merck millipore,默克密理博,17-10099,ChIPAb+ Acetyl-Histone H4 (Lys8)

搜索"Merck Millipore 密理愽"产品

Merck Millipore 默克密理愽

当前位置：首页 > >Merck Millipore > 生命科学研究
生命科学研究

merck millipore,默克密理博,17-10099,ChIPAb+ Acetyl-Histone H4 (Lys8) - ChIP Validated Antibody and Primer Set
产品名称：ChIPAb+ Acetyl-Histone H4 (Lys8) - ChIP Validated Antibody and Primer Set
产品型号：17-10099
The ChIPAb+ Acetyl-Histone H4 (Lys8) set includes the Acetyl-Histone H4 (Lys8) antibody, a negative control rabbit serum & qPCR primers which amplify a 134 bp region of human HSPCA.

merck millipore,默克密理博,17-10099,ChIPAb+ Acetyl-Histone H4 (Lys8) - ChIP Validated Antibody and Primer Set

产品介绍

merck millipore,默克密理博,17-10099,ChIPAb+ Acetyl-Histone H4 (Lys8) - ChIP Validated Antibody and Primer Set

重要规格表

品种反应性	主要应用
Yeast (S. cerevisiae), H	ChIP, WB

描述
产品目录编号	17-10099
品牌系列	Upstate
商名	Upstate ChIPAb+
描述	ChIPAb+ Acetyl-Histone H4 (Lys8) - ChIP Validated Antibody and Primer Set
概述	All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction. The ChIPAb+ Acetyl-Histone H4 (Lys8) set includes the Acetyl-Histone H4 (Lys8) antibody, a negative control rabbit serum, and qPCR primers which amplify a 134 bp region of human HSPCA. The Acetyl-Histone H4 (Lys8) and negative controls are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of Acetyl-Histone H4 (Lys8)-associated chromatin.
Alternate Names	Histone H4 (acetyl K8) H4K8Ac
背景信息	Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H4 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

产品信息
格式	Serum
控制	Includes negative control normal rabbit serum antibody and primers specific for human HSPCA.
演示	Anti-Acetyl-Histone H4 (Lys8) (rabbit polyclonal). One vial containing 50 μL of antiserum containing 0.05% sodium azide. Store at -20°C. Normal Rabbit Serum. One vial containing 100 μL of antiserum containing 0.05% sodium azide. Store at -20°C. ChIP Primers, HSPCA. One vial containing 75 μL of 5 μM of each primer specific for human HSPCA. Store at -20°C. FOR: GCA ACA GCT ACC ACA GGA CCA REV: GAG CGT GTG AAA TCA ACA TAA AGC

应用
应用	The ChIPAb+ Acetyl-Histone H4 (Lys8) set includes the Acetyl-Histone H4 (Lys8) antibody, a negative control rabbit serum & qPCR primers which amplify a 134 bp region of human HSPCA.
主要应用	Chromatin Immunoprecipitation (ChIP) Western Blotting
应用说明	Chromatin Immunoprecipitation: Sonicated chromatin prepared from HeLa cells (1 X 10⁶ cell equivalents per IP) were subjected to chromatin immunoprecipitation using either 2 µL of Normal Rabbit Serum or 2 µL of Anti-Acetyl-Histone H4 (Lys8) and the Magna ChIP™ A Kit (Cat. # 17-610). Successful immunoprecipitation of Acetyl-Histone H4 (Lys8) associated DNA fragments was verified by qPCR using ChIP Primers, human HSPCA for a positive, and known Negative Primers (Martinato, F., et al., 2008) as a negative assay (Please see figures). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated. Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details. Western Blot Analysis: Representative lot data. Sodium Butyrate-treated HeLa acid extract was resolved by electrophoresis, transferred to PVDF membrane and probed with Anti-Acetyl Histone H4 (Lys8) (1:1000 dilution). To demonstrate specificity, serum was preincubated with modified Histone H4 peptides: Lane 1: No peptide Lane 2: acetyl-Histone H4 (K5) (Cat.#:12-343) Lane 3: acetyl-Histone H4 (K8) (Cat.#:12-344) Lane 4: acetyl-Histone H4 (K12) (Cat.#:12-345) Lane 5: acetyl-Histone H4 (K16) (Cat.#:12-346) Lane 6: acetyl-Histone H4 (K5, 8, 12, 16) (Cat.#:12-353) Lane 7: Histone H4 (Cat.#:12-347) Proteins were visualized using a donkey anti-rabbit IgG conjugated to HRP and visualized using a chemiluminescence detection system (Please see figures). Immunoprecipitation: 5 μL of a previous lot immunoprecipitated acetyl Histone H4 from 1 mg of wild type whole cell yeast lysates but not from a yeast strain containing a Histone H4 Lys8R substitution.

生物信息
免疫原品种	Synthetic peptide corresponding to yeast Histone H4 acetylated at lysine 8.
宿主	Rabbit
特异性	Recognizes acetyl-Histone H4 (Lys8).
品种反应性	Yeast (S. cerevisiae)Human
Species Reactivity Note	Demonstrated to react with Yeast and human. Broad species cross-reactivity is expected based on 100% sequence homology.
抗体类型	Polyclonal Antibody
Entrez基因编号	NM_175054
Entrez基因汇总	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
基因符号	H4/E HIST1H4A H4/J HIST1H4F HIST1H4L H4FN H4FA H4FH HIST1H4H H4F2 H4FM H4/A H4FK H4FG HIST2H4 H4FB H4/K HIST1H4I H4/N HIST1H4B H4FE H4/M H4/a HIST1H4E HIST4H4 HIST2H4A H4FC H4FI H4/H HIST1H4C H4/G HIST1H4K H4FJ H4FD H4/I H4/B H4/D H4/C HIST1H4J HIST1H4D
修改	Acetylation
纯化方法	Unpurified
UniProt编号	P62805
UniProt汇总	FUNCTION: SwissProt: P62805 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. SIZE: 103 amino acids; 11367 Da SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. SUBCELLULAR LOCATION: Nucleus. PTM: Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage (By similarity). & Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. SIMILARITY: SwissProt: P62805 ## Belongs to the histone H4 family.

产品使用声明
质量保证	Chromatin Immunoprecipitation: Sonicated chromatin prepared from HeLa cells (1 X 10⁶ cell equivalents per IP) were subjected to chromatin immunoprecipitation using either 2 µL of Normal Rabbit Serum or 2 µL of Anti-Acetyl-Histone H4 (Lys8) and the Magna ChIP™ A Kit (Cat. # 17-610). Successful immunoprecipitation of Acetyl-Histone H4 (Lys8) associated DNA fragments was verified by qPCR using ChIP Primers, human HSPCA (Please see figures). Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品使用声明

质量保证

Chromatin Immunoprecipitation:
Sonicated chromatin prepared from HeLa cells (1 X 10⁶ cell equivalents per IP) were subjected to chromatin immunoprecipitation using either 2 µL of Normal Rabbit Serum or 2 µL of Anti-Acetyl-Histone H4 (Lys8) and the Magna ChIP™ A Kit (Cat. # 17-610).
Successful immunoprecipitation of Acetyl-Histone H4 (Lys8) associated DNA fragments was verified by qPCR using ChIP Primers, human HSPCA (Please see figures).
Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.