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品种反应性 主要应用 宿主 格式 抗体类型 B, H, R, M WB, ICC, IHC M Purified Monoclonal Antibody 描述 产品目录编号 05-390 品牌系列 Upstate
商名 - Upstate
描述 Anti-erbB-3/HER-3 Antibody, clone 2F12 Alternate Names - v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian)
- Tyrosine kinase-type cell surface receptor HER3
- lethal congenital contracture syndrome 2
- v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 3
背景信息 The ErbB3 gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. ErbB3 is a membrane-bound protein which has a neuregulin binding domain but not an active kinase domain. It can therefore bind this ligand but cannot convey a signal into the cell via protein phosphorylation. However it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers including prostate, bladder and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported but they have not been thoroughly characterized. 产品信息 格式 Purified 控制 - Human breast adenocarcinoma (well-differentiated), mouse brain lysate, MCF-7 cell lysate.
演示 Purified mouse monoclonal IgG2aκ in buffer containing 10 mM PBS, pH 7.4, 0.2% BSA, with 0.09% sodium azide. Frozen solution. 应用 应用 Detect erbB-3/HER-3 using this Anti-erbB-3/HER-3 Antibody, clone 2F12 validated for use in IC, IH, IP & WB. 主要应用 - Western Blotting
- Immunocytochemistry
- Immunohistochemistry
应用说明 Immunoprecipitation:
4 µg of a previous lot of this antibody immunoprecipitated erbB-3/HER-3 from human MDA and MCF-7 RIPA lysates.
Immunocytochemistry:
10 µg/mL of a previous lot of this antibody gave positive immunostaining for erbB-3/HER-3 in MCF-7cells fixed with 95% ethanol/5% acetic acid.
Immunohistochemistry:
1:50 and 1:100 dilution of a previous lot detected erbB-3/HER-3 membrane staining in human normal and carcinoma breast tissue.生物信息 免疫原品种 Recombinant rat erbB-3/HER-3 oncoprotein expressed in insect cells and purified by metal chelating chromatography. The epitope maps between amino acid residues 1295-1323 of human erbB-3. Clone 2F12. 表位 a.a. 1295-1323 of human erbB-3 克隆 2F12 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 This antibody recognizes erbB-3/HER-3, Mr 185 kDa. Does not cross-react with erbB-1 (EGFR) and erbB-2/HER-2. 同种型 IgG2aκ 品种反应性 BovineHumanRatMouse Species Reactivity Note Human. Predicted to cross-react with mouse, rat, and cow. 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_001982
Entrez基因汇总 This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized. 基因符号 - MDA-BF-1
- ERBB3
- c-erbB-3
- c-erbB3
- erbB-3
- p180-ErbB3
- p85-sErbB3
- p45-sErbB3
- ErbB-3
- erbB3-S
- MGC88033
- HER3
纯化方法 Protein A chromatography UniProt编号 - P21860
UniProt汇总 FUNCTION: SwissProt: P21860 # Binds and is activated by neuregulins and NTAK.
SIZE: 1342 amino acids; 148098 Da
SUBUNIT: Heterodimer with each of the other ERBB receptors (Potential). Interacts with CSPG5 and PA2G4.
SUBCELLULAR LOCATION: Isoform 1: Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted.
TISSUE SPECIFICITY: Epithelial tissues and brain.
DOMAIN: SwissProt: P21860 The cytoplasmic part of the receptor may interact with the SH2 or SH3 domains of many signal-transducing proteins.
PTM: Ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase (By similarity).
DISEASE: SwissProt: P21860 # Overexpressed in a subset of human mammary tumors. & Defects in ERBB3 are the cause of lethal congenital contracture syndrome type 2 (LCCS2) [MIM:607598]; also called Israeli Bedouin multiple contracture syndrome type A. LCCS2 is an autosomal recessive neurogenic form of a neonatally lethal arthrogryposis that is associated with atrophy of the anterior horn of the spinal cord. The LCCS2 syndrome is characterized by multiple joint contractures, anterior horn atrophy in the spinal cord, and a unique feature of a markedly distended urinary bladder. The phenotype suggests a spinal cord neuropathic etiology.
SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. & Contains 1 protein kinase domain.产品使用声明 质量保证 Routinely evaluated by Western Blot on RIPA lysates of human MCF-7 cells.
Western Blot Analysis:
0.1-2 µg/mL of this lot detected erbB-3/HER-3 in RIPA lysates of human MCF-7 cells. Previous lots have detected erbB-3/HER-3 in MDA RIPA lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 200 µg
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