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Replacement Information Replacement Information 05-14013 is a recommended replacement for MAB3077 重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Gp, R, B, M, H WB M Purified Monoclonal Antibody 描述 产品目录编号 05-1403 Replaces MAB3077 描述 Anti-Giα2 Antibody, clone L5 产品信息 格式 Purified 控制 - Huvec cell lysate
演示 Purified mouse monoclonal IgG2b, in 0.02 M phosphate buffer, 0.25 M sodium chloride, pH 7.6, with 0.1% sodium azide. 应用 应用 This Anti-Giα2 Antibody, clone L5 is validated for use in WB, IH(P) for the detection of Giα2. 主要应用 - Western Blotting
应用说明 Optimal working dilutions must be determined by end user.
Immunohistochemistry(paraffin): Representative testing from a previous lot.
Optimal Staining of G-Protein Monoclonal Antibody: Testicular Cancer
Western Blot: A previous lot of this antibody was used at 1:500 - 1:1000, with detection by 125l-labeled anti-IgG. Labels a single protein band of 39 - 42 kDa in bovine and rat membranes生物信息 免疫原品种 Recombinant Giα-2, purified by the method of Linder (J. Biol. Chem. [1990] 265, 8243). 克隆 L5 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 This antibody recognizes Giα-2. 同种型 IgG2b 品种反应性 Guinea PigRatBovineMouseHuman Species Reactivity Note Strong reactivity with bovine (100%), mouse (69%), rat (69%), human (62%) and guinea pig (43%). Specificity confirmed by Western blot versus bovine Gαt, rat Goα, rat Giα-1, Giα-2, rat Gia-3, bovine Gsα, and murine GoαA and GoαB. 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_002070.2
基因符号 - WUGSC:H_LUCA16.1
- GNAI2
- GNAI2B
- WUGSC:H_LUCA15.1
- H_LUCA15.1
- H_LUCA16.1
- GIP
纯化方法 Purified UniProt编号 - P04899
UniProt汇总 FUNCTION: SwissProt: P04899 # Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli.
SIZE: 355 amino acids; 40451 Da
SUBUNIT: G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts with GPSM1 (By similarity). Interacts with UNC5B.
SIMILARITY: SwissProt: P04899 ## Belongs to the G-alpha family. G(i/o/t/z) subfamily.产品使用声明 质量保证 Routinely evaluated by Western Blot on Huvec lysates.
Western Blot Analysis: 1:500 dilution of this lot detected G I ALPHA 2 on 10 μg of Huvec lysates.
使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at 2-8°C for 1 year from date of receipt. 包装信息 数量 100 µg
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