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merck millipore,默克密理博,06-1040,Anti-RIG-I (NT) Antibody
产品名称：Anti-RIG-I (NT) Antibody
产品型号：06-1040
Use Anti-RIG-I (N-Terminus) Antibody (Rabbit Polyclonal Antibody) validated in WB, IP to detect RIG-I (N-Terminus) also known as Retinoic acid-inducible gene 1 protein.
merck millipore,默克密理博,06-1040,Anti-RIG-I (NT) Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 06-1040 描述 Anti-RIG-I (NT) Antibody Alternate Names - RIG-I
- Probable ATP-dependent RNA helicase DDX58
- DEAD box protein 58
- Retinoic acid-inducible gene 1 protein
- RIG-1
- Retinoic acid-inducible gene I protein
背景信息 Retinoic acid-inducible gene 1 protein (RIG-1) is a cytoplasmic DExD/H box RNA helicase belonging to the helicase family and containing 2 CARD domains within the N-terminus, a repressor domain (RD), a helicase ATP-binding domain and a helicase C-terminal binding domain. RIG-1 is able to bind the 5’-triphosphate RNA of single or double-stranded viral RNA. After recognizing and binding viral RNA, RIG-1 protein initializes type I IFN production, and within fibroblasts and dendritic cells plays a central role in the regulation of type I IFN production in response to viral presence. There are four known isoforms resulting from alternative splice variants. 产品信息 格式 Affinity Purified 控制 - SeV treated and untreated A549 cell lysates
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Use Anti-RIG-I (N-Terminus) Antibody (Rabbit Polyclonal Antibody) validated in WB, IP to detect RIG-I (N-Terminus) also known as Retinoic acid-inducible gene 1 protein. 主要应用 - Western Blotting
应用说明 Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP. (Paz, S., et al. (2011). Cell Research. 1-16.) 生物信息 免疫原品种 GST-tagged recombinant protein corresponding to the N-terminus of human RIG-I. 表位 N-terminus 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes RIG-I at the N-terminus. 品种反应性 Human Species Reactivity Note Demonstrated to react with Human. Other homologies: Rhesus Monkey (99% sequence homology). 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_055129.2
Entrez基因汇总 DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. This gene encodes a protein containing RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of immune response. [provided by RefSeq]. 基因符号 - DDX58
纯化方法 Affinity purified UniProt编号 - O95786
UniProt汇总 FUNCTION: Involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). Essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV (By similarity).
CATALYTIC ACTIVITY: ATP + H2O = ADP + phosphate.
COFACTOR: Zinc.
SUBUNIT STRUCTURE: Monomer; maintained as a monomer in an autoinhibited state. Upon viral dsRNA binding and conformation shift, homomultimerizes and interacts with MAVS. Interacts with DHX58/LGP2, IKBKE, TBK1 and TMEM173/STING. Interacts (via CARD domain) with TRIM25 (via SPRY domain). Interacts with RNF135. Interacts with CYLD. Interacts with NLRC5; blocks the interaction of MAVS to DDX58.
SUBCELLULAR LOCATION: Cytoplasm. Note: Colocalized with TRIM25 at cytoplasmic perinuclear bodies.
TISSUE SPECIFICTY: Present in vascular smooth cells (at protein level).
INDUCTION: By bacterial lipopolysaccharides (LPS) in endothelial cells. By IFN-alphas, IFNB1/IFN-beta and IFNG/IFN-gamma.
DOMAIN: The repressor domain controls homomultimerization and interaction with MAVS Probable.
The helicase domain is responsible for dsRNA recognition.
The 2 CARD domains are responsible for interaction with and signaling through MAVS.
The second CARD domain is the primary site for 'Lys-63'-linked ubiquitination.
PTM: Isgylated. Conjugated to ubiquitin-like protein ISG15 upon IFN-beta stimulation.
Ubiquitinated. Undergoes 'Lys-63'-linked ubiquitination. Lys-172 is the critical site for TRIM25-mediated ubiquitination, for MAVS binding and to induce anti-viral signal transduction. Lys-154, Lys-164 and Lys-172 are critical sites for RNF135-mediated ubiquitination. Deubiquitinated by CYLD, a protease that selectively cleaves 'Lys-63'-linked ubiquitin chains.
SEQUENCE SIMILARITIES: Belongs to the helicase family.
Contains 2 CARD domains.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.产品使用声明 质量保证 Evaluated by Western Blot in SeV treated and untreated A549 cell lysates.
Western Blot Analysis: 0.05 µg/mL of this antibody detected RIG-I on 10 µg of SeV untreated and treated A549 cell lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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