merck millipore,默克密理博,05-485,Anti-phospho-STAT3 (Tyr705) Antibody, clone 9E12

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merck millipore,默克密理博,05-485,Anti-phospho-STAT3 (Tyr705) Antibody, clone 9E12
产品名称：Anti-phospho-STAT3 (Tyr705) Antibody, clone 9E12
产品型号：05-485
Use Anti-phospho-STAT3 (Tyr705) Antibody, clone 9E12 (Mouse Monoclonal Antibody) validated in ELISA, IP, WB to detect phospho-STAT3 (Tyr705) also known as Acute-phase response factor or DNA-binding pr More>>

merck millipore,默克密理博,05-485,Anti-phospho-STAT3 (Tyr705) Antibody, clone 9E12

产品介绍

merck millipore,默克密理博,05-485,Anti-phospho-STAT3 (Tyr705) Antibody, clone 9E12

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
Ca, H, M	WB, ELISA	M	Purified	Monoclonal Antibody

描述
产品目录编号	05-485
Replaces	04-1059
品牌系列	Upstate
商名	Upstate
描述	Anti-phospho-STAT3 (Tyr705) Antibody, clone 9E12
Alternate Names	signal transducer and activator of transcription 3 (acute-phase response factor) Acute-phase response factor DNA-binding protein APRF signal transducer and activator of transcription 3
背景信息	STATs (signal transducers and activators of transcription) were originally discovered as two proteins (STAT1 and STAT2) which were involved in interferon-alpha (IFN-alpha) and IFN-gamma signal transduction. Since then, several additional STAT proteins have been identified (STAT3, 4, 5a, 5b, and 6). STATs undergo tyrosine phosphorylation in response to growth factor or cytokine signaling. This phosphorylation results in dimerization and translocation of STAT proteins to the nucleus. In some cases this process is mediated by JAK Kinases (Janus Kinases 1, 2, and 3). For maximum activation of these proteins, phosphorylation at specific tyrosine and serine residues may be required in STAT1 alpha, 3, 4, and 5. Specific functions of the various members of the STAT family are poorly understood. STAT3 has been shown to be activated by IFN-alpha but not IFN-beta. The transcription factors associated with STAT3 are c-Jun and cyclic AMP-responsive enhancer binding protein (CREB). Deletion of the STAT3 gene in knock-out mice was lethal at the early embryonic stage.

产品信息
格式	Purified
控制	murine macrophage 4/4 cells, serum-starved for 16 hours then stimulated with Phorbol 12-myristate 13-acetate (PMA) for 10 minutes SK-N-MC cells
演示	Lyophilized purified mouse IgG1ќ from 1 mL 2x PBS / 0.09 % Na-azide / PEG and Sucrose. Reconstitute with 100 µL H2O (15 min, RT) for a final concentration of 1 mg/mL.

应用
应用	Use Anti-phospho-STAT3 (Tyr705) Antibody, clone 9E12 (Mouse Monoclonal Antibody) validated in ELISA, IP, WB to detect phospho-STAT3 (Tyr705) also known as Acute-phase response factor or DNA-binding protein APRF.
主要应用	Western Blotting ELISA
应用说明	Immunoprecipitation: An independent laboratory has reported 1-10 µg/mL of a previous lot of this antibody immunoprecipitated phospho-STAT3 (Tyr705) from a lysate of 106 vanadate-treated human A431 cells. ELISA: 0.05 µg/mL of this antibody is recommended by an independent lab for ELISA testing. Test was conducted on a previous lot.

生物信息
免疫原品种	KLH-conjugated phosphopeptide containing the AP[pY]LK motif of STAT3
克隆	9E12
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Mouse
特异性	This antibody recognizes STAT3, Mr 92 kDa, phos-phorylated on tyrosine-705. Clone 9E12.
同种型	IgG1κ
品种反应性	CanineHumanMouse
Species Reactivity Note	Human, mouse and dog
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_139276
Entrez基因汇总	The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq]. Transcript Variant: This variant (2) lacks a segment in the 5' UTR and 3 nt within the CDS, as compared to variant 1. The resulting isoform (2) lacks an amino acid compared to isoform 1.
基因符号	MGC16063 STAT3 APRF FLJ20882
修改	Phosphorylation
纯化方法	Thiophilic adsoption and size exclusion chromatography
UniProt编号	P40763
UniProt汇总	FUNCTION: SwissProt: P40763 # Transcription factor that binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA. SIZE: 770 amino acids; 88068 Da SUBUNIT: Forms a homodimer or a heterodimer with a related family member (at least STAT1). Interacts with NCOA1, PELP1, SOCS7 and STATIP1. Interacts with HCV core protein. Interacts with IL23R in presence of IL23. Interacts with IL31RA. SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Translocated into the nucleus in response to phosphorylation. TISSUE SPECIFICITY: Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. PTM: Tyrosine phosphorylated in response to IL-6, IL-11, CNTF, LIF, CSF-1, EGF, PDGF, IFN-alpha and OSM. Phosphorylated on serine upon DNA damage, probably by ATM or ATR. Serine phosphorylation is important for the formation of stable DNA-binding STAT3 homodimers and maximal transcriptional activity. DISEASE: "SwissProt: P40763 # Defects in STAT3 are the cause of hyperimmunoglobulin E recurrent infection syndrome autosomal dominant (AD-HIES) [MIM:147060]; also known as hyper-IgE syndrome or Job syndrome. AD-HIES is a rare disorder of immunity and connective tissue characterized by immunodeficiency, chronic eczema, recurrent Staphylococcal infections, increased serum IgE, eosinophilia, distinctive coarse facial appearance, abnormal dentition, hyperextensibility of the joints, and bone fractures." SIMILARITY: SwissProt: P40763 ## Belongs to the transcription factor STAT family. & Contains 1 SH2 domain. MISCELLANEOUS: Involved in the gp130-mediated signaling pathway." ...

产品使用声明
质量保证	Routinely evaluated by immunoblot on lysates from human SK-N-MC cells stimulated with CNTF. Western Blot Analysis: 0.1-1 µg/mL of this lot detected phospho-STAT3 (Tyr705) in lysates from human SK-NMC cells stimulated with CNTF.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.