merck millipore,默克密理博,05-677,Anti-phospho-Src (Tyr416) Antibody, clone 9A6

搜索"Merck Millipore 密理愽"产品

Merck Millipore 默克密理愽

当前位置：首页 > >Merck Millipore > 生命科学研究
生命科学研究

merck millipore,默克密理博,05-677,Anti-phospho-Src (Tyr416) Antibody, clone 9A6
产品名称：Anti-phospho-Src (Tyr416) Antibody, clone 9A6
产品型号：05-677
Detect phospho-Src (Tyr416) using this Anti-phospho-Src (Tyr416) Antibody, clone 9A6 validated for use in WB & IP.

merck millipore,默克密理博,05-677,Anti-phospho-Src (Tyr416) Antibody, clone 9A6

产品介绍

merck millipore,默克密理博,05-677,Anti-phospho-Src (Tyr416) Antibody, clone 9A6

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
M, H	WB	M	Purified	Monoclonal Antibody

描述
产品目录编号	05-677
品牌系列	Upstate
商名	Upstate
描述	Anti-phospho-Src (Tyr416) Antibody, clone 9A6
概述	Specific src isoform is unknown
Alternate Names	v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) proto-oncogene tyrosine-protein kinase SRC protooncogene SRC, Rous sarcoma tyrosine kinase pp60c-src tyrosine-protein kinase SRC-1 v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog
背景信息	Src (also known as pp60src) is a non receptor Tyrosine Kinase involved in signal transduction in many biological systems and implicated in the development of human tumors. There are two critical phosphorylation sites of tyrosine on Src, tyrosine 418 and tyrosine 529 (referring to human Src sequence). The tyrosine 418 is located in the catalytic domain and is one of the autophosphorylation sites. Full catalytic activity of Src requires phosphorylation of tyrosine 418. This region of Src is also highly conserved in all of the related Src-family kinases, and thus prior immunoprecipitation may be required to identify which Src family member is being activated.

产品信息
格式	Purified
控制	Chick embryo fibroblast (CEF) cells expressing human wild-type Src protein
演示	Purified mouse monoclonal IgG3 lyophilized from 1 mL 2x PBS / 0.09 % Na-azide / PEG and Sucrose. Reconstitute with 1 mL H2O (15 min, RT). Lyophilized. Store at -20ºC.

应用
应用	Detect phospho-Src (Tyr416) using this Anti-phospho-Src (Tyr416) Antibody, clone 9A6 validated for use in WB & IP.
主要应用	Western Blotting
应用说明	Immunoprecipitation/Immunoblot Analysis: 4 μg/mL of a previous lot detected phospho-Src immunoprecipitated from 3T3 cell lysates with 10 μg of anti-Src (Catalog # 05-184), but not after phosphatase treatment with YOP.

生物信息
免疫原品种	KLH-conjugated, synthetic peptide containing phosphotyrosine in the sequence context corresponding to tyrosine 416 of avian Src. Clone 9A6.
克隆	clone 9A6
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Mouse
特异性	Recognizes phospho-Src, Mr 60kDa. The antibody does not crossreact with the nonphosphorylated form of Src or with unrelated phosphorylation sites. Predicted to recognize all Src family members phosphorylated at the tyrosine corresponding to Tyr416 of avian Src.
同种型	IgG3
品种反应性	MouseHuman
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_005417
Entrez基因汇总	This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
基因符号	pp60c-src SRC c-src p60-Src SRC1 c-SRC ASV
修改	Phosphorylation
纯化方法	Thiophilic adsoption and size exclusion chromatography
UniProt编号	P12931
UniProt汇总	SIZE: 536 amino acids; 59835 Da SUBUNIT: Interacts with DDEF1/ASAP1 via its SH3 domain. Interacts with CCPG1 (By similarity). Interacts with CDCP1, PELP1, TGFB1I1 and TOM1L2. PTM: Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. The phosphorylated tail interacts with the SH2 domain thereby repressing kinase activity. SIMILARITY: SwissProt: P12931 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. & Contains 1 protein kinase domain. & Contains 1 SH2 domain. & Contains 1 SH3 domain.

产品使用声明
质量保证	Evaluated by immunoprecipitating Src from 3T3 cell lysates with anti-Src (Catalog #05-184), but not after phosphatase treatment with YOP. Src Autophosphorylation/Western Blotting Analysis: 0.5-2 μg/mL of this antibody strongly detected recombinant Src (Catalog # 14-326) after incubation with Mg/Mn/ATP. Phospho-specificity was confirmed by dephosphorylation of the Src protein with λ-phosphatase.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品使用声明

质量保证

Evaluated by immunoprecipitating Src from 3T3 cell lysates with anti-Src (Catalog #05-184), but not after phosphatase treatment with YOP.

Src Autophosphorylation/Western Blotting Analysis: 0.5-2 μg/mL of this antibody strongly detected recombinant Src (Catalog # 14-326) after incubation with Mg/Mn/ATP. Phospho-specificity was confirmed by dephosphorylation of the Src protein with λ-phosphatase.

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.