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品种反应性 主要应用 宿主 格式 抗体类型 H WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 06-1044 描述 Anti-MAVS/Cardif (CT) Antibody Alternate Names - mitochondrial anti-viral signaling protein
- mitochondrial antiviral signaling protein
- IFN-B promoter stimulator 1
- CARD adaptor inducing IFN-beta
- virus-induced signaling adapter variant 1b
- Cardif
- Interferon beta promoter stimulator protein 1
- CARD adapter inducing interferon-beta
- Putative NF-kappa-B-activating protein 031N
- interferon-beta promoter stimulator protein 1
- Virus-induced-signaling adapter
- mitochondrial antiviral-signaling protein
- CARD adapter inducing interferon beta
- virus-induced signaling adaptor variant 1a
背景信息 Mitochondrial anti-viral signaling protein (MAVS/Cardif/VISA) is thought to activate NF-KappaB and IRF 3, which increase anti-viral immunity. This result seems to point to mitochondrial involvement in innate immunity, specifically during viral infection. MAVS also seems to be involved in the activation of interferons, but only during viral contamination. It has been shown that MAVS works in conjunction with retinoic acid inducible gene I (RIG-I) to identify and remove replicating viral genomes. MAVS has been shown to be cleaved during apoptosis and viral infection, which could mean that this is an essential step of infection. 产品信息 格式 Affinity Purified 控制 - A549 cell lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-MAVS/Cardif (C-term) Antibody is an antibody against MAVS/Cardif (C-term) for use in WB & IP. 主要应用 - Western Blotting
应用说明 Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in IP (Lad, S., et al. (2008). Molecular Immunology 45:2277–2287). 生物信息 免疫原品种 GST-tagged recombinant protein corresponding to the C-terminus of human MAVS. 表位 C-terminus 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes MAVS at the C-terminus. 品种反应性 Human Species Reactivity Note Demonstrated to react with human.
Other homologies: Chipanzee (99% sequence homology). Rhesus Macaque (88% sequence homology). Rat (46% sequence homology). Mouse (44% sequence homology).抗体类型 Polyclonal Antibody Entrez基因编号 - NP_065797.2
Entrez基因汇总 Double-stranded RNA viruses are recognized in a cell type-dependent manner by the transmembrane receptor TLR3 (MIM 603029) or by the cytoplasmic RNA helicases MDA5 (MIM 606951) and RIGI (ROBO3; MIM 608630). These interactions initiate signaling pathways that differ in their initial steps but converge in the activation of the protein kinases IKKA (CHUK; MIM 600664) and IKKB (IKBKB; MIM 603258), which activate NFKB (see MIM 164011), or TBK1 (MIM 604834) and IKKE (IKBKE; MIM 605048), which activate IRF3 (MIM 603734). Activated IRF3 and NFKB induce transcription of IFNB (IFNB1; MIM 147640). For the TLR3 pathway, the intermediary molecule before the pathways converge is the cytoplasmic protein TRIF (TICAM1; MIM 607601). For RIGI, the intermediary protein is mitochondria-bound IPS1 (Sen and Sarkar, 2005 [PubMed 16239922]).[supplied by OMIM]. 基因符号 - CARDIF
- VISA
- MAVS
- IPS-1
- IPS1
纯化方法 Affinity Purified UniProt编号 - Q7Z434
UniProt汇总 FUNCTION: Required for innate immune defense against viruses. Acts downstream of DDX58 and IFIH1/MDA5, which detect intracellular dsRNA produced during viral replication, to coordinate pathways leading to the activation of NF-kappa-B, IRF3 and IRF7, and to the subsequent induction of antiviral cytokines such as IFN-beta and RANTES (CCL5). May activate the same pathways following detection of extracellular dsRNA by TLR3. May protect cells from apoptosis.
SUBUNIT STRUCTURE: Interacts with DDX58, IFIH1, TRAF2 and TRAF6. May interact with IRF3, FADD, RIPK1, IKBKE, CHUK and IKBKB. Does not interact with TBK1. Interacts with and is cleaved by HCV and hepatitis GB virus B NS3/4A proteases. Interacts with and is cleaved by HHAV protein 3ABC. Interacts with NLRX1. Interaction with NLRX1 requires the CARD domain. Interacts with PSMA7. Interacts with TRAFD1 By similarity. Interacts (via C-terminus) with PCBP2 in a complex containing MAVS, PCBP2 and ITCH. Interacts with CYLD.
SUBCELLULAR LOCATION: Mitochondrion outer membrane
TISSUE SPECIFICTY: Present in T-cells, monocytes, epithelial cells and hepatocytes (at protein level). Ubiquitously expressed, with highest levels in heart, skeletal muscle, liver, placenta and peripheral blood leukocytes.
DOMAIN: Both CARD and transmembrane domains are essential for antiviral function. The CARD domain is responsible for interaction with DDX58 and IFIH1.
PTM: Ubiquitinated; undergoes 'Lys-48'-linked polyubiquitination catalyzed by ITCH; ITCH-dependent polyubiquitination is mediated by the interaction with PCBP2 and leads to MAVS proteosomal degradation.
MISCELLANEOUS: Cleavage by HCV protease complex leads to inactivation.
SEQUENCE SIMILARITIES: Contains 1 CARD domain.
SEQUENCE CAUTION: The sequence BAA86585.1 differs from that shown. Reason: Erroneous initiation.
The sequence BAB14684.1 differs from that shown. Reason: Frameshift at position 333.产品使用声明 质量保证 Evaluated by Western Blot in A549 cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected MAVS in 10 µg of A549 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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