merck millipore,默克密理愽
  • merck millipore,默克密理博,05-593,Anti-α-Dystroglycan Antibody, clone IIH6C4

  • merck millipore,默克密理博,05-593,Anti-α-Dystroglycan Antibody, clone IIH6C4

    产品名称：Anti-α-Dystroglycan Antibody, clone IIH6C4
    产品型号：05-593
    This Anti-α-Dystroglycan Antibody, clone IIH6C4 is validated for use in IH, FUNC, WB for the detection of α-Dystroglycan.

    merck millipore,默克密理博,05-593,Anti-α-Dystroglycan Antibody, clone IIH6C4

  • 产品介绍
  • merck millipore,默克密理博,05-593,Anti-α-Dystroglycan Antibody, clone IIH6C4

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    Gp, H, R, M, Rb, CaIHC, WBMAscitesMonoclonal Antibody
    描述
    产品目录编号 05-593
    品牌系列 Upstate
    商名
    • Upstate
    描述 Anti-α-Dystroglycan Antibody, clone IIH6C4
    概述 This product may be used for research purposes only. Diagnostic use of this product requires a license from the University of Iowa Research Foundation, 214 Technology Innovation Center, Iowa City, IA 52242
    Alternate Names
    • Large glycan
    • LARGE-glycan
    • dystrophin-associated glycoprotein-1
    • dystroglycan 1 (dystrophin-associated glycoprotein 1)
    • dystroglycan 1
    • Dystrophin-associated glycoprotein 1
    背景信息 Dystroglycans are essential elements of the neuromuscular junction (NMJ). The gene for dystroglycan is expressed as a precursor protein that is post translationally cleaved into a 156 kDa extracellular peripheral membrane protein called alpha dystroglycan and a 43 kDa transmembrane protein, beta dystroglycan. The latter protein contains a PPxY motif that promotes binding to WW domain containing proteins, such as utrophin and dystrophin. Phosphorylation at tyrosine 892 within the PPxY motif may regulate c Src interactions with beta dystroglycan, as well as inhibit interactions with WW domain proteins. In skeletal muscle, beta dystroglycan is normally localized to the plasma membrane, however phosphorylation of Tyr892 leads to localization of beta dystroglycan to endosomal compartments along with c Src. Thus, phosphorylation at Tyr892 may have important roles in altering the localization of beta dystroglycan during NMJ formation.
    产品信息
    格式 Ascites
    控制
    • Rabbit skeletal muscle lysate.
    演示 Mouse ascites, in PBS containing 0.05% sodium azide and 30% glycerol.
    Liquid at -20ºC.
    应用
    应用 This Anti-α-Dystroglycan Antibody, clone IIH6C4 is validated for use in IH, FUNC, WB for the detection of α-Dystroglycan.
    主要应用
    • Immunohistochemistry
    • Western Blotting
    应用说明 Inhibition of Laminin Binding to Dystroglycan: An independent lab has shown, in a nitrocellulose overlay experiment, that this antibody inhibits binding of 125I-laminin to dystroglycan (Ervasti, J., et al. (1993).

    Western Blot Analysis: A previous lot of this antibody was used on mouse muscle tissue lysate 3 months after shRNA induction, and in littermate controls (ctrl) and LARGE-null negative control (myd) (Goddeeris, M., et al. 2013, Nature).

    Immunofluorescence: A previous lot of this antibody was used to detect α-Dystroglycan /LARGE-glycan in mouse muscle tissue (Goddeeris, M., et al. 2013, Nature).
    生物信息
    免疫原品种 Rabbit skeletal muscle membrane preparation. Clone IIH6C4.
    克隆 IIH6C4
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Mouse
    特异性 This antibody recognizes α-Dystroglycan/LARGE-glycan, Mr 156 kDa.
    同种型 IgM
    品种反应性 Guinea PigHumanRatMouseRabbitCanine
    Species Reactivity Note Rabbit, human, mouse, rat, dog, and guinea pig.
    抗体类型 Monoclonal Antibody
    Entrez基因编号
    • NM_004393
    Entrez基因汇总 Dystroglycan is a laminin binding component of the dystrophin-glycoprotein complex which provides a linkage between the subsarcolemmal cytoskeleton and the extracellular matrix. Dystroglycan 1 is a candidate gene for the site of the mutation in autosomal recessive muscular dystrophies. The dramatic reduction of dystroglycan 1 in Duchenne muscular dystrophy leads to a loss of linkage between the sarcolemma and extracellular matrix, rendering muscle fibers more susceptible to necrosis. Dystroglycan also functions as dual receptor for agrin and laminin-2 in the Schwann cell membrane. The muscle and nonmuscle isoforms of dystroglycan differ by carbohydrate moieties but not protein sequence.
    基因符号
    • 156DAG
    • DAG1
    • A3a
    • DAG
    • beta-dystroglycan
    • alpha-dystroglycan
    • AGRNR
    纯化方法 Unpurified
    UniProt编号
    • Q14118
    UniProt汇总 FUNCTION: SwissProt: Q14118 # Forms part of the dystrophin-associated protein complex (DAPC) which may link the cytoskeleton to the extracellular matrix. Alpha-dystroglycan functions as a laminin receptor. Binds to several types of arenaviruses. Is a target for the entry of Mycobacterium leprae into peripheral nerve Schwann cells.
    SIZE: 895 amino acids; 97581 Da
    SUBUNIT: Interacts with SGCD (By similarity). Interacts with AGR2 and AGR3.
    SUBCELLULAR LOCATION: Alpha-dystroglycan: Secreted, extracellular space. & Beta-dystroglycan: Cell membrane; Single- pass type I membrane protein.
    TISSUE SPECIFICITY: Expressed in a variety of fetal and adult tissues.
    产品使用声明
    质量保证 Routinely evaluated by western blot on rabbit skeletal muscle.

    Western Blot Analysis:
    A 1:1000-1:2000 dilution of this lot detected α-Dystroglycan/LARGE-glycan in rabbit skeletal muscle.
    Note: The use of WGA purified protein results in significantly cleaner blots and immunoprecipitates.
    Post-translational modification of dystroglycan causes band broadening.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap.
    包装信息
    数量 200 µL

    merck millipore,默克密理博,05-593,Anti-α-Dystroglycan Antibody, clone IIH6C4

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