• merck millipore,默克密理博,16-213,Anti-Myc Tag Antibody, clone 4A6, HRP conjugate
  • merck millipore,默克密理博,16-213,Anti-Myc Tag Antibody, clone 4A6, HRP conjugate

    产品名称:Anti-Myc Tag Antibody, clone 4A6, HRP conjugate
    产品型号:16-213
    Anti-Myc Tag Antibody, clone 4A6, HRP conjugate detects level of Myc Tag & has been published & validated for use in WB.

    merck millipore,默克密理博,16-213,Anti-Myc Tag Antibody, clone 4A6, HRP conjugate

  • 产品介绍
  • merck millipore,默克密理博,16-213,Anti-Myc Tag Antibody, clone 4A6, HRP conjugate

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    HWBMHRPMonoclonal Antibody
    描述
    产品目录编号 16-213
    品牌系列 Upstate
    商名
    • Upstate
    描述 Anti-Myc Tag Antibody, clone 4A6, HRP conjugate
    概述 Immunogen: peptide corresponding to amino acids 410-420 (MEQKLISEEDL) of human Myc

    Specificity: Recognizes and is specific for recombinant proteins containing the Myc epitope tag (EQKLISEEDL) in a variety of sequence contexts. Also recognizes human Myc.

    Species Cross-reactivity: Human, others not tested

    Ig Type: IgG

    Host: Mouse

    Molecular Weight: Varies depending upon the protein which is tagged
    Alternate Names
    • v-myc myelocytomatosis viral oncogene homolog (avian)
    • Transcription factor p64
    • avian myelocytomatosis viral oncogene homolog
    • myc proto-oncogene protein
    • v-myc avian myelocytomatosis viral oncogene homolog
    背景信息 Myc-Tag is a short synthetic peptide sequence derived from C-terminal region of human c-myc that is used as an epitope tag. The Myc tag can be cloned either upstream or downstream of the target gene, resulting in a fusion protein that allows efficient purification, detection, and localization of target proteins. The relatively small size of this tag vs. other commonly used tags such as GST (30 kDa) makes it less likely to interfere with target protein structure or function. The myc epitope tag is widely used to detect expression of recombinant proteins in bacterial, yeast, insect, and mammalian systems.
    产品信息
    格式 HRP
    控制
    • Positive control for human Myc: Widespread expression including A431 cell lysate, ovarian cancer cell lysate, or breast carcinoma tissue. Myc fusion protein expressed in cells.
    演示 Purified mouse monoclonal IgG1 conjugated to horseradish peroxidase (HRP) in buffer containing: 50% storage buffer (PBS, 0.05% Kathon®, 0.2 mg/mL BSA) and 50% glycerol.
    应用
    应用 Anti-Myc Tag Antibody, clone 4A6, HRP conjugate detects level of Myc Tag & has been published & validated for use in WB.
    主要应用
    • Western Blotting
    生物信息
    免疫原品种 KLH-conjugated, synthetic peptide corresponding to amino acids 410-420 (MEQKLISEEDL) of human Myc. Clone 4A6.
    表位 C-terminal
    克隆 4A6
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Mouse
    特异性 Recognizes and is specific for recombinant proteins containing the Myc epitope tag (EQKLISEEDL) in a variety of sequence contexts. Also recognizes human Myc.
    品种反应性 Human
    Species Reactivity Note Human. Other species cross-reactivity not tested.
    抗体类型 Monoclonal Antibody
    Entrez基因编号
    • NP_002458
    Entrez基因汇总 The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene.
    基因符号
    • c-Myc
    • MYC
    • C-MYC
    纯化方法 Protein G purfied
    UniProt编号
    • P01106
    UniProt汇总 FUNCTION: SwissProt: P01106 # Participates in the regulation of gene transcription. Binds DNA both in a non-specific manner and also specifically to recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.
    SIZE: 439 amino acids; 48804 Da
    SUBUNIT: Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with TAF1C and SPAG9. Interacts with PARP10. Interacts with JARID1A and JARID1B.
    SUBCELLULAR LOCATION: Nucleus.
    PTM: Phosphorylated by PRKDC.
    DISEASE: SwissProt: P01106 # Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors. & A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
    SIMILARITY: SwissProt: P01106 ## Contains 1 basic helix-loop-helix (bHLH) domain.
    产品使用声明
    质量保证 Evaluated by Western Blot on Myc-PP2A A subunit transfected NIH/3T3 lysates.

    Western Blot Analysis:
    1:500 dilution of this antibody detected myc-PP2A on 10 µg of Myc-PP2A A subunit transfected NIH/3T3 lysates.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 1 year at -20ºC from date of receipt.
    包装信息
    数量 100 µL

    merck millipore,默克密理博,16-213,Anti-Myc Tag Antibody, clone 4A6, HRP conjugate

上一件merck millipore产品:merck millipore,默克密理博,09-031,Anti-CBX8 Antibody
下一件merck millipore产品:merck millipore,默克密理博,MABN706,Anti-ZNF804A Antibody, clone 10F8.2



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