merck millipore,默克密理博,04-205,Anti-CENP-A Antibody, clone EP800Y, rabbit monoclonal

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merck millipore,默克密理博,04-205,Anti-CENP-A Antibody, clone EP800Y, rabbit monoclonal
产品名称：Anti-CENP-A Antibody, clone EP800Y, rabbit monoclonal
产品型号：04-205
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.

merck millipore,默克密理博,04-205,Anti-CENP-A Antibody, clone EP800Y, rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-205,Anti-CENP-A Antibody, clone EP800Y, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H	WB, FC, ICC, IHC	Rb	Unpurified	Monoclonal Antibody

描述
产品目录编号	04-205
品牌系列	Upstate
商名	Upstate
描述	Anti-CENP-A Antibody, clone EP800Y, rabbit monoclonal
Alternate Names	centromere protein A, 17kDa Centromere autoantigen A centromere protein A centromere protein A (17kD)
背景信息	Centromere protein A (CENP-A) is a 17 kDa centromere-specific histone variant with 62% amino acids homology to the C-terminal of histone H3. Localized in the centromere, it plays a central role in the centromere-specific chromatin formation. The depletion of histone H3 at the CENP-A binding domain suggests CENP-A to be a possible replacement for histone H3 in the packaging process of α-satellite DNA into primary chromatin structure. CENP-A is essential in the formation of specialized nucleosomes at the centromere, implicating CENP-A as a centromere-specific epigenetic marker.

产品信息
格式	Unpurified
控制	HeLa Whole Cell lysate.
演示	Unpurified rabbit monoclonal IgG in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.
主要应用	Western Blotting Flow Cytometry Immunocytochemistry Immunohistochemistry
应用说明	Flow Cytometry: 1:80 dilution of a previous lot was used for flow cytometry. Immunocytochemistry: 1:250 – 500 dilution of a previous lot was used for Immunocytochemistry. Immunohistochemistry: 1:250 – 500 dilution of a previous lot was used for Immunohistochemistry. Immunocytochemistry: A previous lot was used to detect CENP-A in HeLa cells (1:250 dilution).

生物信息
免疫原品种	Synthetic peptide corresponding to the C-terminal region of CENP-A, conjugated to KLH.
表位	C-terminus
克隆	EP800Y
宿主	Rabbit
特异性	This antibody recognizes the C-terminal region of human CENP-A.
同种型	IgG
品种反应性	RatHuman
Species Reactivity Note	Proven to react with human and rat CENP-A. Does not react with mouse.
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_001042426
Entrez基因汇总	Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. CENPA encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq]
基因符号	OTTHUMP00000122589 CENP-A
非反应性品种	Mouse
纯化方法	Unpurified
UniProt编号	P49450
UniProt汇总	FUNCTION: Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division. SUBUNIT: Forms a nucleosome-like histone octamer containing two molecules each of H2A, H2B, CENPA and H4 assembled in one CENPA-H4 heterotetramer and two H2A-H2B heterodimers. Nucleosomes containing CENPA also contain histone H2A variants such as macroH2A H2AFY and H2A.Z/H2AFZ. The CENPA-H4 heterotetramer is more compact and structurally more rigid than corresponding H3-H4 heterotetramers. Component of the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and MLF1IP/CENPU. Interacts directly with herpes virus HSV-1 ICP0 protein. Ref.5 Ref.7 Ref.12 SUBCELLULAR LOCATION: Nucleus. Centromere. Kinetochore. Note= Localizes exclusively in the kinetochore domain of centromeres. Ref.10 Ref.11 DOMAIN: The CATD (CENPA targeting domain) region is responsible for the more compact structure of nucleosomes containing CENPA and is necessary and sufficient to mediate the localization into centromeres. Ref.12 PTM: Ubiquitinated Probable. Interaction with herpes virus HSV-1 ICP0 protein, leads to its degradation by the proteasome pathway. Phosphorylation of Ser-7 by Aurora-A/STK6 and Aurora-B/STK12 during prophase is required for localization of Aurora-A/STK6 and Aurora-B/STK12 at inner centromere and is essential for kinetochore function. Initial phosphorylation during prophase is mediated by Aurora-A/STK6 and is maintained by Aurora-B/STK12. DISEASE:Antibodies against CENPA are present in sera from patients with autoimmune diseases that developed autoantibodies against centrosomal proteins. Ref.6 SIMILARITY: Belongs to the histone H3 family.

产品使用声明
质量保证	Routinely evaluated by Western Blot on HeLa Whole Cell lysate. Western Blot Analysis: A 1:5,000 dilution of this lot was used to detect CENP-A HeLa Whole Cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.