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品种反应性 主要应用 宿主 格式 抗体类型 R, H, M, Eq ICC, WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 06-1047 描述 Anti-IRF4 Antibody, NT Alternate Names - interferon regulatory factor 4
- Lymphocyte-specific interferon regulatory factor
- Multiple myeloma oncogene
背景信息 Interferon regulatory factor 4, also known as IRF4, belongs to the IRF family. IRF4 encodes a 450 amino acid polypeptide with a predicted molecular weight of 51.6 kDa (Grossman 1996). The IRF4 gene is expressed in the spleen, lymphocytes and melanocytes, and is induced in to T cells after cross linking with the T cell receptor. IRF4 is a transcriptional activator that binds to the interferon stimulated response element (ISRE) of the MHC class I promoter. IRF4 is essential for mature T and B lymphocyte function. IRF4 interacts with MYD88 to prevent the interaction of IRF5, resulting in negative regulation of Toll Like Receptor (TLR) signaling. High levels of IRF4 have been associated with Epstein Barr Virus (EBV) and may be critical to EBV transformation. Further oncogenic potential has been associated with the involvement of IRF4 with hair color and pigmentation of the skin, which may cause multiple myeloma. 产品信息 格式 Affinity Purified 控制 - Mouse brain lysate.
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-IRF4 Antibody, N-terminus detects level of IRF4 & has been published & validated for use in WB & IC. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Immunocytochemistry: 1:500 dilution of a previous lot of this antibody was used on immunocytochemistry in PC3 cells. 生物信息 免疫原品种 KLH conjugated linear peptide corresponding to the N-terminal region of IRF4. 表位 N-Terminus 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes the N-terminal region of IRF4 at and around amino acids 177-194. 品种反应性 RatHumanMouseHorse Species Reactivity Note Tested to react with human and mouse. Predicted to react with rat and horse based on sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_002460
基因符号 - NF-EM5
- IRF-4
- LSIRF
- MUM1
纯化方法 Affinity Purfied UniProt编号 - Q15306
UniProt汇总 Function: Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells.
Subunit structure: Interacts with SPIB and DEF6. Ref.5 Ref.6
Subcellular location: Nucleus.
Tissue specificity: Lymphoid cells.
Induction: Not induced by interferons.
Involvement in disease: A chromosomal aberration involving IRF4 may be a cause of multiple myeloma [MIM:254500]. Translocation t(6;14)(p25;q32) with the IgH locus.
Sequence similarities: Belongs to the IRF family.
Contains 1 tryptophan pentad repeat DNA-binding domain.
产品使用声明 质量保证 Evaluated by western blot on mouse brain lysate.
Western Blot Analysis: 0.5 μg/mL of this antibody detected IRF4 in 10 μg of mouse brain lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8ºC from date of receipt. 包装信息 数量 100 µg
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下一件merck millipore产品：merck millipore,默克密理博,341612,InhibitorSelect™ FGF Signaling Pathway Inhibitor Panel
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