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重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M, H, R WB, ICC M Purified Monoclonal Antibody 描述 产品目录编号 05-432 品牌系列 Upstate
商名 - Upstate
描述 Anti-NR1 Antibody, CT Alternate Names - glutamate receptor, ionotropic, N-methyl D-aspartate 1
- Grin1
- NMDA R1 receptor C1 cassette
- glutamate receptor
- ionotropic
背景信息 NR1 (NMDA1or NMDAR1, also known as GRIN1) along with the NR2 subunit forms a heterodimer that make up the NMDA receptor. The NMDA receptor (NMDAR) is characterized as a ionotropic receptor for glutamate. As the name suggests, (NMDA (N-methyl D-aspartate) is an agonist for the receptor. A distinct characteriztic of the NMDA receptor is that it is both ligand-gated and voltage-dependent based on its sensitivity to magnesium. Activated NMDA receptors result in the opening of an ion channel that is nonselective to cations. The result is the flow of Na+ and small amounts of Ca2+ ions into the cell and K+ out of the cell. Calcium flux by the NMDARs has been implicated in synaptic plasticity, a mechanism in the cell for learning and memory. 产品信息 格式 Purified 控制 - Brain tissue
演示 Purified mouse monoclonal IgG in buffer containing 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide. 应用 应用 Anti-NR1 Antibody, CT detects level of NR1 & has been published & validated for use in IC, IP & WB. 主要应用 - Western Blotting
- Immunocytochemistry
应用说明 Immunoprecipitation:
4 μg of a previous lot immunoprecipitated NR1 from 500mg of rat brain microsomal protein preparation.
Immunocytochemistry:
An independent laboratory has shown positive staining in QT-6 cells, transfected to express NR1, which were fixed with 4% paraformaldehyde/4% sucrose in PBS and in nontransfected cultured rat neurons.
Note: Do not boil the microsomal preparation. Incubate at room temperature for 30-45 minutes.生物信息 免疫原品种 His-tag fusion protein corresponding to residues 834-938 of rat NR1A. The immunizing sequence is identical in rat and human. 表位 The epitope maps to a region (a.a. 834-864) common to all NR1 isoforms 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 This antibody is specific for NR1, Mr 130 kDa. The epitope maps to a region (834-864) common to all NR1 isoforms and so should cross react with all NR1 isoforms. An additional band may be detected at higher concentrations, Mr 200kDa. 同种型 IgG 品种反应性 MouseHumanRat Species Reactivity Note Rat. Immunoreactivity is predicted for human and mouse. 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_017010
基因符号 - Nmdar1
- NR1
- Grin1
纯化方法 Protein G purfied UniProt编号 - P35439
UniProt汇总 FUNCTION: NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. Plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors.
SUBUNIT: Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B); disulfide-linked. Found in a complex with GRIN2A or GRIN2B, GRIN3A or GRIN3B and PPP2CB. Interacts with DLG4 and MPDZ
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein, Cell junction › synapse › postsynaptic cell membrane. Cell junction › synapse › postsynaptic cell membrane › postsynaptic density. Note: Enriched in post-synaptic plasma membrane and post-synaptic densities.
POST-TRANSLATIONAL MODIFICATION: NMDA is probably regulated by C-terminal phosphorylation of an isoform of NR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity.
SIZE: 938 amino acids; 105,509 Da产品使用声明 质量保证 Routinely evaluated by Western Blot on rat brain microsomal protein preparation.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected NR1 from 20 μg of rat brain microsomal protein preparation (Catalog # 12-144).使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2° to 8ºC from date of receipt. 包装信息 数量 200 µg
上一件merck millipore产品：merck millipore,默克密理博,OP119,Anti-c-ErbB3 (Ab-4) Mouse mAb (H3.90.6 or Ab90)
下一件merck millipore产品：merck millipore,默克密理博,616459,TGF-β RI Kinase Inhibitor VIII - CAS 356559-20-1 - Calbiochem
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