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品种 宿主细胞 蛋白靶 Human Chem-1 FP 描述 产品目录编号 HTS093RTA 商名 - Ready-to-Assay
描述 Ready-to-Assay™ FP Prostanoid Receptor Frozen Cells 背景信息 Millipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.
Prostanoids are a series of arachidonic acid metabolites produced by the action of cyclooxygenase and subsequently by isomerases and synthases. Cells rapidly secrete prostanoids after synthesis, whereupon the prostanoids bind to a family of 8 GPCRs to exert their biological effects (Narumiya and FitzGerald, 2001). The prostaglandin PGF2alpha binds specifically to the FP receptor, which couples to Gq/11 to mobilize intracellular calcium. Binding of PGF2alpha to FP receptors in the corpus luteum is required for luteolysis and subsequent parturition in mice (Sugimoto et al., 1998). PGF2alpha also decreases intraocular pressure by an FP-dependent mechanism, and an PGF2--analog--latanoprost, is used clinically in the treatment of glaucoma (Crowston et al., 2004). FP also contributes to tachycardia induced by inflammatory stimuli (Takayama et al., 2005). Chemicon's cloned human FP-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant FP expression on the cell surface and contains high levels of the promiscuous G protein Galpha15 to enhance coupling of the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between FP and its ligands.产品信息 部件 - Fifty (50) mL of Media Component.
- Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
应用 应用 Human FP GPCR Frozen Cells for calcium flux FLIPR Assays. 生物信息 品种 Human GPCR类别 A 宿主细胞 Chem-1 蛋白靶 FP 靶子系列 Prostanoid 细胞系类型 - Ready-to-Assay
产品使用声明 销售限制 This product requires a User Agreement (Label License). Please see product datasheet for further information. 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
- This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled.
储存和货运信息 存储条件 Place cells in liquid nitrogen immediately upon receipt. 包装信息 数量 1 kit
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