merck millipore,默克密理博,03-100,RIPAb+™ hnRNP M1-M4

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merck millipore,默克密理博,03-100,RIPAb+™ hnRNP M1-M4
产品名称：RIPAb+™ hnRNP M1-M4
产品型号：03-100

merck millipore,默克密理博,03-100,RIPAb+™ hnRNP M1-M4

产品介绍

merck millipore,默克密理博,03-100,RIPAb+™ hnRNP M1-M4

重要规格表

品种反应性	主要应用
Rb, B, H, M, Po, R	WB

描述
产品目录编号	03-100
商名	Upstate RIPAb+
描述	RIPAb+™ hnRNP M1-M4
概述	RIPAb+ antibodies are evaluated using the RNA Binding Protein Immunoprecipitation (RIP) assay. Each RIPAb+ antibody set includes a negative control antibody to ensure specificity of the RIP reaction and is verified for the co-immunoprecipitation of RNA associated specifically with the immunoprecipitated RNA binding protein of interest. Where appropriate, the RIPAb+ set also includes quantitative RT-PCR control primers (RIP Primers) to biologically validate your IP results by successfully co-precipitating the specific RNA targets, such as messenger RNAs. The qPCR protocol and primer sequences are provided, allowing researchers to validate RIP protocols when using the antibody in their experimental context. If a target specific assay is not provided, the RIPAb+ kit is validated using an automated microfluidics-based assay by enrichment of detectable RNA over control immunoprecipitation. The hnRNP M1-M4 proteins are pre-mRNA-binding proteins that complex with heterogeneous nuclear RNA (hnRNA). These proteins associate with pre-mRNAs in the nucleus and influence pre-mRNA processing and other aspects of mRNA metabolism and transport.
背景信息	Polycomb group proteins are important for maintaining transcriptional silencing. One conserved PcG complex, PRC2, is composed of several proteins including the histone methyltransferase EZH2, the WD-repeat protein EED (Embryonic ectoderm development), and the Zn-finger protein Suz12. Transcriptional repression mediated by EED involves histone deacetylation, while the EZH2 methylates histone H3 on lysine 27. EED protein is present in four isoforms. These EED isoforms selectively associate with distinct EZH2-containing complexes, resulting in differential targeting of their associated methyltransferase activity. These complexes play a role in Hox gene silencing, X-inactivation, germline development, stem cell pluripotency and cancer metastasis.

产品信息
格式	Purified
控制	Includes negative control normal mouse IgG antibody and control primers specific for the cDNA of human hnRNP M.
演示	Anti-hnRNP M1-M4 (Mouse Monoclonal), Part # CS207316. One vial containing 50 μg of protein G purified mouse IgG1 in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide and 30% glycerol. Store at -20°C. Normal Mouse IgG, Part # CS200621. One vial containing 125 µg of purified mouse IgG in 125 µL of storage buffer containing 0.1% sodium azide. Store at -20°C. RIP Primers, hnRNP M, Part # CS207306. One vial containing 75 μL of 5 μM of each primer specific for human hnRNP M mRNA. Store at -20°C. FOR: GAG GCC ATG CTC CTG GG REV: TTT AGC ATC TTC CAT GTG AAA TCG

应用
主要应用	Western Blotting
应用说明	Immunoprecipitation from RIP lysate: Representative lot data. RIP lysate from HeLa cells (~2 X 10E7 cell equivalents per IP) was subjected to immunoprecipitation using 5 µg of either a normal mouse IgG, (Cat. # CS200621), or 5 µg of Anti-hnRNP M1-M4 antibody (Cat. # CS207316). Ten percent of the precipitated proteins (lane 1: normal mouse IgG, lane 2: hnRNP M1-M4) were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-hnRNP M1-M4 (Cat. # CS207316, 1:1000). Proteins were visualized using One-Step™ IP-Western kit (GenScript Cat. # L00231). Arrow indicates hnRNP M1-M4. (Figure 2). Automated Microfluidics-based Electrophoretic RNA Separation and Analysis (MFE): Representative lot data. RIP Lysate prepared from HeLa cells (2 X 10E7 cell equivalents per IP) were subjected to immunoprecipitation using 5 µg of either 1. normal mouse IgG (Cat. # CS200621), or 2. Anti-hnRNP M1-M4 antibody (Cat. # CS207316) and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Cat. # 17-700). Successful immunoprecipitation of hnRNP M1-M4-associated RNA was verified by automated microfluidics-based electrophoretic RNA separation and analysis. Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details. Electropherograms were generated by plotting fluorescence intensities versus migration times (Figure 3A). The virtual gel view was created from this data (Figure 3B). Western Blot Analysis: Representative lot data. HeLa nuclear extract was resolved by electrophoresis, transferred to PVDF and probed with anti-hnRNP M1-M4 (2 µg/mL). Proteins were visualized using a goat anti-mouse IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates hnRNP M1-M4. (Figure 4).

应用

主要应用

Western Blotting

应用说明

Immunoprecipitation from RIP lysate:
Representative lot data.
RIP lysate from HeLa cells (~2 X 10E7 cell equivalents per IP) was subjected to immunoprecipitation using 5 µg of either a normal mouse IgG, (Cat. # CS200621), or 5 µg of Anti-hnRNP M1-M4 antibody (Cat. # CS207316). Ten percent of the precipitated proteins (lane 1: normal mouse IgG, lane 2: hnRNP M1-M4) were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-hnRNP M1-M4 (Cat. # CS207316, 1:1000). Proteins were visualized using One-Step™ IP-Western kit (GenScript Cat. # L00231).
Arrow indicates hnRNP M1-M4. (Figure 2).
Automated Microfluidics-based Electrophoretic RNA Separation and Analysis (MFE):
Representative lot data.
RIP Lysate prepared from HeLa cells (2 X 10E7 cell equivalents per IP) were subjected to immunoprecipitation using 5 µg of either 1. normal mouse IgG (Cat. # CS200621), or 2. Anti-hnRNP M1-M4 antibody (Cat. # CS207316) and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Cat. # 17-700).
Successful immunoprecipitation of hnRNP M1-M4-associated RNA was verified by automated microfluidics-based electrophoretic RNA separation and analysis. Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details. Electropherograms were generated by plotting fluorescence intensities versus migration times (Figure 3A). The virtual gel view was created from this data (Figure 3B).
Western Blot Analysis:
Representative lot data.
HeLa nuclear extract was resolved by electrophoresis, transferred to PVDF and probed with anti-hnRNP M1-M4 (2 µg/mL). Proteins were visualized using a goat anti-mouse IgG secondary antibody conjugated to HRP and a chemiluminescence detection system.
Arrow indicates hnRNP M1-M4. (Figure 4).

生物信息
免疫原品种	T7 gene 10 fusion protein containing full length human RNP M4
浓缩	0.7 mg/mL
宿主	Mouse
特异性	This antibody recognizes hnRNP M1-M4.
同种型	IgG1
品种反应性	RabbitBovineHumanMousePigRat
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_005968
Entrez基因汇总	This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs and also contains a nuclear localization motif. Multiple alternatively spliced transcript variants have been found for this gene.
基因符号	HNRNPR HNRPR hnRNP-R FLJ25714 HNRNP-R
UniProt编号	O43390
UniProt汇总	FUNCTION: SwissProt: O43390 # Component of ribonucleosomes, which are complexes of at least 20 other different heterogenious nuclear ribonucleoproteins (hnRNP). hnRNP play an important role in processing of precursor mRNA in the nucleus. SIZE: 633 amino acids; 70943 Da SUBUNIT: Identified in the spliceosome C complex, at least composed of AQR, ASCC3L1, C19orf29, CDC40, CDC5L, CRNKL1, DDX23, DDX41, DDX48, DDX5, DGCR14, DHX35, DHX38, DHX8, EFTUD2, FRG1, GPATC1, HNRNPA1, HNRNPA2B1, HNRPA3, HNRNPC, HNRPF, HNRPH1, HNRPK, HNRPM, HNRNPR, HNRNPU, KIAA1160, KIAA1604, LSM2, LSM3, MAGOH, MORG1, PABPC1, PLRG1, PNN, PPIE, PPIL1, PPIL3, PPWD1, PRPF19, PRPF4B, PRPF6, PRPF8, RALY, RBM22, RBM8A, RBMX, SART1, SF3A1, SF3A2, SF3A3, SF3B1, SF3B2, SF3B3, SFRS1, SKIV2L2, SNRPA1, SNRPB, SNRPB2, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, SNW1, SRRM1, SRRM2, SYF2, SYNCRIP, TFIP11, THOC4, U2AF1, WDR57, XAB2 and ZCCHC8. SUBCELLULAR LOCATION: Nucleus, nucleoplasm. SIMILARITY: SwissProt: O43390 ## Contains 3 RRM (RNA recognition motif) domains.

产品使用声明
质量保证	RNA Binding Protein Immunoprecipitation: Representative lot data. RIP Lysate prepared from HeLa cells (2 X 10E7 cell equivalents per IP) were subjected to immunoprecipitation using 5 µg of either a normal mouse IgG or 5 µg of Anti-hnRNP M1-M4 antibody and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Cat. # 17-700). Successful immunoprecipitation of hnRNP M1-M4-associated RNA was verified by qPCR using RIP Primers hnRNP M, (Figure 1). Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品使用声明

质量保证

RNA Binding Protein Immunoprecipitation:
Representative lot data.
RIP Lysate prepared from HeLa cells (2 X 10E7 cell equivalents per IP) were subjected to immunoprecipitation using 5 µg of either a normal mouse IgG or 5 µg of Anti-hnRNP M1-M4 antibody and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Cat. # 17-700).
Successful immunoprecipitation of hnRNP M1-M4-associated RNA was verified by qPCR using RIP Primers hnRNP M, (Figure 1).
Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details.

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.