merck millipore,默克密理博,07-645,Anti-Sp1 Antibody

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merck millipore,默克密理博,07-645,Anti-Sp1 Antibody
产品名称：Anti-Sp1 Antibody
产品型号：07-645
Use Anti-Sp1 Antibody (Rabbit Polyclonal Antibody) validated in ChIP, EMSA, IF, IP, WB to detect Sp1 also known as Sp1 transcription factor, specificity protein 1.

merck millipore,默克密理博,07-645,Anti-Sp1 Antibody

产品介绍

merck millipore,默克密理博,07-645,Anti-Sp1 Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	ChIP, EMSA, WB	Rb	Purified	Polyclonal Antibody

描述
产品目录编号	07-645
Replaces	MABE550
品牌系列	Upstate
商名	Upstate
描述	Anti-Sp1 Antibody
Alternate Names	specificity protein 1 Sp1 transcription factor
背景信息	Sp1 is a human transcription factor involved in gene expression in the early development of an organism. The protein is 785 amino acids long, with a molecular weight of 95 kDA. The SP1 transcription factor contains a zinc finger protein motif, by which it binds directly to DNA and enhances gene transcription. Sp1 has always been described as a ubiquitous transcription factor that is required for the constitutive and inducible expression of a variety of genes, such as in cell cycle or mammalian development.

产品信息
格式	Purified
控制	HeLa nuclear extract.
演示	Purified rabbit polyclonal IgG in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide and 30% glycerol.

应用
应用	Use Anti-Sp1 Antibody (Rabbit Polyclonal Antibody) validated in ChIP, EMSA, IF, IP, WB to detect Sp1 also known as Sp1 transcription factor, specificity protein 1.
主要应用	Chromatin Immunoprecipitation (ChIP) Electrophoretic Mobility Shift Assay Western Blotting
应用说明	Immunoprecipitation: 4 µg of this lot immunoprecipitated Sp1 from 500 µg of HeLa nuclear extract. NOTE: A previous lot successfully immunoprecipitated the α-1 subunit using the Catch and Release v2.0 Reversible Immunoprecipitation System (Catalog # 17-500). Chromatin Immunoprecipitation: Reported by an independent laboratory. Immunofluorescence: Reported by an independent laboratory. Electrophoretic Mobility Shift Assay (EMSA): 1 µL of a previous lot of this antibody has been reported by an independent laboratory to supershift DNA complexes containing recombinant Sp1 and endogenous Sp1 from HeLa cells in EMSA.

生物信息
免疫原品种	Full length human Sp1 protein.
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
特异性	Recognizes Sp1, Mr 105 kDa and 95 kDa.
同种型	IgG
品种反应性	RatHumanMouse
Species Reactivity Note	Human and mouse. Expected to cross react with rat.
抗体类型	Polyclonal Antibody
Entrez基因编号	NP_003100.1
基因符号	TSFP1
纯化方法	Protein A purfied
UniProt编号	P08047
UniProt汇总	FUNCTION: SwissProt: P08047 # Binds to GC box promoters elements and selectively activates mRNA synthesis from genes that contain functional recognition sites. Can interact with G/C-rich motifs from serotonin receptor promoter. SIZE: 785 amino acids; 80693 Da SUBUNIT: Interacts with ATF7IP, ATF7IP2, POGZ, HCFC1, AATF and PHC2. Interacts with varicella-zoster virus IE62 protein and HIV-1 Vpr. SUBCELLULAR LOCATION: Nucleus. PTM: O-glycosylated; contains N-acetylglucosamine side chains. SIMILARITY: SwissProt: P08047 ## Belongs to the Sp1 C2H2-type zinc-finger protein family. & Contains 3 C2H2-type zinc fingers.

产品使用声明
质量保证	Routinely evaluated by Western Blot on HeLa lysates. Western Blot Analysis: 1:500 dilution of this lot detected SP1 on 10 μg of HeLa lysates.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.