity Purified 控制 - Mouse brain microsomal fraction tissue lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. 应用 应用 This Anti-Frizzled-7 Antibody is validated for use in WB, IH, IC for the detection of Frizzled-7. 主要应用 - Immunocytochemistry
- Western Blotting
- Immunohistochemistry
应用说明 Western Blot Analysis: A previous lot detected Frizzled-7 on 10 µg of HEK293 cell lysate and rat brain microsomal fraction tissue lysate.
Immunohistochemistry Analysis: 1:300 dilution from a previous lot detected Frizzled-7 in human ductal in situ and GI tract tissue.
Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected Frizzled-7 in A431 cells.生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to human Frizzled-7. 表位 Unknown 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Recognizes Frizzled-7 品种反应性 RatHumanMouse Species Reactivity Note Demonstrated to react with human, mouse, and rat. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_003498.1
Entrez基因汇总 Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD7 protein contains an N-terminal signal sequence, 10 cysteine residues typical of the cysteine-rich extracellular domain of Fz family members, 7 putative transmembrane domains, and an intracellular C-terminal tail with a PDZ domain-binding motif. FZD7 gene expression may downregulate APC function and enhance beta-catenin-mediated signals in poorly differentiated human esophageal carcinomas. [provided by RefSeq]. 基因符号 - Fz-7
- fz-7
- FzE3
- hFz7
纯化方法 Affinity purified UniProt编号 - O75084
UniProt汇总 FUNCTION: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.
SUBUNIT STRUCTURE: Interacts with MAGI3 (By similarity).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: High expression in adult skeletal muscle and fetal kidney, followed by fetal lung, adult heart, brain, and placenta. Specifically expressed in squamous cell esophageal carcinomas.
DOMAIN: Lys-Thr-X-X-X-Trp motif is involved in the activation of the Wnt/beta-catenin signaling pathway (By similarity).
The FZ domain is involved in binding with Wnt ligands (By similarity).
SEQUENCE SIMILARITIES: Belongs to the G-protein coupled receptor Fz/Smo family.
Contains 1 FZ (frizzled) domain.产品使用声明 质量保证 Evaluated by Western Blot in mouse brain microsomal fraction tissue lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected Frizzled-7 on 10 µg of mouse brain microsomal fraction tissue lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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