merck millipore,默克密理博,05-829,Anti-α-Tubulin Antibody, clone DM1A

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merck millipore,默克密理博,05-829,Anti-α-Tubulin Antibody, clone DM1A
产品名称：Anti-α-Tubulin Antibody, clone DM1A
产品型号：05-829
Anti-α-Tubulin Antibody, clone DM1A is an antibody against α-Tubulin for use in IC, IF & WB.

merck millipore,默克密理博,05-829,Anti-α-Tubulin Antibody, clone DM1A

产品介绍

merck millipore,默克密理博,05-829,Anti-α-Tubulin Antibody, clone DM1A

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
H, R, M, Po, B, Av, Gp	WB, ICC	M	Purified	Monoclonal Antibody

描述
产品目录编号	05-829
Replaces	04-1117
品牌系列	Upstate
商名	Upstate
描述	Anti-α-Tubulin Antibody, clone DM1A
Alternate Names	tubulin, alpha, brain-specific Alpha-tubulin 3 Tubulin B-alpha-1 Tubulin alpha-3 chain tubulin, alpha 1a tubulin, alpha 3 alpha 3 alpha
背景信息	Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein which consists of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase ads a C-terminal tyrosin to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.

产品信息
格式	Purified
控制	A431 cell lysate.
演示	Purified mouse monoclonal IgG1 in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide and 30% glycerol.

应用
应用	Anti-α-Tubulin Antibody, clone DM1A is an antibody against α-Tubulin for use in IC, IF & WB.
主要应用	Western Blotting Immunocytochemistry
应用说明	Immunofluorescence: (Breitling, F., 1986)

生物信息
免疫原品种	Purified human brain α-Tubulin. The epitope recognized by this antibody has been mapped to amino acids 426-450 (AALEKDYEEVGVDSVEGEGEEEGEE).
表位	a.a. 426-450
克隆	DM1A
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Mouse
特异性	Recognizes α-Tubulin, clone DM1A, MW 50-60 kDa. The epitope recognized by this antibody has been mapped to amino acids 426-450.
同种型	IgG1
品种反应性	HumanRatMousePigBovineAvianGuinea Pig
Species Reactivity Note	Human, mouse, rat, bovine, porcine, guinea pig, avian.
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_006000
Entrez基因汇总	Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q.
基因符号	hum-a-tub1 TUBA1A hum-a-tub2 FLJ25113 B-ALPHA-1 TUBA3
纯化方法	Protein G purfied
UniProt编号	Q71U36
UniProt汇总	FUNCTION: SwissProt: Q71U36 # Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. SIZE: 451 amino acids; 50136 Da SUBUNIT: Dimer of alpha and beta chains. PTM: Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively (By similarity). SIMILARITY: SwissProt: Q71U36 ## Belongs to the tubulin family.

产品使用声明
质量保证	Routinely evaluated by western blot on non-stimulated A431 lysate. Western Blot Analysis: 0.1-1 µg/mL of this lot detected α- Tubulin in RIPA lysates from A431, L6 cells and rat whole brain tissue.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.